Abstract
Astragalus strictus (Fabaceae) is one of the major locoweeds commonly found in Tibet, China. In 2021 and 2022, the occurrence of powdery mildew on A. strictus leaves was investigated in Tibet with approximately 50‒70% disease incidence and 26.9‒37.5% disease severity. The typical symptoms included white powdery mildew colonies and dark chasmothecia on the host leaves. To identify the causal agent, fresh leaves covered with powdery mildew were collected and identified by morphology and molecular analyses. Phylogenetic analyses of the internal transcribed spacer (ITS) region and the 28 S (large subunit) rDNA showed that our collection was clustered with Erysiphe astragali. The morphology of our collection was appendages equatorially and flexuous, asci 4–8 per chasmothecium, ovoid to subglobose and ascospores 4 per ascus, ovoid or ellipsoid. A pathogenicity test on plants by pressing naturally infected leaves onto healthy leaves confirmed that the tested fungus was pathogenic. Therefore, E. astragali was identified as the causative agent of this disease. To our knowledge, this is the first report of E. astragali associated with powdery mildew on A. strictus in China.
Similar content being viewed by others
References
Beenken L (2017) First records of the powdery mildews Erysiphe platani and E. alphitoides on Ailanthus altissima reveal host jumps independent of host phylogeny Mycol. Prog. 16:135–143. https://doi.org/10.1007/s11557-016-1260-2
Bhatia A, Chug A, Singh AP (2021) Statistical analysis of machine learning techniques for predicting powdery mildew disease in tomato plants. Int J Intelligent Engineering Informatics 9(1):24–58. https://doi.org/10.1504/ijiei.2021.116087
Bradshaw M, Braun U, Götz M, Jurick ΙΙW (2022) Phylogeny and taxonomy of powdery mildew caused by Erysiphe species on Lupinus hosts. Mycologia 114(1):76–88. https://doi.org/10.1080/00275514.2021.1973287
Bradshaw M, Braun U, Meeboon J, Tobin P (2021) Phylogeny and taxonomy of powdery mildew caused by Erysiphe species on Corylus hosts. Mycologia 113(2):459–475. https://doi.org/10.1080/00275514.2020.1837568
Bradshaw M, Braun U, Wang S, Liu S, Feng J, Shin HD, Choi YJ, Takamatsu S, Bulgakov TS, Tobin PC (2020) Phylogeny and taxonomy of powdery mildew on Viburnum species. Mycologia 112(3):616–632. https://doi.org/10.1080/00275514.2020.1739508
Braun U, Cook RTA (2012) Taxonomic manual of the Erysiphales (powdery mildews). Utrecht: CBS-KNAW Fungal Biodiversity Centre, No. 11. CBS, Utrecht, the Netherlands, pp 1–707
Braun U (1987) A monograph of the Erysiphales (powdery mildews). Beih Nova Hedwigia 89:1–700
Cortinovis C, Caloni F (2017) Plants toxic to farm and companion animals. In: Carlini C, Ligabue-Braun R (eds) Plant toxins. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6464-4_23
Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9(8):772. https://doi.org/10.1038/nmeth.2109
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Dunn MW, Gaynor LG (2020) Impact and control of powdery mildew on irrigated soybean varieties grown in Southeast Australia. Agronomy 10:514. https://doi.org/10.3390/agronomy10040514
Ellingham O, David J, Culham A (2019) Enhancing identification accuracy for powdery mildews using previously underexploited DNA loci. Mycologia 111(5):798–812. https://doi.org/10.1080/00275514.2019.1643644
Enright SM, Cipollini D (2007) Infection by powdery mildew Erysiphe cruciferarum (Erysiphaceae) strongly affects growth and fitness of Alliaria petiolata (Brassicaceae). Mycol Plant Patholo 94(11):1813–1820. https://doi.org/10.3732/ajb.94.11.1813
Farr DF, Rossman AY (2022) Fungal Databases, U.S. National Fungus Collections. Available online at: https://nt.ars-grin.gov/fungaldatabases/. Accessed 7 December 2022
Jayawardena RS, Hyde KD, Chen YJ, Papp V, Palla B, Papp D, Bhunjun CS, Hurdeal VG, Senwanna C, Manawasinghe IS et al (2020) One stop shop IV: taxonomic update with molecular phylogeny for important phytopathogenic genera: 76–100 (2020). Fungal Divers 103:87–218. https://doi.org/10.1007/s13225-020-00460-8
Johnston PR, Quijada L, Smith CA, Baral HO, Hosoya T, Baschien C, Pärtel K, Zhuang WY, Haelewaters D, Park D et al (2019) A multigene phylogeny toward a new phylogenetic classification of Leotiomycetes. IMA Fungus 10:1–22. https://doi.org/10.1186/s43008-019-0002-x
Kirschner R (2010) First record of Erysiphe magnifica on lotus, a host outside the Magnoliales. Mycol Prog 9:417–424. https://doi.org/10.1007/s11557-009-0651-z
Liu L, Bradshaw M, Braun U, Götz M, Khodaparast SA, Liu TZ, Bulgakov TS, Darsaraei H, Hofbauer WK, Li Y, Liu SY (2022) Phylogeny and taxonomy of Erysiphe berberidis (s. lat.) Revisited. Mycoscience 63(5):222–234. https://doi.org/10.47371/mycosci.2022.07.005
Miller MA, Pfeiffer W, Schwartz T (2017) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 gateway computing environments workshop (GCE), pp 1–8
Nasir M, Mughal SM, Mukhtar T, Awan MZ (2014) Powdery mildew of mango: a review of ecology, biology, epidemiology and management. Crop Prot 64:19–26. https://doi.org/10.1016/j.cropro.2014.06.003
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:312–1313. https://doi.org/10.1093/bioinformatics/btu033
Takamatsu S, Matsuda S, Grigaliunaite B (2013) Comprehensive phylogenetic analysis of the genus Golovinomyces (Ascomycota: Erysiphales) reveals close evolutionary relationships with its host plants. Mycologia 105(5):1135–1152. https://doi.org/10.3852/13-046
Takamatsu S, Ito H, Shiroya Y, Kiss L, Heluta V (2015) First comprehensive phylogenetic analysis of the genus Erysiphe (Erysiphales, Erysiphaceae) I. The Microsphaera lineage. Mycologia 107:475–489. https://doi.org/10.3852/15-007
Takamatsu S (2018) Studies on the evolution and systematics of powdery mildew fungi. J Gen Plant Pathol 84:422–426. https://doi.org/10.1007/s10327-018-0805-4
Tymon LS, Bradshaw M, Götz M, Braun U, Peever TL, Edmonds RL (2022) Phylogeny and taxonomy of Erysiphe spp. on Rhododendron, with a special emphasis on north american species. Mycologia 114(5):887–899. https://doi.org/10.1080/00275514.2022.2090219
Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified DNA from several Cryptococcus species. J Bacteriol 172:4238–4246. https://doi.org/10.1128/JB.172.8.4238-4246.1990
Wang Z, Binder M, Schoch CL, Johnston PR, Spatafora JW, Hibbett DS (2006) Evolution of helotialean fungi (Leotiomycetes, Pezizomycotina): a nuclear rDNA phylogeny. Mol Phylogenet Evol 41:295–312. https://doi.org/10.1016/j.ympev.2006.05.031
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 18(1):315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Yang C, Sha RK, Surilage, Wang S, Mo CH, Wu CC, Lu H, Zhao BY (2022) Research progress on distribution, toxicity, control and utilization of poisonous grass species in natural grassland of China. J Domest Anim Ecol 11:88–96 Chinese
Zhao BY, Fan YY, Fan ZF, Dong Q, Cao HM, Li GZ, Da NT (2006) Hazards of locoweed in the west grassland of China and the control of animals loco-toxicosis. Grass-Feeding Livest 130:12–15 Chinese
Zhao BY, Liu XY, Wang JJ, Li S (2009) Isolation and NMR study on Swainsonine from locoweed, Astragalus strictus. Agricultural Sci China 8:115–120
Zhao HH, Xing HH, Liang C, Yang XY, Cho SE, Shin HD (2014) First report of powdery mildew caused by Erysiphe cruciferarum on Chinese cabbage in China. Plant Dis 98(3):421–421. https://doi.org/10.1094/pdis-06-13-0648-pdn
Acknowledgements
This research was funded by the National Microbial Resource Centre of the Ministry of Science and Technology of the People’s Republic of China (NMRC-2022-7). We thank Min Liu, Yuanyuan Liu, Peng Jin and Shoucheng Wan for the help in the disease investigation.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Wang, CB., Jiang, N. First report of Erysiphe astragali causing powdery mildew on Astragalus strictus in Tibet, China. Australasian Plant Pathol. 52, 207–214 (2023). https://doi.org/10.1007/s13313-023-00911-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13313-023-00911-1