Journal of Applied Genetics

, Volume 55, Issue 3, pp 417–422 | Cite as

Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran

  • Mohammad KargarEmail author
  • Sadegh Ghorbani-Dalini
  • Abbas Doosti
  • Akram Najafi
Microbial Genetics • Original Paper


CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine phosphorylation motifs (TPMs) of H. pylori. A set of 302 H. pylori DNA samples from the Iranian population from 2006 to 2011 was selected for the proposed study. The cagA gene and its TPMs were assessed by using polymerase chain reaction (PCR) and specific primers. The prevalence of the cagA gene in our study ranged from 91.43 % to 97.06 % (with an average of 95.03 %). Out of the cagA-positive samples, the prevalence of TPMs A and B increased from 12.5 % and 23.44 % to 71.2 % and 63.63 %, respectively. Also, the prevalence of samples infected with Western and East Asian types of H. pylori ranged from 64.06 % to 5.73 % for the Western type and 17.19 % to 51.59 % for the East Asian type. Overall, our results showed a high prevalence of the cagA gene. Also, it seems that cagA TPMs of H. pylori is undergoing a change from the Western type to the East Asian type in Iran.


Helicobacter pylori cagA TPMs Iran 



All the authors are grateful to the Jahrom and Shahrekord Branches of the Islamic Azad University, and the staff of the Biotechnology Research Center, for their executive support of this project.


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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2014

Authors and Affiliations

  • Mohammad Kargar
    • 1
    Email author
  • Sadegh Ghorbani-Dalini
    • 2
  • Abbas Doosti
    • 3
  • Akram Najafi
    • 4
  1. 1.Department of Microbiology, Jahrom BranchIslamic Azad UniversityJahromIran
  2. 2.Department of Microbiology, Jahrom Branch, Young Researcher’s ClubIslamic Azad UniversityJahromIran
  3. 3.Biotechnology Research Center, Shahrekord BranchIslamic Azad UniversityShahrekordIran
  4. 4.Department of Marine Microbiology, The Persian Gulf Marine Biotechnology Research CenterBushehr University of Medical SciencesBushehrIran

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