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Molecular Evidence for the Occurrence of Abutilon mosaic virus, A New World Begomovirus in India

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Abstract

During an investigation in the year 2010, on the weed reservoir of begomovirus, Abutilon pictum showing bright yellow mosaic symptoms was observed in Udhagamandalam, Tamil Nadu, India. The complete bipartite genome of a begomovirus was cloned and sequenced which revealed association of Abutilon mosaic virus (AbMV). Nicotiana benthamiana plants inoculated biolistically with the concatemers generated through rolling circle amplification of the cloned DNAs were asymptomatic; however three out of nine plants showed presence of viral DNA A. A recombination event in the ORF BC1 with ToLCNDV DNA B (HM989846) was detected. This is the first molecular evidence of AbMV in India.

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Acknowledgments

Authors are grateful for the facilities provided by The Director, Indian Agricultural Research Institute and Head, Division of Plant Pathology for conducting this study. The financial support by Department of Biotechnology, Government of India under the project: BT/PR9631/AGR/02/468/2007 is duly acknowledged. Support of National Phytotron Facility is highly appreciated.

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Authors and Affiliations

  1. Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute (IARI), New Delhi, 110012, India

    P. Jyothsna, Q. M. I. Haq & V. G. Malathi

  2. Plant Breeding, IARI, Regional Station, Wellington, 643231, India

    P. Jayaprakash

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  1. P. Jyothsna
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  2. Q. M. I. Haq
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  3. P. Jayaprakash
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  4. V. G. Malathi
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Correspondence to V. G. Malathi.

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Jyothsna, P., Haq, Q.M.I., Jayaprakash, P. et al. Molecular Evidence for the Occurrence of Abutilon mosaic virus, A New World Begomovirus in India. Indian J. Virol. 24, 284–288 (2013). https://doi.org/10.1007/s13337-013-0139-y

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