Abstract
Anamorphic Beauveria are cosmopolitan entomopathogenic fungi that parasitize a broad range of insect species in virtually all terrestrial habitats. A diversity survey of 189 exemplar strains of Beauveria from the RCEF culture collection, representative of its taxonomic diversity, geographic distribution and insect host range in China, was conducted based on a combination of DGGE genotyping and nucleotide sequence analysis of the Bloc nuclear intergenic region. The DGGE assays detected 42 electrophoretically distinct haplotypes, with each haplogroup including 1–13 individuals. Nucleotide sequence analysis established that all haplogroups were uniquely distinguished by one or more nucleotide differences and that isolates from the same DGGE haplogroup share sequence identity. A phylogenetic analysis inclusive of this Bloc haplotype diversity assigned the Chinese Beauveria strains to six species lineages corresponding to B. bassiana sensu lato. (Bals.) Vuill, B. brongniartii (Sacc.) Petch, B. australis S.A. Rehner & Humber, B. asiatica S.A. Rehner & Humber, B. pseudobassiana S.A. Rehner & Humber and B. caledonica Bissett & Widden. B. australis is reported for the first time in China. This study represents the first phylogenetic survey of Beauveria species diversity in China, and demonstrates a simple and effective screening strategy to facilitate the identification of Beauveria genotypes.
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Acknowledgments
We are grateful to Drs. Mingjun Chen and Shengli Zhang for their kind help with the collection and isolation of Beauveria samples. This work was supported by a grant from the Natural Science Foundation of China (nos. 31070009 and 30300004).
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Cai, Y., Pu, S., Nie, Y. et al. Discrimination of Chinese Beauveria strains by DGGE genotyping and taxonomic identification by sequence analysis of the Bloc nuclear intergenic region. Appl Entomol Zool 48, 255–263 (2013). https://doi.org/10.1007/s13355-013-0179-1
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DOI: https://doi.org/10.1007/s13355-013-0179-1