Abstract
We have developed and characterized 21 microsatellite markers in the aphidophagous gall midge Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae). All 21 loci tested were polymorphic: the number of alleles ranged from 2 to 17. Allelic richness and observed heterozygosities were higher in females than in males. Several loci had no heterozygosity in males, suggesting that the loci were located on sex chromosomes or E-chromosomes, common to cecidomyiids. The high polymorphism detected in this study suggests the markers will be of value in analyzing genetic structure of field populations.
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References
Eilenberg J, Hajek A, Lomer C (2001) Suggestions for unifying the terminology in biological control. BioControl 46:387–400
Gilkeson LA, Hill SB (1987) Release rates for control of green peach aphid (Homoptera: Aphididae) by the predatory midge Aphidoletes aphidimyza (Diptera: Cecidomyiidae) under winter greenhouse conditions. J Econ Entomol 80:147–150
Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486
Goudet J (2001) Fstat, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available at: http://www2.unil.ch/popgen/softwares/fstat.htm. Accessed 3 Apr 2012
Gruzova M, Batalova F (1993) Oogenesis and meiotic divisions of predatory gall midge, Aphidoletes aphidimyza Rond. (Diptera : Cecidomyiidae). Int J Insect Morphol Embryol 22:315–334
Hamilton MB, Pincus EL, Fiore AD, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. BioTechniques 27:500–507
Hancock J (1999) Microsatellites and other simple sequences: genomic context and mutational mechanisms. In: Goldstein DB, Schlötterer C (eds) Microsatellites: evolution and applications. Oxford University Press, Oxford, pp 1–9
Harris KM (1973) Aphidophagous Cecidomyiidae (Diptera): taxonomy, biology, and assessments of field populations. Bull Ent Res 63:305–325
Hinomoto N, Higaki T, Noda T (2009) Development of microsatellite markers for the minute pirate bug Orius sauteri (Poppius), and their cross-species amplification in O. minutus (L.) and O. strigicollis (Poppius) (Heteroptera: Anthocoridae). Appl Entomol Zool 44:635–642
Hinomoto N, Todokoro Y, Higaki T (2011) Population structure of the predatory mite Neoseiulus womersleyi in a tea field based on an analysis of microsatellite DNA markers. Exp Appl Acarol 53:1–15
MacDonald C, Brookes CP, Edwards KJ, Baker DA, Lockton S, Loxdale HD (2003) Microsatellite isolation and characterization in the beneficial parasitoid wasp Diaeretiella rapae (M’Intosh) (Hymenoptera: Braconidae: Aphidiinae). Mol Ecol Notes 3:601–603
Meadow RH, Kerry WC, Shelton AM (1985) Evaluation of Aphidoletes aphidimyza (Dip.: Cecidomyiidae) for control of Myzus persicae (Hom.: Aphididae) in greenhouses and field experiments in the United States. Entomophaga 30:385–392
Mousadik AE, Petit RJ (1996) High level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L.) Skeels] endemic to Morocco. Theor Appl Genet 92:832–839
Pimentel D (2008) Conservation biological control. Biol Control 45:171
Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for windows and linux. Mol Ecol Notes 8:103–106
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schlötterer C, Pemberton J (1998) The use of microsatellites for genetic analysis of natural populations—a critical review. In: DeSalle R, Schierwater B (eds) Molecular approaches to ecology and evolution. Birkhäuser Verlag, Basel, pp 71–86
Shirota Y, Iituka K, Asano J, Abe J, Yukawa J (1999) Intraspecific variations of mitochondrial cytochrome oxidase I sequence in an aphidophagous species, Aphidoletes aphidimyza (Diptera : Cecidomyiidae). Entomol Sci 2:209–215
Solarska E (2004) The use of Aphidius colemani and Aphidoletes aphidimyza to control damson-hop aphid (Phorodon humuli Schrank) on hop. J Plant Prot Res 44:85–90
Stuart JJ, Hatchett JH (1988) Cytogenetics of the Hessian fly: I. Mitotic karyotype analysis and polytene chromosome correlations. J Hered 79:184–189
Yamane M, Yano E, Matsumoto Y, Yoshioka S, Kawai T, Toyonishi H, Nakamura T (2012) Effect of photoperiod and temperature on the induction of diapause in a Japanese strain of Aphidoletes aphidimyza (Diptera: Cecidomyiidae). Appl Entomol Zool 47:17–26
Yukawa J, Abe J, Mizota K (2008) Improvement in the practical use of an aphidophagous gall midge, Aphidoletes aphidimyza (Diptera: Cecidomyiidae), in greenhouses. In: Mason PG, Gillespie DR, Vincent C (eds) Proceedings of ISBCA 3, pp 77–78
Yukawa J, Yamaguchi D, Mizota K, Setokuchi O (1998) Distribution and host range of an aphidophagous species of Cecidomyiidae, Aphidoletes aphidimyza (Diptera), in Japan. Appl Entomol Zool 33:185–193
Zhou Y, Gu H, Dorn S (2005) Polymorphic microsatellite loci in the parasitic wasp Cotesia glomerata (Hymenoptera: Braconidae). Mol Ecol Notes 5:475–477
Acknowledgments
This research was supported by a Grant-in-Aid for Scientific Research (B) (no. 21380201) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank Drs Hideaki Inoue, Yositaka Sakamaki, and Kazuki Kakimoto, and Mr. Hiroaki Toyonishi for their help in collecting field samples.
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Hinomoto, N., Higaki, T., Abe, J. et al. Development and characterization of 21 polymorphic microsatellite loci in the aphidophagous gall midge, Aphidoletes aphidimyza (Diptera: Cecidomyiidae). Appl Entomol Zool 47, 165–171 (2012). https://doi.org/10.1007/s13355-012-0104-z
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DOI: https://doi.org/10.1007/s13355-012-0104-z