Abstract
Rotavirus (RV) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. Human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. Therefore, the present study was designed to explore RV incidence in a single approach from human and animal neonates sharing similar environment. Altogether, 200 diarrheal samples from children (50), piglets (80) and calves (70) were collected during the year of 2010–2012 from various locality, farms and hospitals, initially screened through monoclonal antibody based enzyme immunoassay followed by RNA-PAGE and VP7 gene amplification by Reverse transcription PCR. The overall prevalence of rotavirus was found to be 41.5 % (83/200) where maximum numbers of positive cases were found in piglets (46.3 %) followed by human (40 %) and cow (37.1 %). Majority of samples demonstrated characteristic group A rotavirus (RVA) electropherotype of 4:2:3:2 pattern. Moreover, RNA profiles of seven samples from piglets and calves revealed variation in the migration pattern of class II, III and class IV segments. The study, for the first time from the valley, detected 43.7 % of neonatal RVA positive cases from human and animal sharing similar setting. The variation in RNA migration pattern in seven cases signifies tentative cases of gene re-assortment that warrant further evaluation.
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Ahmed S, Hussain M, Akhter S, Islam T, Ahmed SU, Kabir ML. Genotypes of rotavirus diarrhoea in a children hospital of Bangladesh. Mymensingh Med J. 2012;21:497–502.
Aminu M, Page NA, Ahmad AA, Umoh JU, Dewar J, Steele AD. Diversity of rotavirus VP7 and VP4 genotypes in Northwestern Nigeria. J Infect Dis. 2010;202(Suppl):S198–204.
Armah GE, Hoshino Y, Santos N, Binka F, Damanka S, Adjei R, Honma S, Tatsumi M, Manful T, Anto F. The global spread of rotavirus G10 strains: Detection in Ghanaian children hospitalized with diarrhea. J Infect Dis. 2010;202(Suppl):S231–8.
Badaracco A, Garaicoechea L, Rodriguez D, Uriarte EL, Odeon A, Bilbao G, Galarza R, Abdala A, Fernandez F, Parreno V. Bovine rotavirus strains circulating in beef and dairy herds in Argentina from 2004 to 2010. Vet Microbiol. 2012;158:394–9.
Bozdayi G, Dogan B, Dalgic B, Bostanci I, Sari S, Battaloglu NO, Rota S, Dallar Y, Nishizono A, Nakagomi O, Ahmed K. Diversity of human rotavirus G9 among children in Turkey. J Med Virol. 2008;80:733–40.
Broor S, Ghosh D, Mathur P. Molecular epidemiology of rotaviruses in India. Indian J Med res. 2003;118:59–67.
Chouikha A, Fredj MB, Fodha I, Mathlouthi I, Ardhaoui M, Teleb N, Brini I, Messaadi F, Mastouri M, Sfar T, Hachicha M, Kammoun T, Bouaaziz A, Amri F, Harbi A, Zribi M, Bousnina S, Khemakhem A, Boujaafar N, Trabelsi A, Steele AD. Evolution of group A rotavirus strains circulating in Tunisia over a 3-year period (2005–2007). Pathol-biol. 2011;59:e79–83.
Desselberger U, Iturriza-Gomara M, Gray JJ. Rotavirus epidemiology and surveillance. Novartis Found Symp. 2001;238:125–47 discussion 147–152.
Falcone E, Tarantino M, Di Trani L, Cordioli P, Lavazza A, Tollis M. Determination of bovine rotavirus G and P serotypes in Italy by PCR. J Clin Microbiol. 1999;37:3879–82.
Ganime AC, Carvalho Costa FA, Mendonca MC, Vieira CB, Santos M, Costa Filho R, Miagostovich MP, Leite JP. Group A rotavirus detection on environmental surfaces in a hospital intensive care unit. Am J Infect Control. 2012;40:544–7.
Gatti MS, Ferraz MM, Racz ML, de Castro AF. Rotavirus excretion in naturally infected pigs with and without diarrhoea. Vet Microbiol. 1993;37:187–90.
Ghosh S, Samajdar S, Sinha M, Kobayashi N, Taniguchi K, Naik TN. Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11-like VP6 genes in G15P[21] strains. Virus Genes. 2008;37:241–9.
Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, Fang ZY. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990;28:276–82.
Gunn L, Feeney SA, Cashman O, Collins PJ, Coyle PV, O’Shea H. Molecular characterization of group A rotavirus found in elderly patients in Ireland; Predominance of G1P[8], continued presence of G9P[8], and emergence of G2P[4]. J Med Virol. 2012;84:2008–17.
Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982;16:473–7.
Jindal SR, Maiti NK, Oberoi MS. Genomic diversity and prevalence of Rotavirus in cow and buffalo calves in northern India. Rev Sci Tech. 2000;19:871–6.
Kirkwood CD. Genetic and antigenic diversity of human rotaviruses: potential impact on vaccination programs. J Infect Dis. 2010;202(Suppl):S43–8.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–5.
Martella V, Ciarlet M, Banyai K, Lorusso E, Arista S, Lavazza A, Pezzotti G, Decaro N, Cavalli A, Lucente MS, Corrente M, Elia G, Camero M, Tempesta M, Buonavoglia C. Identification of group A porcine rotavirus strains bearing a novel VP4 (P) Genotype in Italian swine herds. J Clin Microbiol. 2007;45:577–80.
Matthijnssens J, De Grazia S, Piessens J, Heylen E, Zeller M, Giammanco GM, Banyai K, Buonavoglia C, Ciarlet M, Martella V, Van Ranst M. Multiple reassortment and interspecies transmission events contribute to the diversity of feline, canine and feline/canine-like human group A rotavirus strains. Infect Genet Evol. 2011;11:1396–406.
Miyazaki A, Kuga K, Suzuki T, Kohmoto M, Katsuda K, Tsunemitsu H. Genetic diversity of group A rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: identification of a porcine rotavirus strain bearing a novel VP7 genotype, G26. Vet Res. 2011;42:112.
Mukherjee A, Nayak MK, Roy T, Ghosh S, Naik TN, Kobayashi N, Chawla Sarkar M. Detection of human G10 rotavirus strains with similarity to bovine and bovine-like equine strains from untypable samples. Infect Genet Evol. 2012;12:467–70.
Neog BK, Barman NN, Bora DP, Dey SC, Chakraborty A. Experimental infection of pigs with group A rotavirus and enterotoxigenic Escherichia coli in India: gross, histopathological and immunopathological study. Vet Ital. 2011;47:117–28.
Ramos AP, Stefanelli CC, Linhares RE, de Brito BG, Santos N, Gouvea V, de Cassia Lima R, Nozawa C. The stability of porcine rotavirus in feces. Vet Microbiol. 2000;71:1–8.
Rao CD, Gowda K, Reddy BS. Sequence analysis of VP4 and VP7 genes of nontypeable strains identifies a new pair of outer capsid proteins representing novel P and G genotypes in bovine rotaviruses. Virology. 2000;276:104–13.
Soeorg H, Tamm E, Huik K, Pauskar M, Magi D, Pruudel K, Vainomae L, Moosar L, Kirss K, Torm S, Narska M, Putsepp A, Nurm H, Pruunsild K, Janes A, Zilmer K, Lutsar I. Group A rotavirus genotypes circulating prior to implementation of a National Immunization Program in Estonia. Hum vaccin immunother. 2012;8:465–9.
Svensson L, Uhnoo I, Grandien M, Wadell G. Molecular epidemiology of rotavirus infections in Uppsala, Sweden, 1981: disappearance of a predominant electropherotype. J Med Virol. 1986;18:101–11.
Tatte VS, Chitambar SD. Diversity in the VP7 encoding genes of rotavirus strains isolated from adolescent and adult cases of acute gastroenteritis. J Med Virol. 2012;84:1481–8.
Acknowledgments
The authors gratefully acknowledge the Head, Dept of Microbiology, College of Veterinary Science, Assam Agricultural University for providing standard reference strain of rotavirus. Authors are also very grateful to the patients and animal dwellers for their willingness to participate and to provide information about them and their animals.
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Sharma, R., Bora, D.P., Chakraborty, P. et al. Circulation of group A rotaviruses among neonates of human, cow and pig: study from Assam, a north eastern state of India. Indian J. Virol. 24, 250–255 (2013). https://doi.org/10.1007/s13337-013-0153-0
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DOI: https://doi.org/10.1007/s13337-013-0153-0