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Virulence genes, phylogenetic analysis, and antimicrobial resistance of Escherichia coli isolated from urinary tract infection in hospitalized patients and outpatients

Abstract

Uropathogenic Escherichia coli (UPEC) strains are the most common cause of urinary tract infection (UTI) in hospitalized and community patients. The aim was to compare the genetic characteristics of E. coli isolated from inpatients (IPs) and outpatients (OPs) with UTI regarding their phylogenies, virulence traits, and resistance trends. In this cross-sectional study, 130 epidemiologically unrelated E. coli isolates were collected from patients with UTI. Extended-spectrum beta-lactamase (ESBL) production was detected by the combination disk method. UPEC and intestinal pathogenic E. coli (IPEC) virulence genes were detected by polymerase chain reaction. The isolates were analyzed for phylogenetic grouping. A P value of < 0.05 was considered significant. Of the 130 isolates, 62.3% were from OPs and 37.7% from IPs. About 35.8% of the OPs and 49% of the IPs were ESBL positive. Moreover, 56.8% of the OPs and 59.2% of the IPs were positive for UPEC virulence genes. Notably, 50% of the isolates from each group exhibited IPEC virulence properties. The predominant phylogroup was B2 (43.2% in the OPs and 40.8% in the IPs). No significant difference was found between the IP and OP isolates (P > 0.05). Our results may indicate that consideration should also be given to hygienic standards in the community. The marked genetic plasticity of E. coli has allowed the emergence of strains showing arrays of genes from different pathotypes. Characterization of E. coli isolates in different areas may guide the selection of effective infection control strategies.

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Funding

This study was supported by a grant [IR.MUK.REC.1398.244] from the Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.

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Correspondence to Safoura Derakhshan.

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IR.MUK.REC.1398.244, Research Ethics Committee (REC), Faculty of Medicine, Kurdistan University of Medical Sciences.

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The authors declare no competing interests.

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Communicated by Agnieszka Szalewska-Palasz.

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Derakhshan, S., Saedi, S., Ahmadi, A. et al. Virulence genes, phylogenetic analysis, and antimicrobial resistance of Escherichia coli isolated from urinary tract infection in hospitalized patients and outpatients. J Appl Genetics (2022). https://doi.org/10.1007/s13353-022-00718-8

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  • DOI: https://doi.org/10.1007/s13353-022-00718-8

Keywords

  • Urinary tract
  • Pathogenic Escherichia coli
  • Phylogenetic group
  • Community-acquired infection