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Survey and RT-PCR Based Detection of Cardamom mosaic virus Affecting Small Cardamom in India

Indian Journal of Virology volume 21, pages 148–150 (2010)Cite this article

Abstract

Mosaic or marble or katte disease caused by Cardamom mosaic virus (CdMV) is an important production constraint in all cardamom growing regions of the world. In the present study, 84 cardamom plantations in 44 locations of Karnataka and Kerala were surveyed. The incidence of the disease ranged from 0 to 85%. The incidence was highest in Madikeri (Karnataka) while no incidence was recorded in Peermade (Kerala). In general, incidence and severity of the disease was higher in cardamom plantations of Karnataka. A procedure for total RNA isolation from cardamom and detection of CdMV through reverse transcription-polymerase chain reaction (RT-PCR) using primers targeting the conserved region of coat protein was standardized and subsequently validated by testing more than 50 field cardamom samples originating from Karnataka and Kerala states. The method can be used for indexing the planting material and identifying resistant lines/cultivars before either they are further multiplied in large scale or incorporated in breeding.

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References

  1. Bhat AI, Siju S. Development of a single-tube multiplex RT–PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper. Curr Sci. 2007;93:973–6.

    CAS  Google Scholar 

  2. Gonsalves D, Trujillo E, Hoch HC. Purification and some properties of a virus associated with cardamom mosaic, a new member of Potyvirus group. Plant Dis. 1986;70:65–9.

    Article  Google Scholar 

  3. Govindaraju C, Venugopal MN, Sudharsan MR. An appraisal of ‘kokke kandu’ - a new viral disease of cardamom and katte (Mosaic) disease in Karnataka. J Plant Crops. 1994;22:57–9.

    Google Scholar 

  4. Jacob T, Jebasingh T, Venugopal MN, Usha R. High genetic diversity in the coat protein and 3′ untranslated regions among the geographical isolates of Cardamom mosaic virus from South India. J Biosci. 2003;28(5):589–95.

    PubMed  Article  CAS  Google Scholar 

  5. Mayne WW. Report on cardamom cultivation in South India. ICAR Bull. 1951;50:62.

    Google Scholar 

  6. Naidu R, Venugopal MN, Rajan P. Investigations on strainal variation, epidemiology and characterization of ‘katte’ virus agent of small cardamom. Final report of research project. Kasargod: Central Plantation Crops Research Institute; 1985.

    Google Scholar 

  7. Naidu R, Venugopal MN. Epidemiology of katte virus disease of small cardamom II. Foci of primary disease entry, patterns and gradients of disease entry and spread. J Plant Crops. 1989;16:267–71.

    Google Scholar 

  8. Rao DG. Katte disease of cardamom and its control. Indian J Horticult. 1977;34:184–7.

    Google Scholar 

  9. Ravindran PN. Introduction. In: Ravindran PN, Madhusoodanan KJ, editors. Cardamom-The genus Elettaria. London and New York: Taylor and Francis; 2002. p. 1–10.

    Chapter  Google Scholar 

  10. Saigopal DVR, Naidu R, Joseph T. Early detection of ‘Katte’ disease of small cardamom through Enzyme Linked Immunosorbent assay (ELISA). J Plant Crops. 1992;20:73–5.

    Google Scholar 

  11. Siddapaji C, Reddy DNRN. A note on the occurrence of the aphid (Pentalonia nigronervosa f. caladii Van der Goot (Aphididae: Hemiptera) on cardamom (Elettaria cardamomum Maton). Mysore J Agric Sci. 1972;6:192–5.

    Google Scholar 

  12. Varma PM. Control of katte or mosaic disease of cardamom in North Kanara. Arecanut J. 1962;13:79–88.

    Google Scholar 

  13. Venugopal MN. Viral diseases of cardamom (Elettaria cardamomum Maton) and their management. J Spices Aromatic Crops. 1995;4:32–9.

    Google Scholar 

  14. Venugopal MN. Viral diseases of cardamom. In: Ravindran PN, Madhusoodanan KJ, editors. Cardamom- The genus Elettaria. New York: Taylor and Francis; 2002. p. 143–59.

    Google Scholar 

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Acknowledgments

We thank officials of Spices Board and Agricultural Department, Kodagu District, Karnataka and Idukki District, Kerala for their assistance during survey. We also thank The Director, Indian Institute of Spices Research, Calicut and The Head, IISR, Cardamom Research Centre, Appangala, Madikeri for providing the facilities.

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Affiliations

  1. Indian Institute of Spices Research, Cardamom Research Centre, Appangala, Heravanadu Post, Madikeri, 571 201, Karnataka, India

    C. N. Biju

  2. Division of Crop Protection, Indian Institute of Spices Research, Marikunnu, Calicut, 673 012, Kerala, India

    A. Siljo & A. I. Bhat

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  1. C. N. Biju
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  2. A. Siljo
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  3. A. I. Bhat
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Correspondence to A. I. Bhat.

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Biju, C.N., Siljo, A. & Bhat, A.I. Survey and RT-PCR Based Detection of Cardamom mosaic virus Affecting Small Cardamom in India. Indian J. Virol. 21, 148–150 (2010). https://doi.org/10.1007/s13337-011-0026-3

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  • DOI: https://doi.org/10.1007/s13337-011-0026-3

Keywords

  • Small cardamom
  • Katte
  • Mosaic
  • Incidence
  • Distribution
  • Cardamom mosaic virus
  • RT-PCR
  • Detection