Applied Entomology and Zoology

, Volume 49, Issue 2, pp 297–304 | Cite as

Effects of temperature on the reproduction and development of Drosophila suzukii (Diptera: Drosophilidae)

  • Hirotoshi Kinjo
  • Yasuhisa Kunimi
  • Madoka Nakai
Original Research Paper


The objective of this study was to elucidate how temperature affects the reproduction and development of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), an emerging major pest of blueberry in Japan. Although extensive studies of the biology of this pest have been carried out, the effects of temperature on its reproduction and development remain unknown. We found that when adults mated at 31 °C for 4 days, none of the eggs hatched. Female oviposition and egg hatching rate were also reduced as temperature increased during the oviposition period. When D. suzukii larvae developed above 31 °C, pupation and adult eclosion were abolished. According to field observations, adult D. suzukii ceased to appear from the end of July 2010, when the average temperature exceeded 28 °C or when the temperature within a day exceeded 33 °C for 8 h or more. Experiments in which the mating temperature fluctuated within a day revealed that both the number of eggs oviposited and their hatch rate were significantly suppressed when the daily temperature regime during mating was either 31 °C for 12 h/25 °C for 12 h or 33 °C for 8 h/25 °C for 16 h, relative to the values at 25 °C for 24 h.


Oviposition Larval development Mating 



Authors sincerely thank Drs. Isao Ogiwara, Takashi Motobayashi, Hidenobu Tsujimura, Jun Shimada, Tomoe Shimizu and Takuya Ban, Mr. Satoshi Noma and Ms. Mika Mashimo of Tokyo University of Agriculture and Technology, Dr. Koichiro Tamura of Tokyo Metropolitan University and Dr. Ian Smith. This project was partially supported by a special research fund of the Ministry of Education, Culture, Sports, Science and Technology in Japan: “Research and development of security and safe crop production to reconstruct agricultural lands in Fukushima Prefecture based on novel techniques to remove radioactive compounds using advanced bio-fertilizer and plant protection strategies” and the Ministry of Agriculture, Forestry and Fisheries in Japan: “Demonstration of off-season blueberry harvesting technique in cooperation with nursery in advanced plant factory” in A Scheme to Revitalize Agriculture and Fisheries in Disaster Area through Deploying Highly Advanced Technology.

Supplementary material

13355_2014_249_MOESM1_ESM.pdf (45 kb)
Fig. 1S Number of eggs oviposited during a day by female adult Drosophila suzukii of different ages at 25 °C. Female flies were allowed to mate on general medium just after emergence and until days 2–7 after emergence (2Days to 7Days), and were then placed individually on grape juice agar plates for 24 h for oviposition. Numbers of eggs laid per female during 24 h were counted (a), and the percentage of eggs that hatched within 48 h (b) was examined. Bars indicate standard errors. Different letters denote a significant difference (PDF 45 kb)


  1. Kanzawa T (1934) Studies on Drosophila suzukii (preliminary report). Yamanashi Prefecture Agricultural Research Station Report (preliminary report). Yamanashi Prefecture Agricultural Institute, Yamanashi, Japan, p 40 (in Japanese)Google Scholar
  2. Kanzawa T (1939) Studies on Drosophila suzukii. Yamanashi Prefecture Agricultural Research Station Report. Yamanashi Prefecture Agricultural Institute, Yamanashi, Japan, p 49 (in Japanese)Google Scholar
  3. Kawase S, Uchino K, Takahashi K (2007) Control of cherry Drosophila Drosophila suzukii injurious to blueberry. Plant Protect 61:205–209 (in Japanese)Google Scholar
  4. Kimura MT (2004) Cold and heat tolerance of drosophilid flies with reference to their latitudinal distributions. Oecologia 140:442–449. doi: 10.1007/s00442-004-1605-4 PubMedCrossRefGoogle Scholar
  5. Kinjo H, Kunimi Y, Ban T, Nakai M (2013) Oviposition efficacy of Drosophila suzukii (Diptera: Drosophilidae) on different cultivars of blueberry. J Econ Ent 106:1767–1771. doi: 10.1603/EC12505 CrossRefGoogle Scholar
  6. Lee JC, Bruck DJ, Curry H, Edwards D, Haviland DR, Van Steenwyk RA, Yorgey BM (2011a) The susceptibility of small fruits and cherries to the spotted-wing drosophila, Drosophila suzukii. Pest Manag Sci 67:1358–1367. doi: 10.1002/ps.2225
  7. Lee JC, Bruck DJ, Dreves AJ, Ioriatti C, Vogt H, Baufeld P (2011b) In Focus: spotted wing drosophila, Drosophila suzukii, across perspectives. Pest Manag Sci 67:1349–1351. doi: 10.1002/ps.2271 PubMedCrossRefGoogle Scholar
  8. Mitsui H, Beppu K, Kimura M (2010) Seasonal life cycles and resource uses of flower- and fruit-feeding drosophilid flies (Diptera: Drosophilidae) in central Japan. Entomol Sci 13:60–67. doi: 10.1111/j.1479-8298.2010.00372.x CrossRefGoogle Scholar
  9. Salvaterra PM, Hayashi I, Perez-Magallanes M, Ikeda K (2006) Primary culture of Drosophila embryo cells. In: Celis JE (ed) Cell biology, a laboratory handbook, 3rd edn, vol 1. Elsevier Science, Burlington, pp 151–155Google Scholar
  10. Sasaki M, Abe N (1993) Occurrence of Drosophila in the cherry orchards (1) Species and the seasonal prevalence obtained by the bait trap. Ann Rept Plant Prot North Japan 44:169–171 (in Japanese)Google Scholar
  11. Sasaki M, Sato R (1995) Bionomics of the cherry Drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae) in Fukushima Prefecture. 1. Drosophila injured on cherry fruit. Ann Rept Plant Prot North Japan 46:164–166 (in Japanese)Google Scholar
  12. Shimizu K (2006) Occurrence and control of the cherry Drosophila, Drosophila suzukii (Matsumura) in blue berry fruit. Plant Protect 60:103–106 (in Japanese)Google Scholar
  13. Takamori H, Watabe H, Fuyama Y, Zhang Y, Aotsuka T (2006) Drosophila subpulchrella, a new species of the Drosophila suzukii species subgroup from Japan and China (Diptera: Drosophilidae). Entomol Sci 9:121–128. doi: 10.1111/j.1479-8298.2006.00159.x CrossRefGoogle Scholar
  14. Walsh DB, Bolda MP, Goodhue RE, Dreves AJ, Lee J, Bruck DJ, Walton VM, O’Neal SD, Zalom FG (2011) Drosophila suzukii (Diptera: Drosophilidae): invasive pest of ripening soft fruit expanding its geographic range and damage potential. J Integr Pest Manag 2:1–7. doi: 10.1603/IPM10010 CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Applied Entomology and Zoology 2014

Authors and Affiliations

  • Hirotoshi Kinjo
    • 1
    • 2
  • Yasuhisa Kunimi
    • 1
  • Madoka Nakai
    • 1
  1. 1.Graduate School of AgricultureTokyo University of Agriculture and TechnologyTokyoJapan
  2. 2.Mitsui Chemicals, Agro. Ltd., Institute of Agricultural ChemistryMobaraJapan

Personalised recommendations