Abstract
NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles. However, similar research on the bovine NOTCH1 gene is lacking. In this study, we detected the polymorphisms of the bovine NOTCH1 gene in a total of 448 individuals from Chinese Qinchuan cattle with DNA pooling, forced PCR-RFLP, and DNA sequencing methods. Five novel SNPs were identified within the NICD domain, and eight haplotypes comprising combinations of these five SNPs were studied as well. The association analysis of SNPs’ effects with growth traits revealed that g.A48250G was significantly associated with body height, body weight, and height at hip cross, and that g.A49239C only showed significant associations with body height. This suggests that the NOTCH1 gene is a strong candidate gene that could be utilized as a promising marker in beef cattle breeding programs.
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Acknowledgments
This study was supported by the Program of National Beef Cattle and Yak Industrial Technology System (CARS-38), the National Natural Science Foundation of China (no. 31272408), the National 863 Program of China (Grant No. 2013AA102505), and Science and Technology Co-ordinator Innovative engineering projects of Shaanxi Province (No. 2014KTZB02-02-02-02, No. 2015KTCL02-08), Project of breeding and application of Pinan Cattle.
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Communicated by: Maciej Szydlowski
Mei Liu and Chenge Zhang contributed equally to this work.
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Liu, M., Zhang, C., Lai, X. et al. Associations between polymorphisms in the NICD domain of bovine NOTCH1 gene and growth traits in Chinese Qinchuan cattle. J Appl Genetics 58, 241–247 (2017). https://doi.org/10.1007/s13353-016-0377-y
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DOI: https://doi.org/10.1007/s13353-016-0377-y