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Chromosomal manipulation in Senegalese sole (Solea senegalensis Kaup, 1858): induction of triploidy and gynogenesis

  • Animal Genetics • Original Paper
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Abstract

In this study we have developed protocols for induced triploidy and gynogenesis of Senegalese sole (Solea senegalensis), a promising flatfish species for marine aquaculture, in order to: 1) identify the sex-determination mechanism; and 2) to improve its production by generating a) sterile fish, avoiding problems related with sexual maturation, and b) all-female stocks, of higher growth rate. Triploidy was induced by means of a cold shock. Gynogenesis was induced by activating eggs with UV-irradiated sperm, and to prompt diploid gynogenesis, a cold-shock step was also used. Ploidy of putative triploid larvae and gynogenetic embryos were determined by means of karyotyping and microsatellite analysis. Haploid gynogenetic embryos showed the typical “haploid syndrome”. As expected, triploid and gynogenetic groups showed lower fertilization, hatching, and survival rates than in the diploid control group. Survival rate, calculated 49 days after hatching, for haploid and diploid gynogenetic groups was similar to those observed in other fish species (0 % and 62.5 %, respectively), whereas triploids showed worse values (45 %). Sex was determined macroscopically and by histological procedures, revealing that all the diploid gynogenetic individuals were females. In conclusion, we have successfully applied chromosomal-manipulation techniques in the flatfish species Senegalese sole in order to produce triploid, haploid, and diploid gynogenetic progenies.

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Acknowledgments

This work has been supported by the Spanish Ministry of Science and Innovation (AGL2009-11872), Pleurogene-Flatfish Genomics and the Consolider-Ingenio AQUAGENOMICS Project (CSD2007-00002).

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Authors and Affiliations

  1. Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain

    Maria Jesús Molina-Luzón, Francisca Robles, Rafael Navajas-Pérez, Carmelo Ruiz-Rejón & Roberto De la Herrán

  2. IFAPA, Centro Agua del Pino, Consejería de Agricultura y Pesca, Junta de Andalucía, 21450, Cartaya, Huelva, Spain

    Jose Ramón López & Jose Ignacio Navas

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  1. Maria Jesús Molina-Luzón
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  2. Jose Ramón López
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  3. Francisca Robles
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  4. Rafael Navajas-Pérez
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  5. Carmelo Ruiz-Rejón
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  6. Roberto De la Herrán
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  7. Jose Ignacio Navas
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Correspondence to Maria Jesús Molina-Luzón.

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Molina-Luzón, M.J., López, J.R., Robles, F. et al. Chromosomal manipulation in Senegalese sole (Solea senegalensis Kaup, 1858): induction of triploidy and gynogenesis. J Appl Genetics 56, 77–84 (2015). https://doi.org/10.1007/s13353-014-0233-x

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  • DOI: https://doi.org/10.1007/s13353-014-0233-x

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