Genetic analysis of drought response of wheat following either chemical desiccation or the use of a rain-out shelter

Tarawneh, Rasha A.; Szira, Fruzsina; Monostori, Istvan; Behrens, Annika; Alqudah, Ahmad M.; Thumm, Stefanie; Lohwasser, Ulrike; Röder, Marion S.; Börner, Andreas; Nagel, Manuela

doi:10.1007/s13353-019-00494-y

Plant Genetics • Original Paper
Published: 04 April 2019

Genetic analysis of drought response of wheat following either chemical desiccation or the use of a rain-out shelter

Rasha A. Tarawneh¹,
Fruzsina Szira²,
Istvan Monostori²,
Annika Behrens¹,
Ahmad M. Alqudah¹,
Stefanie Thumm¹,
Ulrike Lohwasser¹,
Marion S. Röder¹,
Andreas Börner¹ &
Manuela Nagel ORCID: orcid.org/0000-0003-0396-0333¹

Journal of Applied Genetics volume 60, pages 137–146 (2019)Cite this article

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Abstract

Simulating drought stress during the breeding process has been proposed as a way to select varieties under naturally non-stressful conditions. The aim of the study was to characterise the genetic basis of the response of 111 spring wheat (Triticum aestivum L.) varieties and landraces to chemical desiccation and to rain-out shelter drought. The effect of the rain-out shelter was a 15% reduction in plant height, spike length and thousand seed weight (TSW); in contrast, the desiccant treatment induced a 15% reduction in seed number, a 35–72% loss in TSW and a reduction in subsequent germination of 12%. A genome-wide association analysis revealed 263 significant marker-trait associations (MTAs), of which 246 involved days to anthesis, plant height, spike length, number of spikelets, seed number, TSW and germination from the non-treated plants. Only four and five MTAs involved TSW from plants grown under the rain-out shelter and the chemical desiccation, respectively, and harboured the Sugar-Dependent6 gene. Seven MTAs involved seed number for chemical desiccated plants. Both, chemical desiccation and rain-out shelter drought identified same tolerant genotypes. Concluding, both approaches are suitable to simulate different drought scenarios. However, there was a strong environmental impact for chemical desiccation which may increase the complexity of this tolerance mechanism.

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Acknowledgments

Anna Marthe, Allah Bakhsh, Sibylle Pistrick, Annett Marlow, Peter Schreiber, Philip Kouria and the past and current staff of the IPK gene bank as well as Freta K. Balladona and Farhadz Fadhillah Sandi are gratefully acknowledged for their technical support and Wolfgang Link for statistical advice.

Author contributions statement

MN, AB and UL designed the work; RT, MN, FS, IM, AMA, ST and MR acquired and analysed the data; RT and MN wrote the original draft of the manuscript and AMA, AB, RT and MN critically revised the content. All authors read and approved the manuscript.

Funding

This study was funded by the DAAD (Rasha Tarawneh).

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Affiliations

Genebank Department, Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben), Seeland, Germany
Rasha A. Tarawneh, Annika Behrens, Ahmad M. Alqudah, Stefanie Thumm, Ulrike Lohwasser, Marion S. Röder, Andreas Börner & Manuela Nagel
Hungarian Academy of Sciences, Agricultural Institute, Martonvásár, Hungary
Fruzsina Szira & Istvan Monostori

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Rasha A. Tarawneh
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Fruzsina Szira
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Istvan Monostori
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Annika Behrens
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Ahmad M. Alqudah
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Stefanie Thumm
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Ulrike Lohwasser
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Marion S. Röder
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Andreas Börner
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Manuela Nagel
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Correspondence to Manuela Nagel.

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Tarawneh, R.A., Szira, F., Monostori, I. et al. Genetic analysis of drought response of wheat following either chemical desiccation or the use of a rain-out shelter. J Appl Genetics 60, 137–146 (2019). https://doi.org/10.1007/s13353-019-00494-y

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Received: 28 June 2018
Revised: 16 March 2019
Accepted: 22 March 2019
Published: 04 April 2019
Issue Date: 01 May 2019
DOI: https://doi.org/10.1007/s13353-019-00494-y

Keywords

Abiotic stress
Drought simulation
Genetic mapping
GWAS
Phenotyping