Abstract
ATP-binding cassette (ABC) transporters are widespread among organisms, and 56 genes encode ABC transporters in Drosophila melanogaster (Meigen). Their functions are thought to be divergent. In this study, we examined whether there is a clock-related ABC transporter by performing genome-wide screening using tissue-specific RNA interference. We obtained five candidates when we used tim(UAS)-gal4, which expresses in virtually all clock-related cells. Because their phenotypes were principally reproducible even when we used pdf-gal4, which expresses in a subset of pacemaker neurons only, those transporters were presumed to function in pacemaker neurons. Those five candidates can be categorized into two groups according to the phenotype of the knockdown flies. In one group, CG9281 and CG15410 (E23), the circadian period of knockdown flies was altered. In the other group, CG5944, CG6052, and CG18633, some of the knockdown flies became arrhythmic whereas for others rhythmicity remained intact. Our results suggest that some ABC transporters that have a significant function in the Drosophila circadian system.
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Acknowledgments
We thank Justin Blau for the tim(UAS)-gal4 line and Jeffrey C. Hall for the pdf-gal4 line. The UAS strains were obtained from the Genetic Strains Research Center at the National Institute of Genetics and the Vienna Drosophila RNAi Center. We thank Kenji Tomioka, Takahisa Miyatake, and Hideharu Numata for helpful discussion, and Bruce Allen for critical reading of an earlier version of this manuscript. This work is supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A.M.
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Itoh, T.Q., Matsumoto, A. Genome-wide RNA interference screening for the clock-related gene of ATP-binding cassette transporters in Drosophila melanogaster (Diptera: Drosophilidae). Appl Entomol Zool 47, 79–86 (2012). https://doi.org/10.1007/s13355-012-0091-0
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DOI: https://doi.org/10.1007/s13355-012-0091-0