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Isolation and phylogenetic characterization of Canine distemper virus from India

VirusDisease volume 26, pages 133–140 (2015)Cite this article

Abstract

Canine distemper (CD), caused by canine distemper virus (CDV) is a highly contagious disease that infects a variety of carnivores. Sequence analysis of CDVs from different geographical areas has shown a lot of variation in the genome of the virus especially in haemagglutinin gene which might be one of the causes of vaccine failure. In this study, we isolated the virus (place: Ludhiana, Punjab; year: 2014) and further cloned, sequenced and analyzed partial haemagglutinin (H) gene and full length genes for fusion protein (F), phosphoprotein (P) and matrix protein (M) from an Indian wild-type CDV. Higher sequence homology was observed with the strains from Switzerland, Hungary, Germany; and lower with the vaccine strains like Ondersteport, CDV3, Convac for all the genes. The multiple sequence alignment showed more variation in partial H (45 nucleotide and 5 amino acid substitutions) and complete F (79 nucleotide and 30 amino acid substitutions) than in complete P (44 nucleotide and 22 amino acid substitutions) and complete M (22 nucleotide and 4 amino acid substitutions) gene/protein. Predicted potential N-linked glycosylation sites in H, F, M and P proteins were similar to the previously known wild-type CDVs but different from the vaccine strains. The Indian CDV formed a distinct clade in the phylogenetic tree clearly separated from the previously known wild-type and vaccine strains.

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Acknowledgments

This work was funded by School of Animal Biotechnology, GADVASU, Ludhiana, Punjab. Jawaharlal Nehru Memorial Fund is highly acknowledged for providing Jawaharlal Nehru Scholarship for Doctoral Studies to Swati.

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Affiliations

  1. School of Animal Biotechnology, GADVASU, Ludhiana, 141004, India

    Swati, Dipak Deka & Ramneek Verma

  2. Department of Veterinary Medicine, GADVASU, Ludhiana, 141004, India

    Sanjeev Kumar Uppal

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  1. Swati
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  2. Dipak Deka
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  3. Sanjeev Kumar Uppal
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  4. Ramneek Verma
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Correspondence to Dipak Deka.

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Swati, Deka, D., Uppal, S.K. et al. Isolation and phylogenetic characterization of Canine distemper virus from India. VirusDis. 26, 133–140 (2015). https://doi.org/10.1007/s13337-015-0256-x

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  • DOI: https://doi.org/10.1007/s13337-015-0256-x

Keywords

  • Canine distemper virus
  • Indian strain
  • Phylogenetic analysis
  • Genotype
  • N-Linked glycosylation