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Sapovirus detection and quantification in fecal samples from allogeneic hematopoietic stem cell transplant recipients

VirusDisease volume 31, pages 374–377 (2020)Cite this article

Abstract

Sapovirus are important agents of acute gastroenteritis (AGE) and they are associated with outbreaks and sporadic cases worldwide. They infect people of all ages, but mainly children, the elderly and immunocompromised individuals are affected. The aim of this study was investigate sapovirus and to determine viral loads in fecal samples from patient undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). Fecal samples were submitted to extraction of the genetic material using a commercial kit, and RT-qPCR TaqMan was used for sapovirus screening and determination of viral loads, using a standard curve with serial dilutions of a recombinant plasmid. Positive samples were sequence by Sanger method. Sapovirus was detected in one patient, 5.3% (1/19). Viral excretion lasted for 16 days. Viral load varied from 1.73 × 106 to 8.97 × 106 GC/g. One of the positive samples was characterized as GI.1 genotype. This is the first study to determine sapovirus loads in samples from allo-HSCT and to identify GI.1 genotype in immunocompromised patients.

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References

  1. Ahmed SM, Hall AJ, Robinson AE, Verhoef L, Premkumar P, Parashar UD, Koopmans M, Lopman BA. Global prevalence of norovirus in cases of gastroenteritis: a systematic review and meta-analysis. Lancet. 2014;14:725–30.

    Article  Google Scholar 

  2. Costa BCL, Dábilla NAS, Almeida TN, Fiaccadori FS, de Souza TT, Cardoso DDP, Arantes AM, Souza M. Human bocavirus detection and quantification in fecal and serum specimens from recipients of allogeneic hematopoietic stem cell transplantation: a longitudinal study. J Med Virol. 2019; 1–7.

  3. Daniel-Wayman S, Fahle G, Palmore T, Green KY, Prevots DR. Norovirus, astrovirus, and sapovirus among immunocompromised patients at a tertiary care research hospital. Diagn Microbiol Infect Dis. 2018;92:143–6.

    Article  Google Scholar 

  4. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95–8.

    CAS  Google Scholar 

  5. Lemes LG, Corrêa TS, Fiaccadori FS, Cardoso Dd, Arantes Ade M, Souza KM, Souza M. Prospective study on Norovirus infections among allogeneic stem cell transplant recipients: prolonged viral excretion and RNA viral in blood. J Clin Virol. 2014;61(3):329–33.

    CAS  Article  Google Scholar 

  6. Oka T, Katayama K, Hansman GS, Kageyama T, Ogawa S, Wu FT, White PA, Takeda N. Detection of human sapovirus by real-time reverse transcription-polymerase chain reaction. J Med Virol. 2006;78(10):1347–53.

    CAS  Article  Google Scholar 

  7. Oka T, Lu Z, Phan T, Delwart EL, Saif LJ, Wang Q. Genetic characterization and classification of human and animal sapoviruses. PLoS ONE. 2016;11(5):1–14.

    Article  Google Scholar 

  8. Oka T, Wang Q, Katayama K, Saif L. Comprehensive review of human sapoviruses. Clin Microbiol Rev. 2015;28:32–533.

    CAS  Article  Google Scholar 

  9. Oliveira DMM, Souza M, Fiaccadori FS, Santos HCP, Cardoso DDP. Monitoring of calicivirus among day-care children: Evidence of asymptomatic viral excretion and first report of GI.7 norovirus and GI.3 sapovirus in Brazil. J Med Virol. 2013;86:1569–75.

    Article  Google Scholar 

  10. Pietsch C, Liebert UG. Intrahost viral evolution during chronic sapovirus infections. J Clin Virol. 2019;113:1–7.

    Article  Google Scholar 

  11. Roddie C, Paul JPV, Benjamin R, Gallimore CI, Xerry J, Gray JJ, Peggs KS, Morris EC, Thomson KJ, Ward KN. Allogeneic hematopoietic stem cell transplantation and norovirus gastroenteritis: a previously unrecognized cause of morbidity. Clin Infect Dis. 2009;49(7):1061–8.

    CAS  Article  Google Scholar 

  12. Roos-Weil D, Ambert-Balay K, Lanternier F. Impact of norovirus/sapovirus-related diarrhea in renal transplant recipients hospitalized for diarrhea. Transplantation. 2011;92:61–9.

    Article  Google Scholar 

  13. Santos HCP, Almeira TNV, Fiaccadori FS, Cardoso DDP, Arantes AM, da Silva HD, Nagib PRA, Souza M. Adenovirus infection among allogeneic stem cell transplant recipients. J Med Virol. 2017;89:298–303.

    Article  Google Scholar 

  14. Schuster JE, Johnston SH, Piva B, Dulek DE, Wikswo ME, McHenry R, Browne H, Gautam R, Bowen MD, Vijé J, Payne DC, Azimi P, Selvrangan R, Halasa N, Englund JA. Infectious causes of acute gastroenteritis in US children undergoing allogeneic hemtopoietic cell transplant: a longitudinal, multicnter study. J Pediatr Infect Dis Soc. 2019. https://doi.org/10.1093/jpids/piz063.

    Article  Google Scholar 

  15. Tomblyn M, Chiller T, Einsele H, Gress R, Sepkowitz K, Storek J, Wingard JR, Young JA, Boeckh MJ. Guidelines for preventing infectious complications among hematopoietic cell transplantation recipients: a global perspective. Biol Blood Marrow Transplant. 2009;15:1143–238.

    CAS  Article  Google Scholar 

  16. Yan H, Yagyu F, Okitsu S, Nishio O, Ushijima H. Detection of Norovirus [GI, GII], Sapovirus and astrovirus in fecal samples using reverse transcription single-round multiplex PCR. J Virol Methods. 2003;114:37–44.

    CAS  Article  Google Scholar 

Download references

Acknowledgments

We thank all the staff members from the bone marrow transplant unit of Hospital Araujo Jorge who collected the patients' samples. This study was supported by Grant of Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (MCTI/CNPq/Universal 2014). The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.

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Author notes

  1. Thairiny Neres Silva and Nathânia Dábilla have contributed equally to this work.

Affiliations

  1. Laboratory of Virology and Cell Culture, Instituto de Patologia Tropical E Saúde Pública, Universidade Federal de Goiás - UFG, Rua 235, s/n, sala 420, Setor Universitário, Goiânia, GO, 74605050, Brazil

    Thairiny Neres Silva, Nathânia Dábilla, Thaís Santos Corrêa & Menira Souza

  2. Hospital Araújo Jorge/Associação de Combate Ao Câncer Em Goiás, Goiânia, GO, Brazil

    Adriano de Moraes Arantes

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  1. Thairiny Neres Silva
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  2. Nathânia Dábilla
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  3. Thaís Santos Corrêa
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  4. Adriano de Moraes Arantes
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  5. Menira Souza
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Correspondence to Menira Souza.

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Neres Silva, T., Dábilla, N., Santos Corrêa, T. et al. Sapovirus detection and quantification in fecal samples from allogeneic hematopoietic stem cell transplant recipients. VirusDis. 31, 374–377 (2020). https://doi.org/10.1007/s13337-020-00583-1

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  • DOI: https://doi.org/10.1007/s13337-020-00583-1

Keywords

  • Sapovirus
  • Immunocompromised patients
  • GI.I sapovirus
  • Allo-HSCT
  • RT-qPCR