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Molecular detection and characterization of infectious laryngotracheitis virus (Gallid herpesvirus-1) from clinical samples of commercial poultry flocks in India

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Abstract

Although the existence of infectious laryngotracheitis virus (ILTV) in India was first reported in 1964, no reports are available regarding its molecular detection and characterization. The present study was aimed to detect and characterize ILTV from recent respiratory disease complex (RDC) outbreaks of commercial poultry flocks in different parts of the country by using envelope glycoprotein G gene (US4 gene) based PCR and sequencing. A total of thirty two flocks with a history of RDC were investigated. Overall, all the strains/breeds of birds and all ages of birds are equally susceptible and depending on the severity, the clinical signs and gross lesions were varied. Out of 32 flocks investigated 10 were found positive for ILTV infection by PCR. The phylogenetic analyses of eight representative sequences in the present study deciphered that Indian ILT viruses are closely related to chicken embryo origin vaccine strains of Italy, USA, China and Brazil.

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References

  1. Alexander HS, Nagy E. Polymerase chain reaction to detect infectious laryngotracheitis virus in conjunctival swabs from experimentally infected chickens. Avian Dis. 1997;41:646–53.

    Article  CAS  PubMed  Google Scholar 

  2. Bagust TJ, Jones RC, Guy JS. Avian infectious laryngotracheitis. Rev Off Int Epizoot. 2000;19:483–92.

    CAS  PubMed  Google Scholar 

  3. Blacker HP, Kirkpatrick NC, Rubite A, O’Rourke D, Noormohammadi AH. Epidemiology of recent outbreaks of infectious laryngotracheitis in poultry in Australia. Aust Vet J. 2011;89:89–94.

    Article  CAS  PubMed  Google Scholar 

  4. Bradbury JM. Avian mycoplasma infections: prototype of mixed infections with mycoplasmas, bacteria and viruses. Ann Microbiol (Paris). 1984;135A:83–9.

    CAS  Google Scholar 

  5. Crawshaw GJ, Boycott BR. Infectious laryngotracheitis in peafowl and pheasants. Avian Dis. 1982;26:397–401.

    Article  CAS  PubMed  Google Scholar 

  6. Davison AJ, Eberle R, Ehlers B, Hayward GS, McGeoch DJ, Minson AC. The order Herpesvirales. Arch Virol. 2009;154:171–7.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Dufour-Zavala L. Epizootiology of infectious laryngotracheitis and presentation of an industry control program. Avian Dis. 2008;52:1–7.

    Article  PubMed  Google Scholar 

  8. Fuchs W, Klupp BG, Granzow H, Osterrieder N, Mettenleiter TC. The interacting UL31 and UL34 gene products of pseudorabies virus are involved in egress from the host-cell nucleus and represent components of primary enveloped but not mature virions. J Virol. 2002;76:364–78.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Fuchs W, Veits J, Helferich D, Granzow H, Teifke JP, Mettenleiter TC. Molecular biology of avian infectious laryngotracheitis virus. Vet Res. 2007;38:261–79.

    Article  CAS  PubMed  Google Scholar 

  10. Georgiades G, Iordanidis P, Koumbati M. Cases of swollen head syndrome in broiler chickens in Greece. Avian Dis. 2001;45:745–50.

    Article  CAS  PubMed  Google Scholar 

  11. Gough AW, Pettit JR, Gagnon A, Weber LJ. An outbreak of infectious laryngotracheitis in commercial poultry flocks in Ontario. Can J Comp Med. 1977;41:146–51.

    CAS  PubMed Central  PubMed  Google Scholar 

  12. Guy JS, Garcia M. Laryngotracheitis. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, editors. Diseases of poultry. 12th ed. Ames, Iowa: Blackwell Pub. Professional; 2008. p. 137–52.

    Google Scholar 

  13. Hayles LB, Macdonald KR, Newby WC, Wood CW, Gilchrist EW, MacNeill AC. Epizootiology of infectious laryngotracheitis in British Columbia 1971–1973. Can Vet J. 1976;17:101–8.

    CAS  PubMed Central  PubMed  Google Scholar 

  14. Islam MS, Khan MSR, Islam MA, Hassan J. Isolation and characterization of infectious laryngotracheitis virus in layer chickens. Bangladesh J Vet Med. 2010;8:123–30.

    Google Scholar 

  15. Jahan MS, Khan MFR, Nazir KNH, Amin MM, Rahman MB. Sero-Surveillance of infectious laryngotracheitis in layer birds in bangladesh. Microbes and Health [Internet]. 2013 [cited 2013 Oct 16];1. http://banglajol.info/index.php/MH/article/view/14086.

  16. Jones RC. Infectious laryngotracheitis. In: Pattison M, McMullin P, Bradbury JM, Alexander D, editors. Poultry diseases. 6th ed. Edinburgh: Elsevier; 2008. p. 267–71.

    Google Scholar 

  17. Jordan FT. The epidemiology of disease of multiple aetiology: the avian respiratory disease complex. Vet Rec. 1972;90:556–62.

    Article  CAS  PubMed  Google Scholar 

  18. Kirkpatrick NC, Mahmoudian A, Colson CA, Devlin JM, Noormohammadi AH. Relationship between mortality, clinical signs and tracheal pathology in infectious laryngotracheitis. Avian Pathol. 2006;35:449–53.

    Article  CAS  PubMed  Google Scholar 

  19. Leib DA, Bradbury JM, Hart CA, McCarthy K. Genome isomerism in two alphaherpes viruses: Herpesvirus saimiri-1 (Herpesvirus tamarinus) and avian infectious laryngotracheitis virus. Brief Report Arch Virol. 1987;93:287–94.

    Article  CAS  Google Scholar 

  20. Malik YS, Patnayak DP, Goyal SM. Detection of three avian respiratory viruses by single-tube multiplex reverse transcription-polymerase chain reaction assay. J Vet Diagn Invest. 2004;16:244–8.

    Article  PubMed  Google Scholar 

  21. Neff C, Sudler C, Hoop RK. Characterization of western European field isolates and vaccine strains of avian infectious laryngotracheitis virus by restriction fragment length polymorphism and sequence analysis. Avian Dis. 2008;52:278–83.

    Article  CAS  PubMed  Google Scholar 

  22. Ojkic D, Swinton J, Vallieres M, Martin E, Shapiro J, Sanei B, et al. Characterization of field isolates of infectious laryngotracheitis virus from Ontario. Avian Pathol. 2006;35:286–92.

    Article  CAS  PubMed  Google Scholar 

  23. Oldoni I, García M. Characterization of infectious laryngotracheitis virus isolates from the US by polymerase chain reaction and restriction fragment length polymorphism of multiple genome regions. Avian Pathol. 2007;36:167–76.

    Article  CAS  PubMed  Google Scholar 

  24. Oldoni I, Rodríguez-Avila A, Riblet S, García M. Characterization of infectious laryngotracheitis virus (ILTV) isolates from commercial poultry by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Avian Dis. 2008;52:59–63.

    Article  PubMed  Google Scholar 

  25. Ottiger H-P. Development, standardization and assessment of PCR systems for purity testing of avian viral vaccines. Biologicals. 2010;38:381–8.

    Article  CAS  PubMed  Google Scholar 

  26. Pang Y, Wang H, Girshick T, Xie Z, Khan MI. Development and application of a multiplex polymerase chain reaction for avian respiratory agents. Avian Dis. 2002;46:691–9.

    Article  PubMed  Google Scholar 

  27. Rea TJ, Timmins JG, Long GW, Post LE. Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells. J Virol. 1985;54:21–9.

    CAS  PubMed Central  PubMed  Google Scholar 

  28. Roussan DA, Haddad R, Khawaldeh G. Molecular survey of avian respiratory pathogens in commercial broiler chicken flocks with respiratory diseases in Jordan. Poult Sci. 2008;87:444–8.

    Article  CAS  PubMed  Google Scholar 

  29. Singh SB, Singh GR, Singh CM. A preliminary report on the occurrence of infectious laryngotracheitis of poultry in India. Poult Sci. 1964;43:492–4.

    Article  Google Scholar 

  30. Srinivasan P, Balachandran C, Gopalakrishna Murthy TR, Saravanan S, Pazhanivel N, Mohan B. Pathology of infectious laryngotracheitis in commercial layer chicken. Indian Vet J. 2012;89.

  31. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  32. Wild MA, Cook S, Cochran M. A genomic map of infectious laryngotracheitis virus and the sequence and organization of genes present in the unique short and flanking regions. Virus Genes. 1996;12:107–16.

    Article  CAS  PubMed  Google Scholar 

  33. Williams RA, Bennett M, Bradbury JM, Gaskell RM, Jones RC, Jordan FT. Demonstration of sites of latency of infectious laryngotracheitis virus using the polymerase chain reaction. J Gen Virol. 1992;73(Pt 9):2415–20.

    Article  PubMed  Google Scholar 

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Acknowledgments

Authors are thankful to the Director IVRI for providing facilities and funds for carrying out the study.

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Authors and Affiliations

  1. Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, 243 122, Uttar Pradesh, India

    Vasudevan Gowthaman, Sambhu Dayal Singh, Kuldeep Dhama & Rajamani Barathidasan

  2. Poultry Disease Diagnosis and Surveillance Laboratory, Veterinary College and Research Institute Campus, Namakkal, 637 002, Tamil Nadu, India

    Palani Srinivasan & Sellappan Saravanan

  3. Division of Virology, Indian Veterinary Research Institute, Muktheswar, 263 138, Uttarakhand, India

    Basavaraj S. Mathapati & Muthannan Andavar Ramakrishnan

Authors
  1. Vasudevan Gowthaman
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  2. Sambhu Dayal Singh
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  3. Kuldeep Dhama
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  4. Rajamani Barathidasan
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  5. Basavaraj S. Mathapati
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  6. Palani Srinivasan
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  7. Sellappan Saravanan
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  8. Muthannan Andavar Ramakrishnan
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Correspondence to Muthannan Andavar Ramakrishnan.

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Gowthaman, V., Singh, S.D., Dhama, K. et al. Molecular detection and characterization of infectious laryngotracheitis virus (Gallid herpesvirus-1) from clinical samples of commercial poultry flocks in India. VirusDis. 25, 345–349 (2014). https://doi.org/10.1007/s13337-014-0206-z

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  • DOI: https://doi.org/10.1007/s13337-014-0206-z

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