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Journal of Applied Genetics

, Volume 60, Issue 3–4, pp 417–426 | Cite as

3′ end of eae gene-based fluorescence DNA nanosensor for detection of E. coli O157:H7

  • Farrokh KarimiEmail author
  • Nasrin Balazadeh
  • Bagher Eftekhari-Sis
Microbial Genetics • Original Paper
  • 33 Downloads

Abstract

Escherichia coli O157:H7 bacterium as a zoonotic pathogen is one of the most important causative agents of foodborne illnesses worldwide. Due to the serious concerns in public health and enormous economic losses in agriculture and food industry, it is very necessary to develop novel technology–based methods for sensitive and rapid detection of this bacterium in contaminated resources. In this study, a sensitive and selective fluorescence DNA nanosensing platform based on graphene oxide (GO) and the 3′ end of eae gene as specific sequence was developed for the detection of E. coli O157:H7. In this platform, fluorescence resonance energy transfer (FRET) process between GO- and FAM-labeled eae gene probe was used for the diagnosis of E. coli O157:H7. Following the immobilization of the eae gene probe on GO, fluorescence emission of FAM was quenched. In hybridization reaction, by adding the complementary DNA, fluorescence emission of FAM was significantly increased and recovered to 93%. The performance of sensor for detection of E. coli O157:H7 genomic DNA was determined 10 pg genomic DNA per 1 ml Tris-HCl hybridization buffer which was significantly more sensitive than PCR method. In conclusion, the results indicated that GO eae gene-based nanosensor has potential to be developed as a rapid and sensitive diagnostic device besides PCR methods for the detection of E. coli O157:H7 bacteria.

Keywords

Genosensor E. coli O157:H7 Carbon nanomaterial Graphene oxide 

Notes

Funding information

This work was supported by funds from the research council of the University of Maragheh.

Compliance with ethical standards

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2019

Authors and Affiliations

  • Farrokh Karimi
    • 1
    Email author
  • Nasrin Balazadeh
    • 1
  • Bagher Eftekhari-Sis
    • 2
  1. 1.Department of Biotechnology, Faculty of ScienceUniversity of MaraghehMaraghehIran
  2. 2.Department of Chemistry, Faculty of ScienceUniversity of MaraghehMaraghehIran

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