According to the World Health Organization, bacterium Acinetobacter baumannii is the first on the critical priority list of pathogens in urgent need for new antibiotics. The increasing resistance of A. baumannii to the last-line treatment options, including carbapenems, is a global problem. We report the molecular epidemiology of 12 carbapenem-resistant clinical isolates of A. baumannii collected from hospitalised patients in three neighbouring countries in Southeast Europe: Croatia, Serbia, and Bosnia and Herzegovina, giving an insight into the molecular characterisation and evolutionary history of the acquisition of resistance genes. Besides the blaOXA-23 gene, the endemic presence of OXA-72 oxacillinase of the same origin for more than a decade as the leading mechanism of carbapenem resistance in Southeast Europe was confirmed. To the best of our knowledge, this is the first paper that investigates and analyses the phylogenetic association of the most common mechanisms of resistance to carbapenems in clinical isolates of A. baumannii originating from three neighbouring countries in Southeast Europe.
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This research was supported by awards for excellence (institutional funding) of the University of Split School of Medicine and the Croatian Science Foundation (grant no. IP-2014-09-5656). The blaOXA-72 gene sequence determined in this study has been marked as Cro1 and deposited in GenBank under number MN366238. The blaOXA-23 gene sequences determined in this study has been marked as Cro2 and deposited in GenBank under number MT393582.
This article does not contain any studies with human participants or animals performed by any of the authors.
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The part of this investigation was presented as a poster on the 12th International Symposium on the Biology of Acinetobacter in September 2019, in Frankfurt, Germany.
Communicated by: Agnieszka Szalewska-Palasz
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Goic-Barisic, I., Kovacic, A., Medic, D. et al. Endemicity of OXA-23 and OXA-72 in clinical isolates of Acinetobacter baumannii from three neighbouring countries in Southeast Europe. J Appl Genetics 62, 353–359 (2021). https://doi.org/10.1007/s13353-021-00612-9
- Acinetobacter baumannii
- Endemic oxacillinase
- Bosnia and Herzegovina