Betanoda virus is an emerging problem in several marine fish species in various geographic areas all over the world. In recent years, mullets stock of the Caspian Sea decreased dramatically and a betanoda virus like-agent was introduced as the cause of mullet’s mortality. The main objective of the present study is to compare hemato-biochemical parameters in healthy and infected sharpnose mullets (Liza saliens) to betanoda virus like-agent. The adult sharpnose mullets (34 clinically affected fish + 34 apparently healthy fish) were captured from the southeast the Caspian Sea from 2012 to 2013. All of the captured fish were 4 or 5 years old. The main clinical sings of infected fish were lethargy, severe abdominal distention, abnormal swimming and hyperinflation of swim bladder. The results showed that the weight and total length of infected fish were significantly lower than the healthy fish. The results of histopatological evaluation and indirect florescent antibody test were confirmed the presence of a betanoda virus-like agent in infected fish. The diseased fish were severely anemic (hypochromic macrocytic anemia) and had a serious haemopoietic disorders. The anemia associated with a sever leukopenia, a significant rise of neutrophils and immature neutrophils and a significant decries of lymphocytes percentage. Total protein, albumin and total immunoglobulin levels were significantly reduced in the serum of infected fish, while the activities of aspartate aminotransferase and alanine aminotransferase significantly increased when compared to the healthy fish. These results suggested that the feeding of the infected fish to betanoda virus like-agent were disturbed and it could be cause haemato-biochemical disorder and mortality of the fish in Iranian water of the Caspian Sea.
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Adham KG, Hashem HO, Abu-Shaban MB, Kamel AH. Vitamin C deficiency in catfish Clarias gariepinus. Aquac Nutr. 2000;6:129–39.
Amend DF, Smith L. Pathophysiology of infectious hematopoietic necrosis virus disease in rainbow trout: hematological and blood chemical changes in moribund fish. Infect Immun. 1975;11:171–9.
Amini F. Biology of mullet species and their adaptation to fresh water. In: Proceedings of the national conference on fisheries resource management of the Caspian Sea, Babolsar, Iran; 1989. p. 73–9 (In Persion).
Arimoto M, Mori K, Nakai T, Muroga K, Furusawa I. Pathogenicity of the causative agent of viral nervous necrosis disease in striped jack, Pseudocaranx dentex (Bloch and Schneider). J Fish Dis. 1993;16:461–9.
Bancroft JD, Stevens A. Theory and practice of histological techniques. Edinburgh: Churchill Livingstone; 1990. p. 740.
Bekcan S, Dogankaya L, Cakirogullari GC. Growth and body composition of European catfish (Silurus glanis) fed diet containing different percentages of protein. Israeli J Aquac Bamidgeh. 2006;58:137–42.
Berg LS. Freshwater fishes of the U.S.S.R. and adjacent countries, 4th edn. Israel Program for Scientific Translations Ltd., (Russian version published in 1949), Jerusalem; 1965. p. 510.
Binaii M, Ghiasi M, Pourgholam R, Zorriehzahra J, Saidi AA, Behrozi S. Comparative evaluation of hematological factors of healthy and suspected to viral nervous necrosis mullets in Mazandaran province. In: Proceedings of the 16th Iranian Veterinary Congress, Tehran, Iran; 2010. p. 200–1 (In Persion).
Blaxhall PC, Daisley W. Routine haematological methods for use with fish blood. J Fish Biol. 1973;5:771–81.
Boonyaratpalin S, Supamattaya K, Kasornchandra J, Hoffman RW. Picorna-like virus associated with mortality and a spongiosus encephalopathy in grouper Epinephelus malabaricus. Dis Aquat Org. 1996;26:75–80.
Comps M, Trindade M, Delsert C. Investigation of fish encephalitis viruses (FEV) expression in marine fishes using DIG-labelled probes. Aquaculture. 1996;143:113–21.
Cuesta A, Meseguer J, Esteban MA. Total serum immunoglobulin M levels are affected by immunomodulators in seabream (Sparus aurata L.). Vet Immunol Immunopathol. 2004;101:203–10.
Davis CR, Marty CG, Adkison MA, Freiberg EF, Hedrick RP. Association of plasma IgM with body size, histopathologic changes and plasma chemistries in adult Pacific herring Clupea pallasi. Dis Aquat Org. 1999;38:125–33.
Dmitriev AN. Mullet in the Iranian waters of Caspian. Priroda 1964;12:74–5 (In Russian).
Fazli H, Ghaninejad D, Janbaza AA, Daryanabarda GR. Population ecology parameters and biomass of golden grey mullet (Liza auratus) in Iranian waters of the Caspian Sea. Fish Res. 2008;93:222–8.
Fazli H, Daryanabard GH, Abdolmaleki S, Bandani GA. Stock assessment and management implications of golden grey mullet (Liza aurata Risso, 1810) in Iranian waters of the Caspian Sea. J Appl Ichthyol. 2013;29:431–6.
Gillis TE, Ballantyne JS. The effect of starvation on plasma free amino acid and glucose concentration in lake sturgeon. J Fish Biol. 1996;49:1306–16.
Grohmann U, Bronte V. Control of immune response by amino acid metabolism. J Fish Biol. 2010;236:243–64.
Grotmoll S, TotlandK GK, Thorud K, Hjeltnes BK. Vacuolating encephalopathy and retinopathy associated with a nodavirus-like agent: a probable cause of mass mortality of cultured larval and juvenile Atlantic halibut Hippoglossus hippoglossus. Dis Aquat Anim. 1997;29:85–97.
Hung SSO, Liu W, Li H, Storebakken T, Cui Y. Effect of starvation on some morphological and biochemical parameters in white sturgeon, Acipenser transmontanus. Aquaculture. 1997;151:357–63.
Johansen R, Grove S, Svendsen AK, Modahl I, Dannevig BH. A sequential study of pathological findings in Atlantic halibut, Hippoglossus hippoglossus (L.), throughout one year after an acute outbreak of viral encephalopathy and retinopathy. J Fish Dis. 2004;27:327–41.
Lee RG, Foerster J, Jukens J, Paraskevas F, Greer JP, Rodgers GM. Wintrobe’sclinical hematology. 10th ed. New York: Lippincott, Williams & Wilkins; 1998. p. 1484.
Martins ML, Mouriño JLP, Amaral GV, Vieira FN, Dotta G, Jatobá AMB, Pedrotti FS, Jerônimo GT, Buglione-Neto CC, Pereira JG. Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. Braz J Biol. 2008;68:657–61.
Mori K, Nakai T, Nagahara M, Muroga K, Mekuchi T, Kanno T. A viral disease in hatchery-reared larvae and juveniles of redspotted grouper. Fish Pathol. 1991;26:209–10.
Munday BL, Langdon SJ, Hyatt A, Humphrey JD. Mass mortality associated with a viral-induced vacuolating encephalopathy and retinopathy of larval juvenile barramundi, Lates calcarifer Bloch. Aquaculture. 1992;103:197–211.
Munday BL, Kwang J, Moody N. Betanodavirus infections of teleost fish: a review. J Fish Dis. 2002;25:127–42.
Nelson JS. Fishes of the world. 4th ed. New York: Wiley; 2004. p. 624.
Nopadon P, Aranya P, Tipaporn T, Toshihiro N, Takayuki K, Masashi M, Makoto E. Nodavirus associated with pathological changes in adult spotted coralgroupers (Plectropomus maculatus) in Thailand with viral nervous necrosis. Res Vet Sci. 2009;87:97–101.
Office International des Epizooties. Viral encephalopathy and retinopathy. In: Manual of diagnostic test for aquatic animals, OIE, Paris; 2003. p. 136.
Park IS, Hur JW, Choi JW. Hematological responses, survival, and respiratory exchange in the olive flounder, Paralichthys olivaceus, during starvation. Asian Australas J Anim Sci. 2012;25:1276–84.
Pascual P, Pedrajas JR, Toribio F, López-Barea J, Peinado J. Effect of food deprivation on oxidative stress biomarkers in fish (Sparus aurata). Chem Biol Interact. 2003;145:191–9.
Patimar R. Some biological aspects of the sharpnose mullet Liza saliens (Risso, 1810) in Gorgan Bay-Miankaleh Wildlife Refuge (the Southeast Caspian Sea). Turk J Fish Aquat Sci. 2008;8:225–32.
Pérez A, Cardenete G, Hidalgo MC, Gracía-Alcázar A, Abellán E, Morales AE. Metabolic adjustments of Dentex dentex to prolonged starvation and re-feeding. Fish Physiol Biochem. 2012;38:1145–57.
Řehulka J. Aeromonas causes severe skin lesions rainbow trout (Oncorhynchus mykiss): clinical pathology, haematology and biochemistry. Acta Vet Brno. 2002;71:351–60.
Řehulka J. Haematological analyses in rainbow trout Oncorhynchus mykiss affected by viral haemorrhagic septicaemia (VHS). Dis Aquat Org. 2003;56:185–93.
Rios FS, Oba ET, Fernandes MN, Kalinin AL, Rantin FT. Erythrocyte senescence and haematological changes induced by starvation in neotropical fish Traira, Hoplias malabaricus (Characiformes, Erythrinidae). Comp Biochem Physiol. 2005;140:281–7.
Sakamoto S, Yone Y. Effect of starvation on hematological characteristics and the content of chemical components and activities of enzymes in blood serum of red sea bream. J Fac Agric Kyushu Univ. 1978;23:63–9.
Saksida SM, Thorburn MA, Speare DJ, Markham RJF, Kent ML. A field evaluation of an indirect immunofluorescent antibody test developed to diagnose plasmacytoid leukemia in Chinook Salmon (Oncorhynchus tshawytscha). Can J Vet Res. 1999;63:107–12.
Shahsavani D, Mohri M, Gholipour Kanani H. Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas. Fish Physiol Biochem. 2010;36:39–43.
Siwicki AK, Anderson DP. Nonspecific defense mechanisms assay in fish: II. Potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs and total immunoglobulin level in serum. In: Siwicki AK, Anderson DP, Waluga J, editors. Fish disease diagnosis and prevention methods. Poland: Olsztyn; 1993. p. 105–12.
Smith PA, Larenas J, Contreras J, Cassigoli J, Venegas C, Rojas ME, Guajardo A. Infectious haemolytic anaemia causes jaundice outbreaks in seawater-cultured coho salmon, Oncorhynchus kisutch (Walbaum), in Chile. J Fish Dis. 2006;29:709–15.
Soltani M, Rehanndeh M. Study of the bloat in golden grey mullet in the Caspian Sea. J Fac Vet Med Tehran Univ. 2001;56:1–3 (In Persion).
Soltani M, Ghasemi M, Sharifrohani M, Sharifpour I, Zorrieh Zahra J. Isolation and identification of betanodavirus causing mass mortalities in golden grey mullet (Liza auratus) in the Caspian Sea. Iran J Vet Res. 2010;4:201–8.
Sopińska A. Effect of physiological factors, stress and disease on hematological parameters of carp, with particular reference to leukocyte pattern. II. Hematological results of stress in carp. Acta Ichtyol Pisca. 1984;16:121–39.
Stoskof MK. Fish medicine. Philadelphia: W. B. Saunders Company; 1993.
Tm C, Dove ADM, Arnold JE. Hematologic disorders of fish. Vet Clin Anim. 2008;11:445–64.
Uribe C, Folch H, Enriquez R, Moran G. Innate and adaptive immunity in teleost fish: a review. Vet Med. 2011;10:445–62.
Waagbø R, Sandnes K, Espelid S, Lie Ø. Haematological and biochemical analyses of Atlantic salmon Salmo salar L., suffering from cold water vibriosis (Hitra disease). J Fish Dis. 1988;4:417–23.
Yoshikoshi K, Inoue K. Viral nervous necrosis in hatchery—reared larvae and juveniles of Japanese parrot fish Oplegnathus fasciatus. J Fish Dis. 1990;13:69–77.
Zar JH. Biostatistical analysis. New Jersey: Prentice-Hall; 1994. p. 662.
Zorriehzahra MJ, Nakai T, Sharifpour I, Gomez DG, Chi CS, Soltani M, Hassan HMD, Sharifrohani M, Saidi AA. Mortality of wild golden grey mullet (Liza auratus) in Iranian waters of the Caspian Sea, associated with viral nervous necrosis-like agent. Iran J Fish Sci. 2005;4:43–58.
Zorriehzahra MJ, Nazari A, Ghasemi M, Ghiasi M, Haghighi Karsidani S, Giuseppe B, Mohd Daud HH. Vacuolating encephalopathy and retinopathy associated with a nodavirus-like agent: a probable cause of mass mortality of wild Golden grey mullet (Liza aurata) and Sharpnose grey mullet (Liza saliens) in Iranian waters of the Caspian Sea. J Virus Dis. 2014;25(4):430–6.
The authors wish to special thank Mr. Sajad Asgharikhah, Mr. Hossain Taleshian, Mr. Faramarz Bagherzadeha and the staff of stock assessment department of Caspian Sea Ecology Research Center for their kind help during the experiment.
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Ghiasi, M., Binaii, M., Ghasemi, M. et al. Haemato-biochemical disorders associated with nodavirus like-agent in adult leaping mullet Liza saliens (Risso, 1810) in the Caspian Sea. VirusDis. 27, 12–18 (2016). https://doi.org/10.1007/s13337-015-0289-1
- Liza saliens
- Biochemical changes
- Caspian Sea