Skip to main content
Log in

High positivity rate of caprine arthritis encephalitis virus in Luzon, the Philippines revealed by nested-polymerase chain reaction assay

  • Original Article
  • Published:
VirusDisease Aims and scope Submit manuscript

Abstract

Caprine arthritis encephalitis (CAE) is a worldwide economically important disease of small ruminants particularly goats. CAE has been considered to be an emerging/re-emerging disease of goats and a notifiable disease in the Philippines. In this study, a nested-PCR method to detect CAE virus (CAEv) infection was conducted between January 2021 to December 2022. A total of 1334 goat blood samples were collected from 24 goat farms throughout Luzon, the Philippines. The over-all prevalence rate was 31.41% (419/1334) in goats and 91.67% (22/24) of goat farms. These results showed high positivity rate of CAEv and the disease may be widespread in Luzon, the Philippines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  1. Adams DS, Klevjer-Anderson P, Carlson JL, McGuire TC, Gorham JR. Transmission and control of caprine arthritis-encephalitis virus. Am J Vet Res. 1983;44(9):1670–5.

    CAS  PubMed  Google Scholar 

  2. Al Ahmad MZA, Dubreil L, Chatagnon G, Khayli Z, Theret M, Martignat L, Chebloune Y, Fieni F. Goat uterine epithelial cells are susceptible to infection with caprine arthritis encephalitis virus (CAEV) in vivo. Vet Res. 2012;43(1):1–7. https://doi.org/10.1186/1297-9716-43-5.

    Article  CAS  Google Scholar 

  3. Andres D, Klein D, Watt NJ, Berritua E, Torteinsdottir S, Blacklaws BA, Harkiss GD. Diagnostic tests for small ruminant lentiviruses. Vet Microbiol. 2005;107(1–2):49–62. https://doi.org/10.1016/j.vetmic.2005.01.012.

    Article  CAS  PubMed  Google Scholar 

  4. BAI. Handbook on diagnostic laboratory data 2004. Philippine Animal Health Center, Bureau of Animal Industry. Department of Agriculture, 2004.

  5. Balbin MM, Mingala CN. Caprine arthritis-encephalitis. In: Bayry J, editor. Emerging and Re-emerging infectious diseases of livestock. Cham: Springer Nature; 2017. p. 191–213.

    Chapter  Google Scholar 

  6. Bandeira DA, de Castro RS, Azevedo EO, de Souza Seixas Melo L, de Melo CB. Seroprevalence of caprine-arthritis encephalitis virus infection in goats in the Cariri region, Paraiba State, Brazil. Vet J. 2009;180(3):399–401. https://doi.org/10.1016/j.tvjl.2008.02.007.

    Article  PubMed  Google Scholar 

  7. Bartak P, Simek B, Vaclavek P, Curn V, Plodkova H, Tonka T, Farkova B, Vernerova K, Vejcik A. A Genetic characterization of small ruminant lentiviruses in sheep and goats from the Czech Republic. Acta Vet Brno. 2018;87(1):19–26. https://doi.org/10.2754/avb201887010019.

    Article  Google Scholar 

  8. Cana A, Taylor N, Honhold N, Gjinovci V, Osmani A, Nardelli S, Murati B, Spahiu J, Mehmetukaj D, Alishani M. Sero-prevalence survey of small ruminant lentivirus (SRLV) infections in Kosovo. Kafkas Univ Vet Fak Derg. 2020;26(1):1–8.

    Google Scholar 

  9. Crawford TB, Adams DS, Cheevers WP, Cork LC. Chronic arthritis in goats caused by retrovirus. Science. 1980;207(4434):997–9. https://doi.org/10.1126/science.6153243.

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Enache DA, Baraitareanu S, Danes D. The incidence of SRLVs infection in European countries over the 10 year period, 2006 to 2015. Agric Food. 2016;4(1):518–29.

    Google Scholar 

  11. Gonzales JCV, Domingo CYJ, Abes NS, Gutierrez CA, Villanueva MA, Mingala CN. Concordance of competitive enzyme linked immunosorbent assay and nested-polymerase chain reaction in the detection of caprine arthritis-encephalitis virus. Small Rumin Res. 2013;115(1–3):134–9. https://doi.org/10.1016/j.smallrumres.2013.08.005.

    Article  Google Scholar 

  12. Gonzales B, Reina R, Garcia L, Andres S, Glaria I, Alzueta M, Mora MI, Jugo BM, Arrieta-Aguirre I, de la Lastra JM, Rodríguez D, Rodríguez JR, Esteban M, Grilló MJ, Blacklaws BA, Harkiss GD, Chebloune Y, Luján L, de Andrés D, Amorena B. Mucosal immunization of sheep with a maedi-visna virus (MVV) env DNA vaccine against protects against early MVV productive infection. Vaccine. 2005;23(34):4342–52. https://doi.org/10.1016/j.vaccine.2005.03.032.

    Article  CAS  Google Scholar 

  13. Grewal AS, Greenwood PE, Burton RW, Smith JE, Batty EM, North R. Caprine retrovirus infection in New South Wales: virus isolations, clinical and histopathological findings and prevalence of antibody. Aust Vet J. 1986;63(8):245–8. https://doi.org/10.1111/j.1751-0813.1986.tb02985.x.

    Article  CAS  PubMed  Google Scholar 

  14. Gufler H, Baumgartner W. Overview of herd and CAEV status in dwarf goats in South Tyrol. Italy Vet Q. 2007;29(2):68–70. https://doi.org/10.1080/01652176.2007.9695229.

    Article  CAS  PubMed  Google Scholar 

  15. Herrera PCT, Viloria VV, Balbin MM, Mingala CN. Prevalence of babesiosis (Babesia bovis and Babesia bigemina) in cattle and water buffalo in Nueva Ecija Philippines using nested polymerase chain reaction. Ann Parasitol. 2017;63(4):309–16. https://doi.org/10.17420/ap6304.117.

    Article  PubMed  Google Scholar 

  16. Idres T, Lamara A, Temim S, Boudjellaba S, Gagnon J, Chebloune Y. Serological diagnosis of lentivirus infection in goats raised in Algeria. J Vet Res. 2019;63(1):27–33. https://doi.org/10.2478/jvetres-2019-0001.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Kojouri GA, Emami M, Momtaz H. The first molecular detection of caprine arthritis encephalitis virus (CAEV) in Iran. J Vet Sci Technol. 2014;5(3):1. https://doi.org/10.4172/2157-7579.1000184.

    Article  Google Scholar 

  18. Konishi M, Tsuduku S, Haritani M, Murakami K, Tsuboi T, Kobayashi C, Yoshikawa K, Kimura K, Sentsui H. An epidemic of caprine arthritis encephalitis in Japan: isolation of the virus. J Vet Med Sci. 2004;66(8):911–7. https://doi.org/10.1292/jvms.66.911.

    Article  PubMed  Google Scholar 

  19. Lara M, Birgel EJR, Birgel E. Possibility of vertical transmission of caprine arthritis encephalitis virus in neonate kids. Arq Bras Med Vet Zootec. 2005;57:553–5.

    Article  Google Scholar 

  20. Lin TN, Ngarmkun S, Oraveerakul K, Virakul P, Techakumphu M. Seroprevalence and risk-factors associated with caprine arthritis-encephalitis virus in goats in the western part of Thailand. Thai J Vet Med. 2011;41(3):353–60. https://doi.org/10.56808/2985-1130.2320.

    Article  Google Scholar 

  21. Lluz AT, Orais AA, Dumilon RA, Orais AT, Cerbito WA. First detection of caprine arthritis encephalitis infection in goats using ELISA test in Eastern Visayas, Philippines. Phillipp J Vet Anim Sci. 2011;37(2):159–66.

    Google Scholar 

  22. De la Luz-Armendariz J, Ducoing-Watty AE, Ramirez-Mendoza H, Gomez-Nuñez L, Tufiño-Loza C, Cabrera-Dominguez EM, Diaz-Aparicio E, Rivera-Benitez JF. Prevalence, molecular detection, and pathological characterization of small ruminant lentiviruses in goats from Mexico. Small Rumin Res. 2021;202:106474. https://doi.org/10.1016/j.smallrumres.2021.106474.

    Article  Google Scholar 

  23. Mosa AH, Hamzah KJ, Aljabory HAH. First study of the molecular prevalence of caprine arthritis encephalitis virus in goats in Babylon, Iraq. Vet World. 2022;15(4):1129–33. https://doi.org/10.14202/vetworld.2022.1129-1133.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Padiernos RBC, Balbin MM, Parayao AM, Mingala CN. Molecular characterization of the gag gene of caprine arthritis encephalitis virus from goats in the Philippines. Arch Virol. 2015;160(4):969–78. https://doi.org/10.1007/s00705-015-2359-5.

    Article  CAS  PubMed  Google Scholar 

  25. Peterhans E, Greenland T, Badiola J, Harkiss G, Bertoni G, Amorena B, Eliaszewicz M, Juste RA, Krassnig R, Lafont JP, Lenihan P, Petursson G, Pritchard G, Thorley J, Vitu C, Mornex JF, Pepin M. Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication scheme. Vet Res. 2004;35(3):257–74. https://doi.org/10.1051/vetres:2004014.

    Article  PubMed  Google Scholar 

  26. Peterson K, Brinkhof J, Houwers D, Colenbrander B, Gadella B. Presence of pro-lentiviral DNA in male sexual organ and ejaculates of small ruminants. Theriogeniology. 2008;69:433–42. https://doi.org/10.1016/j.theriogenology.2007.10.013.

    Article  CAS  Google Scholar 

  27. PSA. Goat Situation Report. Philippine statistics authority. https://psa.gov.ph/livestock-poultry-iprs/goat/inventory; 2023.

  28. Pugh DG. Sheep and goat medicine. W. B. Saunders Company, Philadelphia, pp. 468, 2002.

  29. Ravazzolo AP, Nenci C, Vogt H, Waldvogel A, Obexer-Ruff G, Peterhans E, Bertoni G. Viral load, organ diffusion, histopathological lesions, and cytokine mRNA expression in goats infected with a molecular clone of the caprine arthritis encephalitis virus. Virology. 2006;350:116–27. https://doi.org/10.1016/j.virol.2006.02.014.

    Article  CAS  PubMed  Google Scholar 

  30. Rimstad E, East NE, Derock E, Higgins MJ, Pedersen NC. Detection of antibodies to caprine arthritis-encephalitis virus using recombinant gag proteins. Arch Virol. 1994;134(3–4):345–56. https://doi.org/10.1007/BF01310572.

    Article  CAS  PubMed  Google Scholar 

  31. Rowe JD, East NE, Franti CE, Thurmond MC, Pedersen NC, Theilen GH. Risk factors associated with the incidence of seroconversion to caprine arthritis-encephalitis virus in goats on California dairies. Am J Vet Res. 1992;53(12):2396–403.

    Article  CAS  PubMed  Google Scholar 

  32. Shuralev EA, Khammadov NI, Osyanin KA, Elizarova IA, Salmanova GR, Shamaev ND, Petrov SV, Whelan C, Saushkin NY, Samsanova JV, Galimzyanov IG, Efimova MA, Khaertynov KS, Faizov TK, Mukminov MK, Ivanov AV. Initial multi-target approach shows importance of caprine arthritis encephalitis virus control program in Russia for hobbyist goat farms. Vet World. 2021;14(7):1718–26. https://doi.org/10.14202/vetworld.2021.1718-1726.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Smith MC, Sherman DM. Goat medicine. 2nd ed. Iowa: Wiley-Blackwell; 2009. p. 620.

    Book  Google Scholar 

  34. Taskaya H, Kale M. Investigation of caprine arthritis encephalitis virus (CAEV) infection in Honamli goat breed. MAE Vet Fak Derg. 2020;5(2):58–63. https://doi.org/10.24880/maeuvfd.682590.

    Article  Google Scholar 

  35. Wolf C. Update on small ruminant lentiviruses. Vet Clin Food Anim. 2021;37(1):199–208. https://doi.org/10.1016/j.cvfa.2020.12.003.

    Article  Google Scholar 

  36. Yang WC, Chen HY, Wang CY, Pan HY, Wu CW, Hsu YH, Su JC, Chan KW. High prevalence of caprine arthritis encephalitis virus (CAEV) in Taiwan revealed by large-scale serological survey. J Vet Med Sci. 2017;79(2):273–6. https://doi.org/10.1292/jvms.16-0387.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank the Philippine Carabao Center for the support to finish this study. In addition, special thanks to all the staff of Biosafety and Environment Section and Livestock Biotechnology Center for their technical support.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Claro N. Mingala.

Ethics declarations

Conflict of interest

The authors report no declarations of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tubalinal, G.A.S.P., Belotindos, L.P. & Mingala, C.N. High positivity rate of caprine arthritis encephalitis virus in Luzon, the Philippines revealed by nested-polymerase chain reaction assay. VirusDis. (2024). https://doi.org/10.1007/s13337-023-00856-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13337-023-00856-5

Keywords

Navigation