Abstract
In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.
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Acknowledgment
This work was supported by the Ministry of Agriculture of the Czech Republic (project MZE 0002716202), the Grant Agency of the Czech Republic (grant P506/10/0421), and by the project “CEITEC—Central European Institute of Technology” (CZ.1.05/1.1.00/02.0068) from the European Regional Development Fund (ERDF). The authors wish to thank Mr. Paul Veater (Bristol, United Kingdom) for proofreading the translated manuscript.
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Cabelova, K., Kubickova, S., Cernohorska, H. et al. Male-specific repeats in wild Bovidae. J Appl Genetics 53, 423–433 (2012). https://doi.org/10.1007/s13353-012-0108-y
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DOI: https://doi.org/10.1007/s13353-012-0108-y