Abstract
Coat protein (CP) and RNA3 from Prunus necrotic ringspot virus (PNRSV-rose), the most prevalent virus infecting rose in India, were characterized and regions in the coat protein important for self-interaction, during dimer formation were identified. The sequence analysis of CP and partial RNA 3 revealed that the rose isolate of PNRSV in India belongs to PV-32 group of PNRSV isolates. Apart from the already established group specific features of PV-32 group member’s additional group-specific and host specific features were also identified. Presence of methionine at position 90 in the amino acid sequence alignment of PNRSV CP gene (belonging to PV-32 group) was identified as the specific conserved feature for the rose isolates of PNRSV. As protein–protein interaction plays a vital role in the infection process, an attempt was made to identify the portions of PNRSV CP responsible for self-interaction using yeast two-hybrid system. It was found (after analysis of the deletion clones) that the C-terminal region of PNRSV CP (amino acids 153–226) plays a vital role in this interaction during dimer formation. N-terminal of PNRSV CP is previously known to be involved in CP-RNA interactions, but our results also suggested that N-terminal of PNRSV CP represented by amino acids 1–77 also interacts with C-terminal (amino acids 153–226) in yeast two-hybrid system, suggesting its probable involvement in the CP–CP interaction.
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Acknowledgments
The authors are thankful to the Director IHBT and Vice Chancellor, Shoolini University for providing research facilities. Authors would like to dedicate this paper to Late Dr A. A. Zaidi, a renowned virologist and mentor of Dr Saurabh Kulshrestha.
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Dr A. A. Zaidi—Deceased
The sequences reported in the paper were submitted to the EMBL data base with following accession numbers: PNRSV CP (AJ619958), PNRSV MP (AJ697737), PNRSV RNA 3 (AJ969095).
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Kulshrestha, S., Hallan, V., Sharma, A. et al. Molecular characterization and intermolecular interaction of coat protein of Prunus necrotic ringspot virus: implications for virus assembly. Indian J. Virol. 24, 235–241 (2013). https://doi.org/10.1007/s13337-013-0140-5
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DOI: https://doi.org/10.1007/s13337-013-0140-5