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Discovery of genomic regions associated with resistance to late wilt disease caused by Harpophora maydis (Samra, Sabet and Hing) in maize (Zea mays L.)

  • Plant Genetics • Original Paper
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Abstract

Late wilt disease (LWD) caused by Harpophora maydis (Samra, Sabet and Hing) is emerging as major production constraint in maize across the world. As a prelude to develop maize hybrid resistance to LWD, genetic basis of resistance was investigated. Two F2:3 mapping populations (derived from CV156670 × 414-33 (P-1) and CV156670 × CV143587 (P-2)) were challenged with LWD at two locations (Kallinayakanahalli and Muppadighatta) during 2017 post-rainy season. A wider range of LWD scores was observed at both locations in both the populations. LWD response was influenced by significant genotype × location interaction. Six and 56 F2:3 progeny families showed resistance level better than resistant parent. A total of 150 and 199 polymorphic single nucleotide polymorphism markers were used to genotype P-1 and P-2, respectively. Inclusive composite interval mapping was performed to detect significant Quantitative Trait Loci (QTL), QTL × QTL, QTL × location interaction effects. Three major and four minor QTL controlling LWD resistance were detected on chromosome-1. The position and effect of the QTL varied with the location. Significant di-QTL interactions involving QTL (with significant and/or non-significant effects) located within and between all the ten chromosomes were detected. Five of the seven detected QTL showed significant QTL × location interaction. Though two major QTL (q-lw-1.5 and q-lw-1.6) with lower Q×L interaction effects could be considered as stable, their phenotypic variance is not large enough to deploy them in Marker Assisted Selection (MAS). However, these QTL are of paramount importance in accumulating positive alleles for LWD resistance breeding.

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Acknowledgements

Extension of field and lab facilities by Monsanto India Limited under collaborated project with University of Agricultural Sciences, Bengaluru, Karnataka, India is gratefully acknowledged.

Funding

This research was funded by Monsanto India Limited under collaborated project with University of Agricultural Sciences, Bengaluru, Karnataka, India including financial support in the form of ‘Monsanto student fellowship’.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra, Bengaluru, 560065, India

    N. C. Sunitha, E. Gangappa & S. Ramesh

  2. Bayer Crop Science, Bengaluru, India

    R. P. Veeresh Gowda, Sunil Biradar, Dharanendra Swamy & H. B. Hemareddy

Authors
  1. N. C. Sunitha
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  2. E. Gangappa
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  3. R. P. Veeresh Gowda
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  4. S. Ramesh
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  5. Sunil Biradar
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  6. Dharanendra Swamy
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  7. H. B. Hemareddy
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Contributions

EG, VRPG and SR conceptualized the work. SNC conducted experiments and analyses. DS and SNC performed inoculation and screening for late wilt disease. SB, SR and SNC interpreted results. SNC and SR revised the manuscript. EG, SR, VRPG and HBH reviewed and edited the final version.

Corresponding author

Correspondence to S. Ramesh.

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Communicated by Izabela Pawłowicz.

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Sunitha, N.C., Gangappa, E., Gowda, R.P.V. et al. Discovery of genomic regions associated with resistance to late wilt disease caused by Harpophora maydis (Samra, Sabet and Hing) in maize (Zea mays L.). J Appl Genetics 63, 185–197 (2022). https://doi.org/10.1007/s13353-021-00672-x

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