Advertisement

VirusDisease

, Volume 29, Issue 2, pp 199–202 | Cite as

Comparison of a rapid immuno-chromatography assay with a standard ELISA for the detection of IgM and IgG antibodies against dengue viruses

  • Kalamathy Murugananthan
  • Pethirupillai A. D. Coonghe
  • Natkunam Ketheesan
  • Faseeha Noordeen
Short Communication
  • 75 Downloads

Abstract

A total of 765 blood samples collected from dengue suspected patients admitted to a Teaching Hospital in Sri Lanka were used to compare a rapid ICT assay with a standard ELISA for the detection of anti-dengue virus (DENV) IgM and IgG. Detection accuracy indices including sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), Chi square and Cohen’s kappa values were determined for the ICT assay using the ELISA as a comparator for the detection of anti-DENV IgM and IgG. The rapid ICT assay showed a sensitivity of 64%, specificity of 75%, NPV of 68% and PPV of 72% for anti-DENV IgM detection. However, all the accuracy indices were relatively higher for the anti-DENV IgG detection by the ICT assay than those for anti-DENV IgM detection. Despite the low sensitivity for anti-DENV IgM detection by the ICT assay, considering the limitations in using ELISAs in resource limited regions, rapid ICT assays would be useful for the detection of more recent DENV infections. As many patients present after fever days 5 in the study area, anti-DENV IgM/IgG would be the suitable marker to be detected by rapid ICT assays in such areas.

Keywords

Dengue Rapid immuno-chromatography assays ELISA Anti-DENV IgM/IgG 

Notes

Acknowledgements

We thank the financial support provided from the Higher Education for the 21st Century (HETC) Grant (HETC JEN/O-MED/N7) from the Ministry of Higher Education, Sri Lanka and Australian High Commission’s Direct Aid Programme for ELISA kits.

References

  1. 1.
    Blacksell SD. Commercial dengue rapid diagnostic tests for point-of-care application: recent evaluations and future needs? J Biomed Biotechnol. 2012.  https://doi.org/10.1155/2012/151967.Google Scholar
  2. 2.
    Blacksell SD, Newton PN, Bell D, Kelley J, Mammen MP Jr, Vaughn DW, Wuthiekanun V, Sungkakum A, Nisalak A, Day NPJ. The comparative accuracy of 8 commercial rapid immuno-chromatographicassays for the diagnosis of acute dengue virus infection. Clin Infect Dis. 2006;42:1127–34.CrossRefPubMedGoogle Scholar
  3. 3.
    Blacksell SD, Jarman RG, Bailey MS, Tanganuchitcharnchai A, Jenjaroen K, Gibbons RV, Paris DH, Premaratna R, de Silva HJ, Lalloo DG, Day NP. Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: the need for combining NS1 antigen and IgM/IgG antibody detection to achieve acceptable levels of accuracy. Clin Vaccine Immunol. 2011;18:2095–101.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Fry SR, Meyer M, Semple MG, Simmons CP, Sekaran SD, Huang JX, McElnea C, Huang CY, Valks A, Young PR, Cooper MA. The diagnostic sensitivity of dengue rapid test assays is significantly enhanced using a combined antigen and antibody testing approach. PLoS Negl Trop Dis. 2011;21(5):e1199.CrossRefGoogle Scholar
  5. 5.
    Moorthy M, Chandy S, Selvaraj K, Abraham AM. Evaluation of a rapid immuno-chromatographic device for the detection of IgM and IgG antibodies to dengue viruses in a tertiary care hospital in south India. Indian J Med Microbiol. 2009;27:254–6.CrossRefPubMedGoogle Scholar
  6. 6.
    Nga TT, Thai KT, Phuong HL, Giao PT, Hung LQ, Binh TQ, Mai VT, Van NN, de Vries PJ. Evaluation of two rapid immuno-chromatographic assays for the diagnosis of dengue among Vietnamese febrile patients. Clin Vaccine Immunol. 2007;14:799–801.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Senaratne TN, Noordeen F. Diagnosis of dengue in Sri Lanka: improvements to the existing state of the art in the island. Trans R Soc Trop Med Hyg. 2014;108:685–91.CrossRefPubMedGoogle Scholar
  8. 8.
    Senaratne T, Noordeen F, Dissanayake N, Kumara K. Comparison of a rapid immuno-chromatography assay with an enzyme linked immunosorbent assay (ELISA) for anti-dengue virus IgM detection. Sri Lankan J Infect Dis. 2014;4:77–82.CrossRefGoogle Scholar
  9. 9.
    Subedi D, Taylor-Robinson AW. Development of a dengue vaccine in humans: so near, yet so far.SOJ. Vaccine Res. 2015;1:7.Google Scholar
  10. 10.
    Vickers IE, Harvey KM, Brown MG, Nelson K, DuCasse MB, Lindo JF. The performance of the SD BIOLINE Dengue DUO® rapid immuno-chromatographic test kit for the detection of NS1 antigen, IgM and IgG antibodies during a dengue type 1 epidemic in Jamaica. J Biomed Sci. 2015;22:55.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Indian Virological Society 2018

Authors and Affiliations

  • Kalamathy Murugananthan
    • 1
    • 5
  • Pethirupillai A. D. Coonghe
    • 2
  • Natkunam Ketheesan
    • 3
    • 4
  • Faseeha Noordeen
    • 5
  1. 1.Department of Microbiology, Faculty of MedicineUniversity of JaffnaJaffnaSri Lanka
  2. 2.Community and Family Medicine, Faculty of MedicineUniversity of JaffnaJaffnaSri Lanka
  3. 3.College of Public Health, Medical and Veterinary SciencesJames Cook UniversityTownsvilleAustralia
  4. 4.University of New EnglandArmidaleAustralia
  5. 5.Department of Microbiology, Faculty of MedicineUniversity of PeradeniyaPeradeniyaSri Lanka

Personalised recommendations