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Effect of mevalonic acid on cholesterol synthesis in bovine intramuscular and subcutaneous adipocytes

  • Animal Genetics • Original Paper
  • Published:
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Abstract

Mevalonic acid (MVA) is a key material in the synthesis of cholesterol; indeed, intracellular cholesterol synthesis is also called the mevalonic acid pathway. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is an essential enzyme in cholesterol biosynthesis. This study suggests that MVA may play an important role in the differentiation of bovine adipose tissue in vivo. We investigated differential mRNA expression in bovine intramuscular preadipocytes (BIPs) and bovine subcutaneous preadipocytes (BSPs) by culturing cells from the longissimus dorsi muscle and subcutaneous fat tissues of Luxi yellow cattle. The morphology of lipid accumulation of bovine preadipocytes was detected by Oil Red O staining, and total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), and high-density lipoprotein cholesterol (HDLC) levels were measured. Temporospatial expression of HMGR was investigated by real-time quantitative polymerase chain reaction (PCR). The TC, LDLC, and HDLC content did not significantly differ over time but increased slowly with increasing MVA concentration. HMGR expression increased over time and with increasing concentrations of MVA. MVA increased adipose cell proliferation in a dose-dependent and time-dependent manner. MVA stimulated HMGR expression in two cell types and its influence on adipocyte differentiation.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31100890) and the Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (BS2010SW026).

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    Authors and Affiliations

    1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji’nan City, Shandong Province, 250100, China

      Xiaomu Liu, Wei You, Haijian Cheng, Enliang Song, Fachun Wan, Hong Han & Guifen Liu

    2. Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China

      Xiaomu Liu, Wei You, Haijian Cheng, Enliang Song, Fachun Wan, Hong Han & Guifen Liu

    3. Department of Animal Science, Heze University, Heze, 274015, China

      Qingfeng Zhang

    Authors
    1. Xiaomu Liu
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    2. Wei You
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    3. Haijian Cheng
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    4. Qingfeng Zhang
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    5. Enliang Song
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    Corresponding author

    Correspondence to Guifen Liu.

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    Communicated by: Maciej Szydlowski

    Highlights

    We evaluated the effects of MVA on cholesterol content and gene expression in vitro.

    Temporospatial expression of HMGR was investigated by real-time quantitative PCR after treatment with MVA.

    MVA stimulated HMGR expression in intramuscular and subcutaneous adipocytes.

    Xiaomu Liu and Wei You contributed equally to this work.

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    Liu, X., You, W., Cheng, H. et al. Effect of mevalonic acid on cholesterol synthesis in bovine intramuscular and subcutaneous adipocytes. J Appl Genetics 57, 113–118 (2016). https://doi.org/10.1007/s13353-015-0300-y

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    • DOI: https://doi.org/10.1007/s13353-015-0300-y

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