, Volume 26, Issue 1–2, pp 9–18 | Cite as

Gene expression profiling in gill tissues of White spot syndrome virus infected black tiger shrimp Penaeus monodon by DNA microarray

  • M. S. ShekharEmail author
  • A. Gomathi
  • G. Gopikrishna
  • A. G. Ponniah
Original Article


White spot syndrome virus (WSSV) continues to be the most devastating viral pathogen infecting penaeid shrimp the world over. The genome of WSSV has been deciphered and characterized from three geographical isolates and significant progress has been made in developing various molecular diagnostic methods to detect the virus. However, the information on host immune gene response to WSSV pathogenesis is limited. Microarray analysis was carried out as an approach to analyse the gene expression in black tiger shrimp Penaeus monodon in response to WSSV infection. Gill tissues collected from the WSSV infected shrimp at 6, 24, 48 h and moribund stage were analysed for differential gene expression. Shrimp cDNAs of 40,059 unique sequences were considered for designing the microarray chip. The Cy3-labeled cRNA derived from healthy and WSSV-infected shrimp was subjected to hybridization with all the DNA spots in the microarray which revealed 8,633 and 11,147 as up- and down-regulated genes respectively at different time intervals post infection. The altered expression of these numerous genes represented diverse functions such as immune response, osmoregulation, apoptosis, nucleic acid binding, energy and metabolism, signal transduction, stress response and molting. The changes in gene expression profiles observed by microarray analysis provides molecular insights and framework of genes which are up- and down-regulated at different time intervals during WSSV infection in shrimp. The microarray data was validated by Real Time analysis of four differentially expressed genes involved in apoptosis (translationally controlled tumor protein, inhibitor of apoptosis protein, ubiquitin conjugated enzyme E2 and caspase) for gene expression levels. The role of apoptosis related genes in WSSV infected shrimp is discussed herein.


Microarray WSSV Penaeus monodon 



Authors acknowledge Department of Biotechnology, Ministry of Science and Technology, India for funding this study under the project “Molecular studies on sequential pathogenesis of WSSV and defence mechanism in Penaeus monodon”.

Supplementary material

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Supplementary material 1 (PDF 5 kb)
13337_2014_243_MOESM2_ESM.doc (83 kb)
Supplementary material 2 (DOC 83 kb)


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Copyright information

© Indian Virological Society 2015

Authors and Affiliations

  • M. S. Shekhar
    • 1
    Email author
  • A. Gomathi
    • 1
  • G. Gopikrishna
    • 1
  • A. G. Ponniah
    • 1
  1. 1.Genetics and Biotechnology UnitCentral Institute of Brackishwater AquacultureChennaiIndia

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