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Molecular evidence of human papillomaviruses in the retinoblastoma tumor

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Abstract

Retinoblastoma tumor (RB) is one of the most prevalent ocular cancers among children. RB may be caused by inherited mutations in RB1 gene as well as some environmental risk factors. Human papillomaviruses (HPV) are suspected as a risk factor of RB due to their pRb inactivating protein. This study evaluated the molecular prevalence of HPV among the RB tumor specimens in Iran. The RB tumor samples were tested for detection of HPV-L1 gene using a nested-PCR approach, and then followed by sequencing and phylogenetic analysis to reveal HPV types. Overall, there were 61 RB tumor samples; 54/61 (88.5%) had unilateral and 7/61 (11.5%) bilateral RB; 55/61 cases (90.2%) had sporadic non-familial RB tumor. HPV-DNA was detected in 6/61 (9.8%) of patients’ tumors; the HPV positive RB cases all had unilateral and unfamiliar sporadic RB tumor. HPV type 16 was the most prevalent type identified across the RB tumor samples (3/61, 4.9%). The rate of detected HPV among the RB specimens seems to be considerable. Further investigations are required to elucidate the exact association between HPV and progression to RB.

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Author information

Authors and Affiliations

  1. Department of Virology, Iran University of Medical Sciences, Tehran, Iran

    Davod Javanmard, Masood Moein, Maryam Esghaei, Seyed Hamidreza Monavari & Farah Bokharaei‐Salim

  2. Eye Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran

    Masood Naseripour

  3. HIV Laboratory of National Center, Deputy of Health, Iran University of Medical Sciences, Tehran, Iran

    Farah Bokharaei‐Salim

  4. Department of Pathology, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran

    Alireza Sadeghipour

Authors
  1. Davod Javanmard
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  2. Masood Moein
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Correspondence to Maryam Esghaei.

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Javanmard, D., Moein, M., Esghaei, M. et al. Molecular evidence of human papillomaviruses in the retinoblastoma tumor. VirusDis. 30, 360–366 (2019). https://doi.org/10.1007/s13337-019-00540-7

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  • DOI: https://doi.org/10.1007/s13337-019-00540-7

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