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Transcriptome sequencing analysis of porcine alveolar macrophages infected with PRRSV strains to elucidate virus pathogenicity and immune evasion strategies

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Abstract

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) causes a serious disease to the swine industry worldwide. To understand the mechanisms of HP-PRRSV infection, RNA-seq-based transcriptome analyses were performed on porcine alveolar macrophages (PAMs) infected with a HP-PRRSV strain (TJ), a less virulent strain of a classical lineage (CH-1a), and a vaccine strain TJM-F92. Gene ontology, Kyoto Encyclopedia of Genes and Genomes analyses indicate that TJM-F92 led to significant up-regulation of gene expression for proteins associated with membrane-bound organelles. The differentially expressed genes of HP-PRRSV TJ-infected PAM cells were up-regulated in the special G-protein coupled receptor. The six cytokines were tested by real time Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The relative expression levels showed the same trend of expression difference. Significant up-regulation of TMEM173 plays an important role in the cytosolic DNA-sensing pathway and the RIG-I-like receptor signaling pathway in TJM-F92 infected PAM cells. These data provide new insight into PRRSV pathogenicity and immune evasion strategies.

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Acknowledgements

This work was funded by High-level Talents Introduction Project of Inner Mongolia Agricultural University (NDYB2018-2), the Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University ‘Research on prevention and control of herbivore animal diseases and biological products’ (NDGCC2016-22), and National Natural Science Foundation of China (NSFC)‘Molecular mechanism for synaptic recycling dysfunction induced by G protein of street rabies virus in primary mouse neural cells’(31572505). The study was also supported with Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, P.R China and Ruminant Animal Disease Diagnosis center, Inner Mongolia Agricultural University. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript

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Authors and Affiliations

  1. College of Veterinary Medicine, Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, Inner Mongolia Agricultural University, No. 29, E’erduosi east street, Saihan Region, Hohhot, People’s Republic of China

    Feng-Xue Wang & Yong-Jun Wen

  2. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jingyue Economic and Technological Development Zone, No. 4899, Juye Avenue, Changchun, Jilin, 130112, People’s Republic of China

    Xing Liu & Yong-Jun Wen

  3. Sinovet (Beijing) Biotechnology Co., Ltd, B302 Zhongguancun Biomedical Park, 5 Shangdikaituo Road, Beijing, People’s Republic of China

    Hua Wu

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  1. Feng-Xue Wang
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Correspondence to Yong-Jun Wen.

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Wang, FX., Liu, X., Wu, H. et al. Transcriptome sequencing analysis of porcine alveolar macrophages infected with PRRSV strains to elucidate virus pathogenicity and immune evasion strategies. VirusDis. 32, 559–567 (2021). https://doi.org/10.1007/s13337-021-00724-0

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  • DOI: https://doi.org/10.1007/s13337-021-00724-0

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