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Pathogenicity of microorganisms isolated from the oak platypodid, Platypus quercivorus (Murayama) (Coleoptera: Platypodidae)

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Abstract

The oak platypodid, Platypus quercivorus (Murayama), is the most destructive pest of Quercus and related fagaceous species in Japan. To find an effective biocontrol agent, we determined the effectiveness of entomopathogenic microorganisms against P. quercivorus. P. quercivorus-infested Oak (Quercus serrata, Q. crispula and Q. acutissima) logs were obtained from various regions in Japan, and 28 isolates of microorganisms were isolated from P. quercivorus larvae from those logs. Using molecular methods, the microbes were then classified into 18 species. After a bioassay, we found that three fungi, Lecanicillium sp., Isaria tenuipes, Beauveria bassiana, and the bacterium Serratia marcescens had the greatest potential as biological control agents against P. quercivorus. Another bioassay was then performed to determine the effect of spore or cell densities on the pathogenicity of the four microorganisms, and optimum spore or cell densities for the microbes were determined to be 106 and 107/ml. Based on the results and other criteria, we tentatively selected B. bassiana as the best candidate for future use against P. quercivorus larvae.

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Acknowledgments

We thank Dr. Jack Sutherland for reviewing our manuscript and Dr. Kobayashi and the many foresters of Kyoto, Kagoshima, Shimane, Tottori, Wakayama, Gifu, Ishikawa, Toyama, Nagano, and Yamagata prefectures for collecting and sending Quercus log samples. We also thank Mr. Sanada, Mr. Shinya, and other members of our laboratory for their kind help with the studies.

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Authors and Affiliations

  1. Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Hong-ye Qi, Rikiya Endoh, Yuko Takeuchi & Kazuyoshi Futai

  2. Department of Plant Protection, College of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China

    Jian-guo Wang

  3. Department of Plant Protection, Faculty of Agriculture, University of Brawijaya, Jl. Veteran, Malang, Jawa Timur, 65145, Indonesia

    Hagus Tarno

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  1. Hong-ye Qi
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  2. Jian-guo Wang
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  3. Rikiya Endoh
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  4. Yuko Takeuchi
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  6. Kazuyoshi Futai
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Correspondence to Kazuyoshi Futai.

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Qi, Hy., Wang, Jg., Endoh, R. et al. Pathogenicity of microorganisms isolated from the oak platypodid, Platypus quercivorus (Murayama) (Coleoptera: Platypodidae). Appl Entomol Zool 46, 201–210 (2011). https://doi.org/10.1007/s13355-011-0032-3

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