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A coupled bimodal SPECT-CT imaging and brain kinetics studies of zolmitriptan-encapsulated nanostructured polymeric carriers

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Abstract

The present investigation deals with preparation and characterization of anti-migraine zolmitriptan (ZMT) nanostructured polymeric carriers for nose to brain drug targeting. The drug-loaded colloidal nanocarriers of ZMT were prepared by modified ionic gelation of cationic chitosan with anionic sodium tripolyphosphate and characterized for particle size, zeta potential, and entrapment efficiency. Further, in order to investigate nose to brain drug targeting, biodistribution, and brain kinetics studies were performed using 99mtechnetium radiolabeled nanocarriers (99mTc-ZMTNP) in Swiss albino mice. The results were compared with intranasal pure drug solution (99mTc-ZMT) and intravenous nanocarriers (99mTc-ZMTNP). A single photon emission computerized tomography (SPECT) radioimaging studies were also carried out to visualize and confirm brain uptake of nanocarriers. The optimized nanocarriers showed particle size of 161 nm, entrapment efficiency of 80.6%, and zeta potential of + 23.7 mV. The pharmacokinetic parameters, Cmax, and AUC0-∞ values for ZMT concentration in the brain expressed as percent radioactivity per gram of brain in intranasal and intravenous route of administration were calculated. The brain Cmax and AUC0-∞ values found in three groups, intranasal 99mTc-ZMTNP, intranasal 99mTc-ZMT, and intravenous 99mTc-ZMTNP were (0.427 and 1.889), (0.272 and 0.7157), and (0.204 and 0.9333), respectively. The higher Cmax values of intranasal 99mTc-ZMTNP suggests better brain uptake as compared to other routes of administration. The significant higher values of nose to brain targeting parameters namely, drug targeting index (5.57), drug targeting efficiency (557.08%), and nose to brain drug direct transport (82.05%) confirmed drug targeting to brain via nasal route. The coupled bimodal SPECT-CT scintigrams confirm the brain uptake of intranasal 99mTc-ZMTNP demonstrating major radioactivity accumulation in brain. This study conclusively demonstrated the greater uptake of ZMT-loaded nanocarriers by nose to brain drug targeting, which proves promising drug delivery system.

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Acknowledgements

The authors are grateful to Cipla Ltd., Mumbai, India, for providing a gift sample of ZMT. Authors are thankful to Savitribai Phule Pune University, India for providing financial support in the form of a research grant.

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Authors and Affiliations

  1. Department of Pharmaceutics, Sinhgad Technical Education Society’s, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune, Maharashtra, India

    Satish K. Mandlik & Nisharani S. Ranpise

  2. Department of Pharmaceutics, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India

    Satish K. Mandlik

  3. Small Animal Imaging Facility, ACTREC, Navi Mumbai, Maharashtra, India

    Bhabani S. Mohanty & Pradip R. Chaudhari

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Correspondence to Satish K. Mandlik.

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Mandlik, S.K., Ranpise, N.S., Mohanty, B.S. et al. A coupled bimodal SPECT-CT imaging and brain kinetics studies of zolmitriptan-encapsulated nanostructured polymeric carriers. Drug Deliv. and Transl. Res. 8, 797–805 (2018). https://doi.org/10.1007/s13346-017-0474-4

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  • DOI: https://doi.org/10.1007/s13346-017-0474-4

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