Abstract
The chloroplast genomes of five Fritillaria ussuriensis materials from different production areas were comparatively analyzed, atpF and petB were screened as specific DNA barcodes, and the population identification and genetic diversity of F. ussuriensis were analyzed based on them. The F. ussuriensis chloroplast genome showed a total length of 151 515–151 548 bp with a typical tetrad structure and encoded 130 genes. atpF and petB were used to amplify 183 samples from 13 populations, and they could identify 6 and 9 haplotypes, respectively. Joint analysis of the two sequences revealed 18 haplotypes, named H1–H18, with the most widely distributed and most abundant being H4. Ten haplotypes were unique for 7 populations that they could be used to distinguish from others. Haplotype diversity and nucleotide diversity were 0.99 and 2.09 × 10−3, respectively, indicating the genetic diversity was relatively rich. The results of the intermediary adjacency network showed that H5 was the oldest haplotype, and stellate radiation was centered around it, indicating that population expansion occurred in genuine production areas. This study lays a theoretical foundation for the population identification, genetic evolution, and breed selection of F. ussuriensis.
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Data availability
cpDNA sequences determined in this study were deposited in NCBI GenBank under the accession numbers OR723982-OR723986. atpF sequences determined in this study were deposited in NCBI GenBank under the accession numbers OR767291-OR767296. petB sequences determined in this study were deposited in NCBI GenBank under the accession numbers OR767298-OR767306.
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Acknowledgements
We would like to express our special thanks to Shengli Wei and Xiaohui Wang for conceiving and designing the research. Xin Wang and Zhifei Zhang conducted experiments and wrote the manuscript. Gaojie He, Kelu An, Laha Amu, Wenqin Chen, and Ziqi Liu collected the samples. Jinhui Man, Yue Shi, Yuying Huang, Xiaoqin Zhang, and Shanhu LIU analyzed the data.
Funding
This study was supported by grants from study on breeding and cultivation technology of precision medicine Bupleurum bupleurum based on molecular anti-counterfeiting technology (20221156), research project on molecular anti-counterfeiting technology of 4 precision medicinal materials such as Platycodon grandiflorus (2020110031009385), and National Key Research and Development Program of China (2022YFC3501505).
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Wang, X., Zhang, Z., Shi, Y. et al. Population identification and genetic diversity analysis of Fritillaria ussuriensis (Fritillaria) based on chloroplast genes atpF and petB. J Appl Genetics (2024). https://doi.org/10.1007/s13353-024-00874-z
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DOI: https://doi.org/10.1007/s13353-024-00874-z