Advertisement

VirusDisease

, Volume 29, Issue 2, pp 203–206 | Cite as

Molecular evidence for the occurrence of TYLCV on Mentha longifolia in Jeddah, Saudi Arabia

  • Sayed Sartaj Sohrab
  • Ihsanullah Daur
Short Communication

Abstract

Begomoviruses are whiteflies transmitted virus causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. Mentha is an important crop being grown here in Saudi Arabia for various purposes. The begomovirus associated disease was observed on Mentha crops during field survey which were growing near to tomato field. There is no published report available about the association of begomovirus on Mentha from this region. So, this work was conducted to identify the causative agent associated with yellow vein mosaic disease. Naturally infected samples were collected from various locations and causative agent was identified by PCR using begomovirus specific primers and further cloned and sequenced bidirectionally. The full genome had total 2785 nucleotides while betasatellite molecule had 1365 nucleotides. Based on full-genome sequence analysis, the identity matrix and phylogenetic analysis showed the highest identity (99.6%) with Tomato yellow leaf curl virus (TYLCV) reported from tomato in the Kingdom of Saudi Arabia. The identified begomovirus was observed as isolate of TYLCV.

Keywords

M. longifolia Begomovirus TYLCV Genetic diversity Phylogeny 

Notes

Acknowledgements

Author would like to thank and gratefully acknowledge the research facility provided by Special Infectious Agents Unit, King Fahd Medical Research Center (KFMRC), King Abdulaziz University, Jeddah, Saudi Arabia.

References

  1. 1.
    Ahmed AM, Ozbak HA, Hemeg HA. Effect of essential oil of traditional two Saudi mint types and its possible role in cardiovascular and throat health. Int J Clin Exp Med. 2015;5:8060–8.Google Scholar
  2. 2.
    Brown JK, Zerbini FM, Castillo JN, Moriones E, Sobrinho RR, Silva JCF, Olive EF, Briddon RW, Zepeda CHN, Idris A, Malathi VG, Martin DP, Bustamante RR, Ueda S, Varsani A. Revision of begomovirus taxonomy based on pair wise sequence comparisons. Arch Virol. 2015;60:1593–619.CrossRefGoogle Scholar
  3. 3.
    Kenyon L, Tsai WS, Shih SL, Lee LM. Emergence and diversity of begomoviruses infecting Solanaceous crops in East and Southeast Asia. Virus Res. 2014;186:104–13.CrossRefPubMedGoogle Scholar
  4. 4.
    Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870–4.  https://doi.org/10.1093/molbev/msw054.CrossRefPubMedGoogle Scholar
  5. 5.
    Muhire BM, Varsani A, Martin DP. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE. 2014;9(9):e108277.  https://doi.org/10.1371/journal.pone.0108277.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Saeed ST, Samad A. Emerging threats of begomoviruses to the cultivation of medicinal and aromatic crops and their management strategies. Virus Dis. 2017;28(1):1–17.CrossRefGoogle Scholar
  7. 7.
    Saeed ST, Khan A, Kumar B. Ajay kumar PV, Samad A. First report of Chili leaf curl India virus infecting Mentha spicate (Neera) in India. Plant Dis. 2014;98:164.CrossRefGoogle Scholar
  8. 8.
    Saeed ST, Kumar B, Shasany AK, Samad A. Molecular identification of Chili leaf curl India virus along with betasatellite molecule causing leaf curl disease of menthol mint (Mentha arvensis var. Kosi) in India. J Gen Plant Pathol. 2017;83:333–6.CrossRefGoogle Scholar
  9. 9.
    Sohrab SS. Tomato leaf curl Sudan virus causing leaf curl disease on a new host Amaranthus cruentus L. Plantomics J. 2016;10:20–7.Google Scholar
  10. 10.
    Sohrab SS. The role of Corchorus in spreading of Tomato yellow leaf curl virus on tomato in Jeddah, Saudi Arabia. Virus Dis. 2016;27:19–26.CrossRefGoogle Scholar
  11. 11.
    Sohrab SS, Yasir M, El-Kafrawy SA, Abbas AT, Mousa MAA, Bakhashwain AA. Association of Tomato leaf curl Sudan virus with leaf curl disease of tomato in Jeddah. Saudi Arabia. Virus Dis. 2016;27:145–53.Google Scholar
  12. 12.
    Sohrab SS, Yasir M, El-Kafrawy SA, Al-Zahrani HSM, Mousa MAA, Bakhashwain AA. Phylogenetic relationships, recombination analysis and genetic variability of Tomato yellow leaf curl virus infecting tomato in Jeddah, Saudi Arabia. Plantomics J. 2016;9(1):90–8.Google Scholar
  13. 13.
    Sohrab SS, Yasir M, El-Kafrawy SA. Association of Tomato leaf curl Sudan virus with leaf curl disease of Squash in Jeddah, Saudi Arabia. Agrica. 2016;28–34:145–53.Google Scholar
  14. 14.
    Sohrab SS, Yasir M, El-Kafrawy SA. Begomovirus infection on Cucumber in Saudi Arabia. Plantomics J. 2016;10:7–14.Google Scholar
  15. 15.
    Tzanetakis IE, Postman JD, Samad A, Martin RR. Mint viruses: beauty, stealth and disease. Plant Dis. 2010;94:4–12.CrossRefGoogle Scholar
  16. 16.
    Varma A, Mandal B, Singh MK. Global emergence and spread of whitefly (Bemisia tabaci) transmitted Geminiviruses. In: Thompson WMO, editor. The whitefly, Bemisia tabaci (Homoptera: Aleyrodidae) interaction with geminivirus-infected host plants. Berlin: Springer; 2011. p. 205–92.CrossRefGoogle Scholar
  17. 17.
    Zerbini FM, Briddon RW, Idris A, Martin DP, Moriones E, Navas-Castillo J, Rivera-Bustamante R, Roumagnac P, Varsani A. ICTV report consortium. ICTV virus taxonomy profile: geminiviridae. J Gen Virol. 2017;98(2):131–3.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Indian Virological Society 2018

Authors and Affiliations

  1. 1.Special Infectious Agents Unit, King Fahd Medical Research Center (KFMRC)King Abdulaziz UniversityJeddahSaudi Arabia
  2. 2.Department of Arid Land Agriculture, Faculty of Meteorology Environment and Arid Land AgricultureKing Abdulaziz UniversityJeddahSaudi Arabia

Personalised recommendations