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First detection of orchid fleck virus in orchids in Mexico

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Abstract

For the first time, an isolate of the dichorhavirus orchid fleck virus (OFV, family Rhabdoviridae) was found infecting an orchid plant in Mexico. The infected sample of Epidendrum veroscriptum was collected in a nursery in Lagunillas, municipality of Zihuateutla, Edo. Puebla. Mites gathered on this plant were analyzed by light and scanning electron microscopy, which consistently indicated the presence of adults of the species Brevipalpus californicus, the common vector of OFV. Viral identification was based on symptoms, cytopathology, and reverse transcriptase-PCR/sequencing of genome fragments of the RNA1 and 2 molecules. Since isolates of OFV causing citrus leprosis have been previously detected in the Mexican states of Chiapas, Querétaro, and Jalisco, we promote a pertinent discussion and thought-provoking questions regarding the epidemiology and putative evolution of OFV.

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Data availability

Nucleotide sequences of fragments corresponding to the N and L genes of OFV_Mex1 obtained in this study were deposited in the GenBank accessions MT497517and MT497518, respectively.

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Acknowledgments

The authors are grateful to Vincenzo Bertolini, El Colegio de la Frontera Sur, México, for the identification of the orchid plant.

Funding

This research was funded by Fundação de Apoio à Pesquisa do Estado de São Paulo- FAPESP, grants 2014/08458-9 and 2017/50222-0. CCJ and ADT have scholarships from FAPESP, respectively 2016/01960-6 and 2018/12252-8. In addition, the study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior- Brasil (CAPES) (001. PNPD20132154-33141010001P4-PNPD-IBSP).

Author information

Authors and Affiliations

  1. Campus Montecillo, Colegio de Potsgraduados, 56320, Montecillo, Edo. México, México

    Gabriel Otero-Colina

  2. Laboratório de Biologia Molecular Aplicada, Instituto Biológico, Av. Conselheiro Rodrigues Alves 1252, São Paulo, SP, 04014-902, Brazil

    Pedro Luis Ramos-González, Camila Chabi-Jesus, Juliana Freitas-Astúa & Aline D. Tassi

  3. PPG Microbiologia Agrícola, ESALQ/USP, CP 9, Piracicaba, SP, 13418-900, Brazil

    Camila Chabi-Jesus

  4. Embrapa Mandioca e Fruticultura, Cruz das Almas, BA, 44380-000, Brazil

    Juliana Freitas-Astúa

  5. Departamento de Fitopatologia e Nematologia, ESALQ/USP, CP 9, Piracicaba, SP, 13418-900, Brazil

    Aline D. Tassi & Elliot W. Kitajima

Authors
  1. Gabriel Otero-Colina
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  2. Pedro Luis Ramos-González
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  3. Camila Chabi-Jesus
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  4. Juliana Freitas-Astúa
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  5. Aline D. Tassi
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  6. Elliot W. Kitajima
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Contributions

PLRG and EWK conceptualized and wrote the original draft; GOC, PLRG, CCJ, ADT, and EWK worked on the formal analysis; PLRG, JFA, and EWK supervised the study; JFA and EWK were responsible for funding acquisition. PLRG, CCJ, ADT, and EWK worked on the investigation and methodology. All authors reviewed the final manuscript.

Corresponding authors

Correspondence to Pedro Luis Ramos-González or Elliot W. Kitajima.

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Otero-Colina, G., Ramos-González, P.L., Chabi-Jesus, C. et al. First detection of orchid fleck virus in orchids in Mexico. VirusDis. 32, 167–172 (2021). https://doi.org/10.1007/s13337-021-00676-5

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