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Identification of SSR markers through bulk segregant analysis and inheritance of resistance to yellow vein mosaic disease in okra (Abelmoschus esculentus L. Moench)

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Abstract

Okra is an important traditional vegetable crop grown for its tender fruits in various tropical and sub tropical parts of the world. Yellow Vein Mosaic Disease (YVMD) is the major biotic factor causing severe threat to the okra fruit yield and qualities. The present study was conducted to find out the inheritance of resistance against YVMD and to identify the disease linked molecular markers through bulk segregant analysis. For this, the F1, BC1F1 and BC1F2 generations were derived from a cross between Abelmoschus manihot (PAUAcc-1) as resistant male parent and A. esculentus cv. Punjab Padmini as susceptible female parent. The whole set of populations (F1, BC1F1 and BC1F2) along with parents were subjected to artificial as well as filed screening against YVMD. Chi-square test for goodness to fit revealed that resistance against YVMD is controlled by two recessive genes. The allele of at least one gene in homozygous state mask the effect of other gene and produce a resistant phenotype. The very low polymorphism (31.5%) was detected between the parents by using SSR primers. Out of 200 SSR primers, the four primers i.e. Okra 032, Okra 049, Okra 129 and Okra 270 were found to be linked to YVMD through bulk segregant analysis. The identified SSR primers to YVMD could be further used in okra improvement for YVMD resistance.

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Acknowledgements

The Authors are grateful to the authorities of Punjab Agricultural University, Ludhiana for providing the required resources and support for the conduct of this research work. The first author is the student of Mamta Pathak, for his Ph. D thesis submitted to Punjab Agricultural University, Ludhiana.

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  1. Punjab Agricultural University, Ludhiana, Punjab, India

    Gagandeep Singh, Mamta Pathak, Dharminder Pathak & Navraj Kaur Sarao

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  1. Gagandeep Singh
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Correspondence to Gagandeep Singh.

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Singh, G., Pathak, M., Pathak, D. et al. Identification of SSR markers through bulk segregant analysis and inheritance of resistance to yellow vein mosaic disease in okra (Abelmoschus esculentus L. Moench). VirusDis. 34, 498–503 (2023). https://doi.org/10.1007/s13337-023-00844-9

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