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Molecular characterisation of tumour necrosis factor alpha and its potential connection with lipoprotein lipase and peroxisome proliferator-activated receptors in blunt snout bream (Megalobrama amblycephala)

  • Animal Genetics • Original Paper
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Abstract

Tumour necrosis factor alpha (TNF-α) is one kind of cytokines which is related to inflammation and lipid metabolism. TNF-α cDNA was cloned from the liver of blunt snout bream (Megalobrama amblycephala) through real-time polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) methods. The full-length cDNA of TNF-α covered 1467 bp, with an open reading frame (ORF) of 723 bp, which encodes 240 amino acids. It possessed the TNF family signature IIIPDDGIYFVYSQ. After the lipopolysaccharide (LPS) challenge test, a graded tissue-specific expression pattern of TNF-α was observed and there was high expression abundance in the kidney, brain and liver. After 8 weeks feeding trial, liver samples, two groups fed with 6% and 11% lipid levels, were collected. The results showed that, for fish fed with high-fat diet, the triglyceride of serum and lipid content of liver were elevated. Furthermore, TNF-α and peroxisome proliferator-activated receptors (PPARα, β) mRNA expression of fish fed 11% lipid diet were significantly up-regulated (p < 0.05). Lipoprotein lipase (LPL) and PPARγ mRNA expression of fish fed 11% lipid lever diet were significantly decreased compared to those of fish fed 6% (p < 0.05). The differences between the various expression of related genes in the high and low fat groups demonstrated that TNF-α played a key role in lipid metabolism, which may have an influence on fat metabolism through reducing fat synthesis and strengthening the β-oxidation of fatty acid. These discrepancies warrant further research.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31502178), the Fundamental Research Funds for the Central Universities (KJQN201612) and the National Technology System for Conventional Freshwater Fish Industries of China (CARS-46-20).

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Authors and Affiliations

  1. Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Man Zhou, Wen-Bin Liu, Kai-Zhou Wang & Guang-Zhen Jiang

  2. Tongwei Company Limited, No. 588 Middle Section of Tianfu Avenue, Chengdu Hi-Tech Zone, Chengdu, 610041, People’s Republic of China

    Hai-Feng Mi & Ye-Yang Wu

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  1. Man Zhou
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Correspondence to Guang-Zhen Jiang.

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Funding

This study was funded by the National Natural Science Foundation of China (31502178), the Fundamental Research Funds for the Central Universities (KJQN201612) and the National Technology System for Conventional Freshwater Fish Industries of China (CARS-46-20). Guang-Zhen Jiang has received research grants from the National Natural Science Foundation of China (31502178) and the Fundamental Research Funds for the Central Universities (KJQN201612). Wen-Bin Liu is a member of the National Technology System for Conventional Freshwater Fish Industries of China.

Conflict of interest

All authors in this experiment declare no conflict of interest.

Ethical approval

Animal care and use were conducted in accordance with the Animal Research Institute Committee guidelines of Nanjing Agriculture University, China. This study was specifically approved by the Committee of the Animal Research Institute of Nanjing Agriculture University, China.

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Communicated by: Maciej Szydlowski

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Zhou, M., Mi, HF., Liu, WB. et al. Molecular characterisation of tumour necrosis factor alpha and its potential connection with lipoprotein lipase and peroxisome proliferator-activated receptors in blunt snout bream (Megalobrama amblycephala). J Appl Genetics 58, 381–391 (2017). https://doi.org/10.1007/s13353-017-0390-9

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  • DOI: https://doi.org/10.1007/s13353-017-0390-9

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