Abstract
The aim of the present study was to characterize the virus from the lesions and histopathology of organs associated with mortality in Kuroiler (dual purpose variety of poultry developed and marketed by Keggfarms Pvt. Ltd, India) birds suspected of Marek’s disease. Among 1047 birds from two farms of different location with 5.5 and 34% mortality, two types of lesion were observed in post mortem examination; tumors in vital organs—liver, spleen, kidney, lung and ovaries and generalized small nodular tumour in the abdominal cavity. Molecular characterization based on detection of ICP4 gene showed the presence of Marek’s disease virus (MDV) from tissues and cell culture adapted isolates in Madin Darby Canine Kidney cell lines. Histopathological examination revealed multinucleated immature lymphoid cells infiltration in the organs. Phylogenetic analysis of the isolates based on meq gene showed the isolates belongs to cluster I genotype of MDV. This is for the first time the MDV virus is characterized from an outbreak in the poultry flock in farmer’s field affecting production in Meghalaya state of North east India.
Similar content being viewed by others
References
Arulmozhi A, Saravanan S, Mohan B, Balasubramaniam GA. Marek’s disease in vaccinated poultry flocks in and around Namakkal region of Tamil Nadu. Indian J Vet Pathol. 2011;35:45–7.
Baigent SJ, Nair VK, Galludec HL. Real-time PCR for differential quantification of CVI988 vaccine virus and virulent strains of Marek’s disease virus. J Virol Methods. 2016;233:23–36.
Davidson I. The knowledge that human tumor virology can gain from studies on avian tumor viruses. Adv Tumor Virol. 2009;1:9–19.
Davidson I. Differential diagnosis of avian oncogenic viruses. 2001. http://ww.poultrymed.com. Accessed 6 Oct 2009.
Davidson I. Veiled and unveiled aspects of infection with oncogenic viruses. Keynote lecture article. In: Proceedings of the 14th world veterinary poultry congress, Istanbul, Turkey. 2005.
Davison F, Nair V. Use of Marek’s disease vaccines: could they be driving the virus to increasing virulence? Expert Rev Vaccines. 2005;4:77–88.
Gimeno IM. Marek’s disease vaccines: a solution for today but a worry for tomorrow? Vaccine. 2008;26(3):31–41.
Gopal S, Manoharan P, Kathaperumal K, Chidambaram B, Divyaa KC. Differential detection of avian oncogenic viruses in poultry layer farms and turkeys by use of multiplex PCR. J Clin Microbiol. 2012;50(8):2668–73.
Gupta M, Deka D, Ramneek. Sequence analysis of Meq oncogene among Indian isolates of Marek’s disease herpesvirus. Meta Gene. 2016;9:230–6.
Kalyani HI, Tajpara MM, Jhala MK, Bhanderi BB, Nayak JB, Purohit JH. Characterization of the ICP4 gene in pathogenic Marek’s disease virus of poultry in Gujarat, India, using PCR and sequencing. Veterinarski Arhiv. 2010;80(5):683–92.
Lee SI, Takagi M, Ohashi K, Sugimoto C, Onuma M. Difference in the meq gene between oncogenic and attenuated strains Marek’s disease virus serotype 1. J Vet Med Sci. 2000;62:287–92.
Mohamed MA, Tolba Y, El-Motelib A, Ibrahim AA, El-Deen S, Moustafa E. Contamination rate of Avian Leukosis viruses among commercial Marek’s Disease vaccines in Assiut, Egypt market using Reverse Transcriptase-Polymerase Chain Reaction. Vet World. 2010;3(1):08–12.
Nair V. Evolution of Marek’s disease: a paradigm for incessant race between the pathogen and the host. Vet J. 2005;170:175–83.
Nair V. Latency and tumorigenesis in Marek’s disease. Avian Dis. 2013;57:360–5.
Payne LN, Venugopal K. Neoplastic diseases: Marek’s disease, avian leukosis and reticuloendotheliosis. Rev Sci Tech Off Int Epizoot. 2000;19(2):544–64.
Peng H, Qin L, Bi Y, Wang P, Zou G, Li J, Yang Y, Zhong X, Wei P. Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification. Virol J. 2015;12:195–212.
Renz KG, Cooke J, Clarke N, Cheetham BF, Hussain Z, Fakhrul Islam AF, Tannock GA, Walkden-Brown SW. Pathotyping of Australian isolates of Marek’s disease virus and association of pathogenicity with Meq gene polymorphism. Avian Pathol. 2012;41:161–76.
Salter DW, Crittenden LB. Artificial insertion of a dominant gene for resistance to avian leukosis virus into the germ line of the chicken. Theor Appl Genet. 1989;77:457–61.
Shamblin CE, Greene N, Arumugaswami V, Dienglewicz RL, Parcells MS. Comparative analysis of Marek’s disease virus (MDV) glycoprotein-, lytic antigen pp38- and transformation antigen Meq-encoding genes: association of meq mutations with MDVs of high virulence. Vet Microbiol. 2004;102:147–67.
Silva RF, Fadly AM, Taylor SP. Development of a polymerase chain reaction to differentiate avian leukosis virus (ALV) subgroups: detection of an ALV contaminant in commercial Marek’s disease vaccines. Avian Dis. 2007;51:663–7.
Venugopal K, Howes K, Flannery DMJ, Payne LN. Isolation of acutely transforming subgroup J avian leukosis viruses that induce erythroblastosis and myelocytomatosis. Avian Pathol. 2000;29:327–32.
Wang J, Meers J, Spradbrow PB, Robinson WF. Evaluation of immune effects of fowlpox vaccine strains and field isolates. Vet Microbiol. 2006;116:106–19.
Wilhelmsen KC, Eggleton K, Temin HM. Nucleic acid sequences of the oncogene v-rel in reticuloendotheliosis virus strain T and its cellular homolog, the proto-oncogene c-rel. J Virol. 1984;52:172–82.
Witter RL, Fadly AM. Reticuloendotheliosis. In: Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR, Swayne DE, editors. Diseases of poultry. Ames: Iowa State University Press; 2003. p. 517–35.
Witter RL, Fadly AM. Reticuloendotheliosis. In: Saif YM, et al., editors. Diseases of poultry. 11th ed. Ames: Iowa State University Press; 2003. p. 517–35.
Yu ZH, Teng M, Luo J, Wang XW, Ding K, Yu LL, Su JW, Chi JQ, Zhao P, Hu B, Zhang GP, Liu JX. Molecular characteristics and evolutionary analysis of field Marek’s disease virus prevalent in vaccinated chicken flocks in recent years in China. Virus Genes. 2013;47(2):282–91.
Acknowledgements
The authors would like to acknowledge Professor Vandana Raphael, Dean Cum HOD, Deptt of Pathology, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Shillong, Meghalaya and her team for help in preparation of histopathological slides and photographs. The research work is institute project (code IXX11689) of Indian Council of Agriculture Research - Research Complex for North Eastern Hill Region, Umiam, Meghalaya.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Puro, Ku., Bhattacharjee, U., Baruah, S. et al. Characterization of Marek’s disease virus and phylogenetic analyses of meq gene from an outbreak in poultry in Meghalaya of Northeast India. VirusDis. 29, 167–172 (2018). https://doi.org/10.1007/s13337-018-0448-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13337-018-0448-2