Abstract
In Egypt, recent outbreaks were reported in NDV-vaccinated flocks. The isolated strain was characterized as class II velogenic genotype VIId of Newcastle disease virus (NDV). In this study, three inactivated NDV vaccine formulations were prepared, the first one is LaSota (genotype II), the second one is genotype VIId and the third one is combined Lasota and genotype VIId at a proportion of 1:1. The challenge trials were conducted in SPF chicks to evaluate the efficacy of the prepared vaccines using 106 EID50/0.5 ml of the Egyptian genotype VIId strain of Newcastle disease virus (NDV-B7-RLQP-CH-EG-12). Our results revealed that all three prepared vaccine formulations conferred 100% protection in the vaccinated chicks. However, the combined vaccine induced the highest haemagglutination inhibition (HI) titers and neutralization indices with significant reduction in virus shedding compared to other vaccine formulations. Histopathology examination of different organs collected from vaccinated chicks post challenge indicated the protective efficacy in vaccinated groups compared to the positive control group where a score of severe lesions was shown. This study reports the efficacy of combined inactivated Lasota and genotype VIId vaccine in reducing virus shedding which is very important in controlling NDV infection in chicken.
Similar content being viewed by others
References
Alexander DJ. Newcastle disease. Developments in veterinary virology. Dordrecht: Kluwer Academic Publishers; 1988.
Alexander DJ, Senne DA. Newcastle disease, other avian paramyxoviruses, and pneumovirus infections. Dis Poult. 2008;19:75–115.
Bahnemann HG. Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine. Vaccine. 1990;8:299–303.
Bancroft JD, Layton C. The hematoxylins and eosin. Bancroft’s Theory and Practice of Histological Techniques. 2013. p. 173–86.
Dortmans JCFM, Peeters BPH, Koch G. Newcastle disease virus outbreaks: Vaccine mismatch or inadequate application? Vet Microbiol. 2012;160:17–22.
Hu Z, Hu S, Meng C, Wang X, Zhu J, Liu X. Generation of a genotype VII Newcastle disease virus vaccine candidate with high yield in embryonated chicken eggs. Avian Dis Digest. 2011;6:e7–8.
Hu Z, Hu J, Hu S, Liu X, Wang X, Zhu J, et al. Strong innate immune response and cell death in chicken splenocytes infected with genotype VIId Newcastle disease virus. Virol J. 2012;9:208.
Hussein HA, Emara MM, Rohaim MA. Molecular characterization of Newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt. Int J Virol. 2014;10:46–54.
Kapczynski DR, Afonso CL, Miller PJ. Immune responses of poultry to Newcastle disease virus. Dev Comp Immunol. 2013;41:447–53.
Kilany WH, Abdelwhab EM, Arafa AS, Selim A, Safwat M, Nawar AA, et al. Protective efficacy of H5 inactivated vaccines in meat turkey poults after challenge with Egyptian variant highly pathogenic avian influenza H5N1 virus. Vet Microbiol. 2011;150:28–34.
Lamb RA, Collins PL, Kolakofsky D, Melero JA, Nagai Y, Oldstone MBA, et al. Family paramyxoviridae. Virus taxonomy : the classification and nomenclature of viruses. The eighth report of the international committee on taxonomy of viruses. 2005. p. 655–68.
Miller PJ, King DJ, Afonso CL, Suarez DL. Antigenic differences among Newcastle disease virus strains of different genotypes used in vaccine formulation affect viral shedding after a virulent challenge. Vaccine. 2007;25:7238–46.
Miller PJ, Estevez C, Yu Q, Suarez DL, King DJ. Comparison of viral shedding following vaccination with inactivated and live Newcastle disease vaccines formulated with wild-type and recombinant viruses. Avian Dis. 2009;53:39–49. https://doi.org/10.1637/8407-071208-Reg.1.
Miller PJ, Decanini EL, Afonso CL. Newcastle disease: evolution of genotypes and the related diagnostic challenges. Infect Genet Evol. 2010;10:26–35.
Miller PJ, Afonso CL, El Attrache J, Dorsey KM, Courtney SC, Guo Z, et al. Effects of Newcastle disease virus vaccine antibodies on the shedding and transmission of challenge viruses. Dev Comp Immunol. 2013;41:505–13.
Palya V, Kiss I, Tatár-Kis T, Mató T, Felföldi B, Gardin Y. Advancement in vaccination against newcastle disease: recombinant HVT NDV provides high clinical protection and reduces challenge virus shedding with the absence of vaccine reactions. Avian Dis. 2012;56:282–7. https://doi.org/10.1637/9935-091511-Reg.1.
Panshin A, Shihmanter E, Weisman Y, Orvell C, Lipkind M. Antigenic heterogeneity amongst the field isolates of Newcastle disease virus (NDV) in relation to the vaccine strain. Part II: studies on viruses isolated from domestic birds in Israel. Comp Immunol Microbiol Infect Dis. 2002;25:173–85.
Pedersen JC, Senne DA, Woolcock PR, Kinde H, King DJ, Wise MG, et al. Phylogenetic relationships among virulent newcastle disease virus isolates from the 2002–2003 outbreak in California and other recent outbreaks in North America. J Clin Microbiol. 2004;42:2329–34.
Perozo F, Marcano R, Afonso CL. Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: efficacy of field vaccination. J Clin Microbiol. 2012;50:1204–8.
Radwan MM, Darwish SF, El-Sabagh IM, El-Sanousi AA, Shalaby MA. Isolation and molecular characterization of Newcastle disease virus genotypes II and VIId in Egypt between 2011 and 2012. Virus Genes. 2013;47:311–6.
Reed LJ, Muench H. A simple method of estimating fifty percent endpoints. Am J Hyg. 1938;27:493–7.
Roohani K, Tan SW, Yeap SK, Ideris A, Bejo MH, Omar AR. Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV. J Vet Sci. 2015;16:447–57.
Roy P, Venugopalan AT, Manvell R. Characterization of Newcastle disease viruses isolated from chickens and ducks in Tamilnadu, India. Vet Res Commun. 2000;24:135–42.
Rue CA, Susta L, Cornax I, Brown CC, Kapczynski DR, Suarez DL, et al. Virulent Newcastle disease virus elicits a strong innate immune response in chickens. J Gen Virol. 2011;92:931–9.
Saif YM. Immunosuppression induced by infectious bursal disease virus. Vet Immunol Immunopathol. 1991;30:45–50.
Sedeik ME, Elbestawy AR, El-Shall NA, Abd El-Hack ME, Saadeldin IM, Swelum AA. Comparative efficacy of commercial inactivated Newcastle disease virus vaccines against Newcastle disease virus genotype VII in broiler chickens. Poult Sci. 2019;98:2000–7.
Susta L, Miller PJ, Afonso CL, Brown CC. Clinicopathological characterization in poultry of three strains of Newcastle disease virus isolated from recent outbreaks. Vet Pathol. 2011;48:349–60.
Thayer SG, Beard Charles W. Serological procedures. In: Swayne D, Glisson J, Jackwood M, Pearson J, Reed W, editors. A laboratory manual for the isolation and identification and characterization of avian pathogens. 5th ed. Washington: American Association of Avian Pathologists; 2008. p. 222–5.
Van Boven M, Bouma A, Fabri THF, Katsma E, Hartog L, Koch G. Herd immunity to Newcastle disease virus in poultry by vaccination. Avian Pathol. 2008;37:1–5.
Wakamatsu N, King DJ, Seal BS, Peeters BPH, Brown CC. The effect on pathogenesis of Newcastle disease virus LaSota strain from a mutation of the fusion cleavage site to a virulent sequence. Avian Dis. 2006;50:483–8.
Wang Y, Duan Z, Hu S, Kai Y, Wang X, Song Q, et al. Lack of detection of host associated differences in Newcastle disease viruses of genotype VIId isolated from chickens and geese. Virol J. 2012;9:197.
Wise MG, Suarez DL, Seal BS, Pedersen JC, Senne DA, King DJ, et al. Development of a real-time reverse-transcription pcr for detection of Newcastle disease virus RNA in clinical samples. J Clin Microbiol. 2004;42:329–38.
World Organization for Animal Health (OIE). Newcastle disease (infection with Newcastle disease virus). Manual of diagnostic tests and vaccines for terrestrial animals : (mammals, birds and bees), vol. 1. Paris: OIE; 2012. p. 555–74.
Zenglei H, Liu X. Preview NDV induced immune-pathology in chickens. Br J Virol. 2015;2:25.
Acknowledgements
We are grateful for all Staff members of Newcastle Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Cairo, Egypt for their great help during the study.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
The use of animals and protocols were approved by the Animal Care and Use Committee of Veterinary Serum and Vaccine Research Institute, Egypt.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Mahmoud, N.K., El-Deeb, A.H., Emara, M.M. et al. Genotypes II and VIId-based inactivated Newcastle disease vaccine reduces virus shedding. VirusDis. 30, 453–461 (2019). https://doi.org/10.1007/s13337-019-00537-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13337-019-00537-2