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In silico analyses of molecular interactions between groundnut bud necrosis virus and its vector, Thrips palmi

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Abstract

Groundnut bud necrosis virus (GBNV) is an economically important tospovirus transmitted by Thrips palmi (Thysanoptera: Thripidae). The current understanding of thrips-tospovirus interactions is largely based on the tomato spotted wilt virus-Frankliniella occidentalis relationship. Only limited information is available for the GBNV-T. palmi system. In the present study, available genome data of T. palmi and GBNV were used to predict the protein partners that may play a crucial role in the internalization of GBNV virions into thrips cells. Computational analyses showed that the GBNV precursor glycoprotein bears a signal peptide of 24 amino acids and a secondary cleavage site at position 434–435 separates the amino-terminal mature glycoprotein (GN) from the carboxyl-terminal glycoprotein (GC). Potential interactions of GBNV glycoproteins were predicted with T. palmi enolase, cathepsin, C-type lectin, clathrin and vacuolar ATP synthase subunit E. The in silico analyses suggested that C-type lectin is the primary cellular receptor to interact with GBNV-GN. After receptor binding, virus particles probably enter vector cells by clathrin-mediated endocytosis. This is the first in silico evidence of GBNV-T. palmi protein interaction.

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Acknowledgments

The authors are thankful to Dr. Ralf. G. Dietzgen (QAAFI, the University of Queensland) for thorough read and editing of the final manuscript. We thank Dr. Dinesh Gupta (ICGEB, New Delhi) and Dr. Abhishek Mandal (IARI, New Delhi) for suggestions and discussions during the analyses. Two anonymous reviewers are thanked for critically reading the manuscript and suggesting substantial improvements. The research was supported by the research grants of IARI, and DBT (BT/PR26136/AGIII/103/1005/2018).

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  1. Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, 411007, India

    Shounak S. Jagdale

  2. Insect Vector Laboratory, Advanced Centre for Plant Virology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Amalendu Ghosh

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Jagdale, S.S., Ghosh, A. In silico analyses of molecular interactions between groundnut bud necrosis virus and its vector, Thrips palmi. VirusDis. 30, 245–251 (2019). https://doi.org/10.1007/s13337-019-00521-w

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