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Characterization of cytopathogenicity of classical swine fever virus isolate induced by Newcastle disease virus

Abstract

Classical swine fever virus (CSFV), the causative agent of classical swine fever, belongs to the family Flaviviridae and genus Pestivirus. Some pestiviruses exhibit cytopathic effect in cell culture but exact phenomenon is unknown. Over expression of NS2-3 gene, presence of defective interfering particle and exaltation of Newcastle disease virus (END) phenomenon could be the reasons of cytopathogenicity. In the present study, a CSFV isolate exhibiting cytopathic effect (CPE) in Madin-Darby Canine Kidney (MDCK) cell line was characterized. To characterize cytopathogenicity of such isolate, END test was carried out. Interference of Newcastle disease virus (NDV) in MDCK adapted CSFV was confirmed by RT-PCR and virus neutralization test. Absence of CPE and NDV specific nucleic acid after neutralization confirmed the induction of CPE by NDV. Further, identity of the CSFV isolate in MDCK cell line by immunoperoxidase test, immunoblotting and RT-PCR post NDV neutralization established the virus replication without CPE (non-cytopathic isolate). Findings suggest that, there could be a chance of mixed infection of both CSFV and NDV in the piglet from which the sample was collected for virus isolation.

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Acknowledgments

The authors are grateful to Director of the Institute for providing the necessary facilities to carry out this work. Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, New Delhi is highly acknowledged for providing necessary funds under DBT-CSF network project to carry out this study.

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Correspondence to K. K. Rajak.

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Raut, S.D., Rajak, K.K., Kumar, R. et al. Characterization of cytopathogenicity of classical swine fever virus isolate induced by Newcastle disease virus. VirusDis. 26, 70–76 (2015). https://doi.org/10.1007/s13337-015-0253-0

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Keywords

  • CSF
  • Virus
  • Cytopathogenicity
  • END phenomenon
  • VNT
  • MDCK