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Isolation and characterization of microsatellite loci from Spodoptera exigua (Lepidoptera: Noctuidae)

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Abstract

Ten polymorphic microsatellite loci were isolated and characterized from Spodoptera exigua (Hübner), which is a worldwide economic pest on various crops. The isolated loci were polymorphic, with 3–15 alleles in 40 individuals from four regional populations in Korea. The analyses revealed that 40 individuals had different multilocus genotypes, with the expected heterozygosity ranging from 0.133 to 0.899. Eight of the ten loci did not deviate significantly from Hardy-Weinberg equilibrium. The isolated markers will facilitate population genetic studies of S. exigua.

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Acknowledgments

This study was supported by the Cooperative Research Program for Agricultural Science and Technology Development (Project No. 200901FHT051430521), RDA, Republic of Korea. We thanks Mr. J.I. Kim, Highland Agriculture Research Center, National Institute of Crop Science, RDA, Dr. Y.M. Park, Jungbu Regional Office, Animal, Plant and Fisheries Quarantine and Inspection Agency, and Mr. S.Y. Kim, Crop Protection, Dongbu Hannong, for their help in supplying specimens. H. Kim was supported by RP-Grant 2011 of Ewha Womans University.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Science, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea

    Minyoung Kim, Deok Ho Kwon & Seunghwan Lee

  2. Research Institute of EcoScience and Division of EcoScience, Department of Life Sciences, Ewha Womans University, 11-1, Daehyeon, Seodaemun, Seoul, 120-750, Republic of Korea

    Hyojoong Kim

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  1. Minyoung Kim
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  2. Hyojoong Kim
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  3. Deok Ho Kwon
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  4. Seunghwan Lee
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Correspondence to Seunghwan Lee.

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M. Kim and H. Kim contributed equally to this research.

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Kim, M., Kim, H., Kwon, D.H. et al. Isolation and characterization of microsatellite loci from Spodoptera exigua (Lepidoptera: Noctuidae). Appl Entomol Zool 47, 149–152 (2012). https://doi.org/10.1007/s13355-012-0094-x

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