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Induction of meiotic gynogenesis in ship sturgeon Acipenser nudiventris using UV-irradiated heterologous sperm

  • Animal Genetics • Original Paper
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Abstract

Diploid gynogenesis was induced in ship sturgeon Acipenser nudiventris using UV-irradiated sperm from Siberian sturgeon Acipenser baerii. The optimal condition for the retention of the second polar body in ship sturgeon was determined to be 10 min after activation/fertilization in experiments. The temperature of cold shock and its duration were 2.5 °C and 30 min, respectively. A total of 30 gynogens of known parentage from experimental treatments were screened using microsatellite DNA analysis, and uniparental transmission in meiogens was confirmed. The results show that heterologous Siberian sturgeon sperm is applicable as UV-irradiated sperm for the induction of gynogenesis in ship sturgeon. This technique may recover the critically endangered sturgeon species that are becoming extinct.

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Acknowledgments

The study was financially supported by the Iranian Fisheries Research Organization. We thank Mr. Houshang Yeganeh for his help with the fish culture, Mr. Masoud Alizadeh for providing live food, and Mr. Hosein Mohammadi Parashkooh for providing the hatchery facilities required for this study.

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Authors and Affiliations

  1. Genetics Department, International Sturgeon Research Institute, P.O. Box 41635-3464, Rasht, Iran

    Mohammad Hassanzadeh Saber, Shahrouz Baradaran Noveiri, Mohammad Pourkazemi, Mohammadali Yazdani, Mahmoud Bahmani, Mohammad Pourdehghani, Mahtab Yarmohammadi & Mohammadreza Nowruzfashkhami

  2. Iranian Fisheries Research Organization, P.O. Box 14155-6116, Tehran, Iran

    Ahmad Ghoroghi

  3. Aquaculture & Breeding Department, Inland Water Aquaculture Institute, P.O. Box 66, Bandar Anzali, Iran

    Fereidoon Chakmehdouz

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  1. Mohammad Hassanzadeh Saber
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  2. Shahrouz Baradaran Noveiri
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  3. Mohammad Pourkazemi
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  4. Mohammadali Yazdani
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  5. Ahmad Ghoroghi
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  6. Mahmoud Bahmani
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  7. Mohammad Pourdehghani
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  8. Fereidoon Chakmehdouz
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  9. Mahtab Yarmohammadi
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  10. Mohammadreza Nowruzfashkhami
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Correspondence to Mohammad Hassanzadeh Saber.

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Hassanzadeh Saber, M., Noveiri, S.B., Pourkazemi, M. et al. Induction of meiotic gynogenesis in ship sturgeon Acipenser nudiventris using UV-irradiated heterologous sperm. J Appl Genetics 55, 223–229 (2014). https://doi.org/10.1007/s13353-013-0188-3

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  • DOI: https://doi.org/10.1007/s13353-013-0188-3

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