Abstract
Canine parvovirus (CPV) can cause severe disease in animals, especially in dogs, and continuously generates new variants. In this study, the complete VP2 genes of 59 CPV isolates from clinical diseased dogs between 2015 and 2016 in 4 Chinese provinces were sequenced and analyzed. The results showed that new CPV-2a was still the prominent CPV genotype, followed by CPV-2c and new CPV-2b. CPV-2c with Ala5Gly and Gln370Arg mutations was first detected in Henan, Guangxi and Jiangsu provinces in China. A phylogenetic tree based on VP2 sequences showed that all isolates in this study formed a close lineage which separated from foreign isolates. The above results indicated that point mutations in VP2 were constantly occurring along with the widespread of CPV in China.
References
Battilani M, Ciulli S, Tisato E, Prosperi S. Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy. Virus Res. 2002;83:149–57. https://doi.org/10.1016/S0168-1702(01)00431-2.
Decaro N, Cirone F, Desario C, Elia G, Lorusso E, Colaianni ML, et al. Severe parvovirus in a 12-year-old dog that had been repeatedly vaccinated. Vet Rec. 2009;164:593–5.
Decaro N, Desario C, Parisi A, Martella V, Lorusso A, Miccolupo A, et al. Genetic analysis of canine parvovirus type 2c. Virology. 2009;385:5–10. https://doi.org/10.1016/j.virol.2008.12.016.
Geng Y, Guo D, Li C, Wang E, Wei S, Wang Z, et al. Co-circulation of the rare CPV-2c with unique Gln370Arg substitution, new CPV-2b with unique Thr440Ala substitution, and new CPV-2a with high prevalence and variation in Heilongjiang Province, Northeast China. PLoS ONE. 2015;10:e0137288. https://doi.org/10.1371/journal.pone.0137288.
Guo L, Yang SL, Chen SJ, Zhang Z, Wang C, Hou R, et al. Identification of canine parvovirus with the Q370R point mutation in the VP2 gene from a giant panda (Ailuropoda melanoleuca). Virol J. 2013;10:163. https://doi.org/10.1186/1743-422X-10-163.
Hueffer K, Parker JSL, Weichert WS, Geisel RE, Sgro JY, Parrish CR. The natural host range shift and subsequent evolution of canine parvovirus resulted from virus-specific binding to the canine transferrin receptor. J Virol. 2003;77:1718–26. https://doi.org/10.1128/JVI.77.3.1718-1726.2003.
Lin CN, Chien CH, Chiou MT, Chueh LL, Hung MY, Hsu HS. Genetic characterization of type 2a canine parvoviruses from Taiwan reveals the emergence of an Ile324 mutation in VP2. Virol J. 2014;11:39. https://doi.org/10.1186/1743-422X-11-39.
Martella V, Decaro N, Elia G, Buonavoglia C. Surveillance activity for canine parvovirus in Italy. J Vet Med B Infect Dis Vet Public Health. 2005;52:312–5. https://doi.org/10.1111/j.1439-0450.2005.00875.x.
Mukhopadhyay HK, Matta SL, Amsaveni S, Antony PX, Thanislass J, Pillai RM. Phylogenetic analysis of canine parvovirus partial VP2 gene in India. Virus Genes. 2014;48:89–95. https://doi.org/10.1007/s11262-013-1000-5.
Parrish CR, O’Connell PH, Evermann JF, Carmichael LE. Natural variation of canine parvovirus. Science. 1985;230:1046–8.
Parrish CR, Have P, Foreyt WJ, Evermann JF, Senda M, Carmichael LE. The global spread and replacement of canine parvovirus strains. J Gen Virol. 1988;69:1111–6. https://doi.org/10.1099/0022-1317-69-5-1111.
Perez R, Francia L, Romero V, Maya L, Lopez I, Hernandez M. First detection of canine parvovirus type 2c in South America. Vet Microbiol. 2007;124:147–52. https://doi.org/10.1016/j.vetmic.2007.04.028.
Sobrino R, Arnal MC, Luco DF, Gortazar C. Prevalence of antibodies against canine distemper virus and canine parvovirus among foxes and wolves from Spain. Vet Microbiol. 2008;126:251–6. https://doi.org/10.1016/j.vetmic.2007.06.014.
Tsao J, Chapman MS, Agbandje M, Keller W, Smith K, Wu H, et al. The three-dimensional structure of canine parvovirus and its functional implications. Science. 1991;251:1456–64.
Wang J, Lin P, Zhao H, Cheng Y, Jiang Z, Zhu H, et al. Continuing evolution of canine parvovirus in China: isolation of novel variants with an Ala5Gly mutation in the VP2 protein. Infect Genet Evol. 2016;38:73–8. https://doi.org/10.1016/j.meegid.2015.12.009.
Xu J, Guo HC, Wei YQ, Shu L, Wang J, Li JS, et al. Phylogenetic analysis of canine parvovirus isolates from Sichuan and Gansu provinces of China in 2011. Transbound Emerg Dis. 2015;62:91–5. https://doi.org/10.1111/tbed.12078.
Yi L, Tong M, Cheng Y, Song W, Cheng S. Phylogenetic analysis of canine parvovirus VP2 gene in China. Transbound Emerg Dis. 2016;63:e262–9. https://doi.org/10.1111/tbed.12268.
Zhang RZ, Yang ST, Feng H, Gui CS, Xia XZ. The first detection of canine parvovirus type 2c in China. J Pathog Biol. 2010;5:246–75.
Zhao Y, Lin Y, Zeng X, Lu C, Hou J. Genotyping and pathobiologic characterization of canine parvovirus circulating in Nanjing, China. Virol J. 2013;10:272. https://doi.org/10.1186/1743-422X-10-272.
Zhou P, Zeng WJ, Zhang X, Li SJ. The genetic evolution of canine parvovirus—a new perspective. PLoS ONE. 2017. https://doi.org/10.1371/journal.pone.0175035.
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This study was supported by Grant from Luoyang Heluo Talent Plan (Dr. Kegong Tian).
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Wu, H., Li, X., Wang, L. et al. Molecular epidemiological survey of canine parvovirus in domestic dogs in four provinces, China. VirusDis. 29, 113–117 (2018). https://doi.org/10.1007/s13337-018-0427-7
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DOI: https://doi.org/10.1007/s13337-018-0427-7