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Biodistribution and Pharmacokinetic Study of 3,3′ Diseleno Dipropionic Acid (DSePA), A Synthetic Radioprotector, in Mice

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Abstract

Background and Objectives

3,3′ Diseleno dipropionic acid (DSePA), a synthetic compound has been shown to have radioprotective activity, especially as a lung radioprotector. In this study, the pharmacokinetics and biodistribution of DSePA in MX-1 tumour bearing SCID mice were evaluated.

Methods

Twenty SCID mice were administered DSePA (50 mg/kg bodyweight) by oral gavage following which four animals each were sacrificed at 15, 30 min, 1, 2 and 4 h. Blood and tissue samples were collected for determination of DSePA concentration by graphite furnace atomic absorption spectrometry (GFAAS) method. The control group (n = 4) was administered sterile water and sacrificed at 4 h.

Results

Peak plasma concentration (C max) of 2.7 µg/ml was observed at 15 min which returned to near baseline (baseline = 0.6 µg/ml) at 1 h following drug administration. Biphasic pharmacokinetics characterized by rapid distribution phase and a slower elimination phase were observed. Highest maximal concentration (C max) of the drug was observed in lung (19.2 µg/g at 30 min) followed by intestine (14.64 µg/g at 15 min) and kidney (12.96 µg/g at 15 min). There was negligible uptake in tumor tissue and no uptake in brain.

Conclusions

DSePA has a favorable pharmacokinetic profile which makes it a potentially good candidate for further development as a radioprotective agent.

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Authors and Affiliations

  1. Department of Clinical Pharmacology, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, 410210, India

    Vikram Gota, Kartik Doshi & Anand Patil

  2. Department of Radiation Oncology, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, India

    Jayant S. Goda

  3. Analytical Chemistry Division, Bhabha Atomic Research Centre, Mumbai, India

    Suvarna Sunderajan & Kiran Kumar

  4. Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, India

    Mahendra Varne, Amit Kunwar & Indira Priyadarshini

  5. Chemistry Division, Bhabha Atomic Research Centre, Mumbai, India

    Vimal Kumar Jain

Authors
  1. Vikram Gota
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  2. Jayant S. Goda
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  3. Kartik Doshi
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  4. Anand Patil
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  5. Suvarna Sunderajan
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  6. Kiran Kumar
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  7. Mahendra Varne
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  8. Amit Kunwar
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  9. Vimal Kumar Jain
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  10. Indira Priyadarshini
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Corresponding author

Correspondence to Vikram Gota.

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Funding

No sources of funding were used to conduct this study.

Conflict of interest

All authors have no conflicts of interest to declare.

Ethical approval

The study was approved by the Institutional Animal Ethics Committee of ACTREC.

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Gota, V., Goda, J.S., Doshi, K. et al. Biodistribution and Pharmacokinetic Study of 3,3′ Diseleno Dipropionic Acid (DSePA), A Synthetic Radioprotector, in Mice. Eur J Drug Metab Pharmacokinet 41, 839–844 (2016). https://doi.org/10.1007/s13318-015-0301-6

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  • DOI: https://doi.org/10.1007/s13318-015-0301-6

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