Abstract
Cercosporin, a nonspecific toxin produced by Cercospora spp., Pseudocercosporella spp. and Colletotrichum spp., is a photosensitizing compound that rapidly kills plant cells in the presence of light. Although this toxin is a virulence factor, its quantification in coffee leaves has not previously been reported. This study aimed to standardize and quantify cercosporin in the leaves of Coffea arabica cultivars (Mundo Novo 376/4, Catuaí Vermelho IAC 99, Topázio MG 1190, and IPR 102) and Coffea canephora (Conilon- clone 213) with brown eye spot symptoms using high-performance liquid chromatography (HPLC). Cercosporin was detected in the lesions of all examined cultivars. Thus, HPLC is a valid method for quantifying cercosporin in coffee leaves.
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Acknowledgements
To National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), the Research Support Foundation of the State of Minas Gerais (FAPEMIG), and the National Institute for Coffee Science and Technology (INCT-Café) for supporting this research.
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Ramos, J.B., de Resende, M.L.V., Andrade, M.E.R. et al. Quantification of cercosporin from coffee leaves infected by Cercospora coffeicola. Australasian Plant Pathol. 51, 429–432 (2022). https://doi.org/10.1007/s13313-022-00868-7
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DOI: https://doi.org/10.1007/s13313-022-00868-7