Advertisement

VirusDisease

, Volume 27, Issue 1, pp 91–97 | Cite as

Seroprevelance and molecular detection of peste des petits ruminants in goats of Assam

  • Maitrayee DeviEmail author
  • Sutopa Das
  • Krishna Sharma
  • Rupam Dutta
Original Article

Abstract

The present study was undertaken to detect the presence of PPRV in the goats of Assam. Competitive ELISA and Sandwich ELISA are used to detect the PPR viral antibody and antigen respectively. In addition, the study also involved the assessment of specific gene targets for detection of PPRV by RT-PCR from the clinical samples. A total of 579 sera samples (68.65 % in outbreak samples and 5.29 % in random samples) collected from different parts of Assam were tested by c-ELISA, indicated overall prevalence of 27.28 in goats. The percentage prevalence of PPRV antibodies in sera samples from goats collected at the time of outbreaks were 79.26, 85.41, 58.82, 6, 29.41 and 36.36 % in Kamrup, Nalbari, Mongoldoi, Jorhat, Darrang and Barpeta respectively. However, high percent prevalence (20.83 %) was observed in district Dhubri in random samples. Among the suspected samples, high percent prevalence (85.41 %) was observed in Nalbari. The competition percentage values (ranges from 35 to 45) obtained in competitive ELISA from tested goat samples found three categories, viz. positive, doubtful and negative. Most of the serum samples (n = 158) with competition percentage less than or equal to 35 % are considered positive for the presence of PPRV antibodies, (n = 9) greater than 35 % and less than or equal to 45 % are considered doubtful and retested, and (n = 423) greater than 45 % are considered negative. The overall sensitivity, specificity, apparent prevalence and true prevalence rate was found to be 68.65, 94.70, 27.28 and 34.69 % respectively. True prevalence rate was calculated based on the sensitivity and specificity of the c-ELISA employed in the study, which has a relative specificity of 94.70 % and sensitivity of 68.65 %.

Keywords

PPR Sandwich ELISA cELISA RT-PCR 

Notes

Acknowledgments

The authors are thankful to the Department of Biotechnology, Government of India, for providing grant to carry out this research work. The authors are also grateful to Dr. P. Borah, Coordinator, State Biotech Hub, College of Veterinary Science, Assam Agricultural University for extending suggestion and encouragement time to time.

References

  1. 1.
    Abraham G, Sintayehu A, Libeau G, Albina E, Roger F, Laekemariam Y, Abayneh D, Awoke KM. Antibody sero-prevalences against peste des petits ruminants (PPR) virus in camels, cattle, goats and sheep in Ethiopia. Prev Vet Med. 2005;70:51–7.CrossRefPubMedGoogle Scholar
  2. 2.
    Abubakar M, Rajput ZI, Arshed MJ, Sarwar G, Ali Q. Evidence of peste des petitsruminants virus (PPRV) infection in Sindh Ibex (Capra aegagrusblythi) in Pakistan as confirmed by detection of antigen and antibody. Trop Anim Health Prod. 2011;43(4):745–7.CrossRefPubMedGoogle Scholar
  3. 3.
    Abu-Elzein EME, Hassanien MM, Al-Afaleq AI, Abd-Elhadi MA, Housawi FMI. Isolation of peste des petits ruminants from goats in Saudi Arabia. Vet Rec. 1990;127(12):309–10.PubMedGoogle Scholar
  4. 4.
    Abu-Elzein EME, Housawi FMT, Bashareek Y, Gameel AA, Al-Afaleq AI, Anderson EC. Severe PPR infection in Gazelles kept under semi-free range conditions in Saudi Arabia. J Vet Microbiol B. 2004;51(2):68–71.Google Scholar
  5. 5.
    Anonymous. All India Co-ordinated Research Project on Animal Disease Monitoring and Surveillance (AICRP on ADMAS). Annual report 2010–2011. Guwahati: AICRP on ADMAS Collaborating Unit; 2011. p. 77–8.Google Scholar
  6. 6.
    Balamurugan V, Sen A, Venkatesan G, et al. Isolation and Identification of virulent peste des petits ruminants viruses from PPR outbreaks in India. Trop Anim Health Prod. 2010;42:1043–6.CrossRefPubMedGoogle Scholar
  7. 7.
    Balamurugan V, Saravanan P, Sen A, Rajak KK, Venkatesan G, Krishnamoorthy P, Bhanuprakash V, Singh RK. Prevalence of peste des petits ruminants among sheep and goats in India. J Vet Sci. 2012;13(3):279–85.PubMedCentralCrossRefPubMedGoogle Scholar
  8. 8.
    Balamurugan V, Krishnamoorthy P, Raju DS, Rajak KK, Bhanuprakash V, Pandey AB, Gajendragad MR, Prabhudas K, Rahman H. Prevalence of peste-des-petits-ruminant virus antibodies in cattle, buffaloes, sheep and goats in India. Virusdisease. 2014;25(1):85–90.PubMedCentralCrossRefPubMedGoogle Scholar
  9. 9.
    Balamurugan V, Hemadri D, Gajendragad MR, Singh RK, Rahman H. Diagnosis and control of peste des petits ruminants: a comprehensive review. Virusdisease. 2014;25(1):39–56.PubMedCentralCrossRefPubMedGoogle Scholar
  10. 10.
    Brindha K, Raj GD, Ganesan PI, Thiagarajan V, Nainar AM, Nachimuthu K. Comparison of virus isolation and polymerase chain reaction for diagnosis of peste des petits ruminants. Acta Virol. 2001;45(3):169–72.PubMedGoogle Scholar
  11. 11.
    Chaudhary D, Muthuchelvan D, De A, Yadav AK, Sudhakar SB, Rajak KK, Pandey AB. Molecular characterization of PPR virus from an outbreak in Tripura reveals its origin from Bangladesh (2013). In: Asian-Pacific Congress of Virology (VIROCON 2013), Amity Institute of Virology and Immunology, Noida, UP, India from 17th to 20th Dec; p. 128.Google Scholar
  12. 12.
    Chavan VV, Digraskar SU, Dhonde SN, Bedarkar SN. Seromonitoring of peste des petits ruminants (PPR) in goats (Capra hircus) of Parbhani region of Maharashtra. Vet World. 2009;2:299–300.Google Scholar
  13. 13.
    Diallo A. Morbillivirus group: genome organisation and proteins. Vet Microbiol. 1990;23(1–4):155–63.CrossRefPubMedGoogle Scholar
  14. 14.
    Diallo A, Minet C, Le Goff C, et al. The threat of peste des petits ruminants: progress in vaccine development for disease control. Vaccine. 2007;25(30):5591–7.CrossRefPubMedGoogle Scholar
  15. 15.
    Forsyth MA, Barrett T. Evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petitsruminants viruses for epidemiological studies. Virus Res. 1995;39(2–3):151–63.CrossRefPubMedGoogle Scholar
  16. 16.
    Gargadennec L, Lalanne A. La peste des petits ruminants. Bulletin des Services Zootechniques, et des Epizooties de I’ Afrique Occidntale Francaise. 1942;5:16–21.Google Scholar
  17. 17.
    George AA. Comparative evaluation of different gene targets for PCR diagnosis of PPR. M.V.Sc., thesis submitted to Deemed University IVRI, Izatnagar, India; 2002.Google Scholar
  18. 18.
    Kerur N. Assessment of different gene targets for detection of peste des petits ruminants virus, by RT-PCR and sequence analysis of F and N gene Segments, M.V.Sc., thesis, Anand Agricultural University Anand, Gujarat; 2005.Google Scholar
  19. 19.
    Khan HA, Siddique M, Sajjad-ur-Rahman Abubakar M, Ashraf M. The detection of antibody against peste des petits ruminants virus in sheep, goats, cattle and buffaloes. Trop Anim Health Prod. 2008;40:521–7.CrossRefPubMedGoogle Scholar
  20. 20.
    Lefevre PC, Diallo A, Schenkel S, Hussein S, Staak G. Serological evidence of peste des petits ruminants in Jordan. Vet Rec. 1991;128:110.CrossRefPubMedGoogle Scholar
  21. 21.
    Majumder P. Studies on an incidence of peste des petits ruminants in the regional goat breeding farm at Debipur, Tripura. Indian J Anim Health. 1997;36:169–71.Google Scholar
  22. 22.
    Nanda YP, Chatterjee A, Purohit AK, Diallo A, Inui K, Sharma RN, et al. The isolation of pestedespetits ruminants virus from northern India. Vet Microbiol. 1995.Google Scholar
  23. 23.
    Nanda YP, Chatterjee A, Purohit AK, Diallo A, Innui K, Sharma RN, Libeau G, Thevasagayam JA, Bruning A, Kitching RP, Anderson J, Barrett T, Taylor WP. The isolation of peste des petits ruminants virus from Northern India. Vet Microbiol. 1996;51(3–4):207–16.CrossRefPubMedGoogle Scholar
  24. 24.
    Roeder PL, Abraham G, Kenfe G, Barrett T. PPR in Ethiopian goats. Trop Anim Health Prod. 1994;26:69–73.CrossRefPubMedGoogle Scholar
  25. 25.
    Shaila MS, Purushothaman V, Bhavasar D, Venugopal K, Venkatesan RA. Peste des petits ruminants in India. Vet Rec. 1989;125:602.PubMedGoogle Scholar
  26. 26.
    Shaila MS, Shamaki D, Forsyth MA, Diallo A, Goatley L, Kitching RP, Barrett T. Geographic distribution and epidemiology of peste des petitsruminants virus. Virus Res. 1996;43(2):149–53.CrossRefPubMedGoogle Scholar
  27. 27.
    Sing VP, Cham VK, Mondhe KS. Peste des petits ruminants: an outbreak in sheep in Rajasthan. India Vet J. 1996;73:466–7.Google Scholar
  28. 28.
    Singh RP, Saravanan P, Sreenivasa BP, Singh RK, Bandyopadhyay SK. Prevalence and distribution of peste des petits ruminants virus infection in small ruminants in India. Rev Sci Tech. 2004;23:807–19.PubMedGoogle Scholar
  29. 29.
    Singh RK, Balamurugan V, Bhanuprakash V, Sen A, Saravanan P, Yadav MP. Possible control and eradication of peste des petits ruminants from India: technical aspects. Vet Ital. 2009;45:449–62.PubMedGoogle Scholar
  30. 30.
    Tiwari A. Prevalence of pesti des petits ruminants (PPR) virus in small ruminants of Gujarat and its characterization by RT-PCR/RFLP and SSCP profile. M.V.Sc., thesis, Anand Agricultural University, Anand, India; 2004.Google Scholar
  31. 31.
    Zahur AB, Ullah A, Hussain M, Irshad H, Hameed A, Jahangir M, Farooq MS. Sero-epidemiology of peste des petits ruminants (PPR) in Pakistan. Prev Vet Med. 2011;102:87–92.CrossRefPubMedGoogle Scholar

Copyright information

© Indian Virological Society 2015

Authors and Affiliations

  • Maitrayee Devi
    • 1
    Email author
  • Sutopa Das
    • 1
  • Krishna Sharma
    • 1
  • Rupam Dutta
    • 2
  1. 1.Department of Veterinary Microbiology, College of Veterinary ScienceAssam Agricultural UniversityGuwahatiIndia
  2. 2.Department of Veterinary BiotechnologyIndian Veterinary Research InstituteIzatnagar, BareillyIndia

Personalised recommendations