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Isolation and characterization of 14 microsatellite markers in Macrodasyceras hirsutum (Hymenoptera: Torymidae)

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Abstract

Macrodasyceras hirsutum Kamijo is the seed parasitoid wasp of the bird-dispersed, dioecious tree, Ilex integra Thunb. The wasp reduces the level of dispersal mutualism between the Ilex tree and its frugivorous birds by manipulating the color of mature berries. The female trees do not blossom every year and sometimes change sex. Thus, the reproduction biology of I. integra affects the population size and structure of M. hirsutum in a forest and consequently influences the seed dispersal mutualism between the tree and birds, because of limited ability of adult locomotion. To investigate the wasp population structure with reference to the dispersal mutualism between trees and birds, we isolated 14 microsatellite loci of M. hirsutum wasps. Every locus was polymorphic among 20 females, with 3–13 alleles per locus, without linkage disequilibrium. The observed and expected heterozygosities ranged from 0.100 to 0.900 and 0.099 to 0.818, respectively, indicating their utility in molecular analyses of the wasp population.

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Acknowledgments

E. Takagi was supported by a fellowship and research funding from the Japan Society for the Promotion of Science.

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Author notes

  1. Etsuro Takagi

    Present address: Sugadaira Montane Research Center, University of Tsukuba, Sugadaira Kogen, Ueda, Nagano, 386-2204, Japan

Authors and Affiliations

  1. Laboratory of Forest Zoology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Etsuro Takagi & Katsumi Togashi

  2. Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Norihisa Matsushita & Taizo Hogetsu

Authors
  1. Etsuro Takagi
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  2. Norihisa Matsushita
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  3. Katsumi Togashi
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  4. Taizo Hogetsu
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Correspondence to Etsuro Takagi.

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Takagi, E., Matsushita, N., Togashi, K. et al. Isolation and characterization of 14 microsatellite markers in Macrodasyceras hirsutum (Hymenoptera: Torymidae). Appl Entomol Zool 49, 197–200 (2014). https://doi.org/10.1007/s13355-013-0237-8

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  • DOI: https://doi.org/10.1007/s13355-013-0237-8

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