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lncRNA MIAT promotes luminal B breast cancer cell proliferation, migration, and invasion in vitro

  • Human Genetics • Original Paper
  • Published:
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Abstract

Long noncoding RNAs (lncRNAs) play a role in the emergence and progression of several human tumors, including luminal B breast cancer (BC). The biological functions and potential mechanisms of lncRNA myocardial infarction-associated transcripts (MIAT) in luminal B BC, on the contrary, are unknown. In this work, we used UALCAN database analysis to find high expression of lncRNA MIAT in luminal BC tissues and also confirmed high levels of lncRNA MIAT expression in luminal B BC tissues and cells. In vitro knockdown of MIAT inhibited the proliferation, migration, and invasion of BT474 cells. In addition, we found that miR-150-5p levels were significantly reduced in luminal B BC specimens and cells, and miR-150-5p levels were significantly increased when MIAT was knocked down. And TIMER database analysis showed that MIAT was positively associated with PDL1. Through bioinformatic tools and in vitro experiments, lncRNA MIAT could function as a competitive endogenous RNA (CeRNA) to further regulate programmed cell death ligand 1 (PDL1) expression by directly sponging miR-150-5p. In conclusion, our data suggest that MIAT, an oncogene, may sponge miR-150-5p to regulate PDL1 expression and affect proliferation, migration, and invasion in luminal B BC in vitro.

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Data are available in request from corresponding author.

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Funding

This work was supported by the “Xinglin Scholars” discipline talent research improvement program of Chengdu University of Traditional Chinese Medicine (YYZX2021018).

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Authors and Affiliations

  1. Molecular Immunology, College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan, China

    Jintao Mi & Hongsheng Zhang

  2. Department of Breast Surgery, People’s Hospital of Deyang City, Deyang, 618000, Sichuan, China

    Xuemei Jiang & Ying Yi

  3. Department of Clinical Laboratory, People’s Hospital of Deyang City, Deyang, 618000, Sichuan, China

    Weiwei Cao, Chunjiao Song & Chengliang Yuan

Authors
  1. Jintao Mi
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  2. Hongsheng Zhang
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  3. Xuemei Jiang
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  4. Ying Yi
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  5. Weiwei Cao
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  6. Chunjiao Song
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  7. Chengliang Yuan
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Contributions

CS and CY conceived and designed this study; YY and XJ provided the study materials; HZ collected and summarized the data; WC analyzed and explained the data; JM performed the experiments. All authors participated in the writing of the final manuscript.

Corresponding author

Correspondence to Chengliang Yuan.

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The research was approved by the Ethics Committee of Deyang People’s Hospital.

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The authors declare no competing interests.

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Communicated by: Ewa Ziętkiewicz

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Mi, J., Zhang, H., Jiang, X. et al. lncRNA MIAT promotes luminal B breast cancer cell proliferation, migration, and invasion in vitro. J Appl Genetics 65, 309–319 (2024). https://doi.org/10.1007/s13353-023-00807-2

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  • DOI: https://doi.org/10.1007/s13353-023-00807-2

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