Abstract
Chrysoporthe puriensis, a sibling species of the well-known Eucalyptus canker pathogen Chr. cubensis, has recently been described from Brazil. Both species are thought to be native to South America, but previous population genetic analyses were conducted prior to the ready availability of robust markers such as microsatellites to test this hypothesis. The objective of this investigation was to analyse the structure and genetic variability of Chr. cubensis and Chr. puriensis populations from non-native Myrtaceae and native Melastomataceae hosts in Brazil, using microsatellite markers developed for this purpose. The fungal isolates were obtained from Eucalyptus species, Corymbia citriodora and Tibouchina species in different regions of the country. Isolates were separated into sub-populations based on host families (Melastomataceae and Myrtaceae) and on region of origin. There was high genetic variability in all sub-populations with the highest levels detected within, rather than among sub-populations. Gene and genotypic diversities were higher for the isolates from the Melastomataceae than the Myrtaceae isolates. High levels of gene flow were found between sub-populations based on host and geographic distribution of the sub-populations. The presence of genetically diverse Chr. cubensis and Chr. puriensis populations on native hosts in Brazil supports a Latin American centre of origin for the two pathogens. Both undergo sexual and asexual reproduction and have a high potential for gene flow.
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Data openly available in a public repository that does not issue DOIs.
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Acknowledgements
We are grateful to members of the Tree Protection Co-operative Programme (TPCP) and the DST/NRF Centre of Excellence in Tree Health Biotechnology (South Africa) for financial support. We also thank the FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for providing scholarships to Mara Elisa Soares de Oliveira and Gabrielle Avelar Silva. We thank to ICMBio for authorizing the collection of samples and the Parks: Serra da Canastra National, Itatiaia National, Chapada Diamantina National and Quedas do Rio Bonito, for receiving us for field collections.
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The data that support the findings will be available in NCBI Nucleotide at https://www.re3data.org/search?query=ncbi. The Genbank numbers are given in the text of the manuscript. The data is under embargo until a manuscript is accepted for publication. It will then be openly available. Should reviewers wish to see the data they will be made available.
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Oliveira, M.E.S., Kanzi, A.M., van der Merwe, N.A. et al. Genetic variability in populations of Chrysoporthe cubensis and Chr. puriensis in Brazil. Australasian Plant Pathol. 51, 175–191 (2022). https://doi.org/10.1007/s13313-021-00847-4
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DOI: https://doi.org/10.1007/s13313-021-00847-4