Abstract
Solidago microglossa (goldenrod) is a species with medicinal properties, generating products of estimable economic value, which are commercialized as medicines and cosmetics. Plants of S. microglossa exhibiting intense proliferation of small leaves, producing a compact canopy, were found sparsely in a field. Since these symptoms are indicative of infection by phytoplasmas, a survey was performed in order to understand the etiological cause of observed symptoms. The total DNA extracted from the tested plants was used in nested PCR primed by P1/16S-SR followed by R16F2n/R16R2. Leaf veins were appropriately prepared for visualization in transmission electron microscopy. The presence of phytoplasma was revealed in symptomatic samples by amplification of genomic fragments of 1.25 Kb. In agreement, phytoplasma cells were observed in the phloem vessels of these plants. Nucleotide sequences from the phytoplasma were indistinguishable among themselves and shared 99.7% identity with sequences of phytoplasmas belonging to the 16SrIII group. Computer-simulated RFLP and phylogenetic analysis classified the S. microglossa phytoplasma as member of the 16SrIII-X subgroup. In addition, the present study revealed S. microglossa as an additional host species for this phytoplasma.
References
Bertaccini A, Duduk B, Paltrinieri S, Contaldo N (2014) Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. Am J Plant Sci 5:1763–1788
Deng S, Hiruki C (1991) Amplification of 16S rRNA genes from culturable and non-culturable Mollicutes. J Microbiol Methods 14:53–61
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Fernández FD, Meneguzzi NG, Conci LR (2017) Identification of three novel subgroups within the X-disease group phytoplasma associated with strawberry redness disease. Int J Syst Evol Microbiol 67:753–758
Flôres D, Bedendo IP, Kitajima EW (2011) Molecular identification of a phytoplasma belonging to the 16SrIII group associated with Solidago microglossa. Summa Phytopathol 37:148 (Supplement)
Gadelha CS, Pinto Junior VM, Bezerra KKS, Maracajá PB, Martins DSS (2015) Use of herbal medicines and medicinal plants in different segments of society. Green J Agroecol Sustain Dev 10:01–15
Galdeano E, Guzmán FA, Fernández F, Conci LR (2013) Genetic diversity of 16SrIII group phytoplasmas in Argentina. Predominance of subgroups 16SrIII-J and B and two new subgroups 16SrIII-W and X. Eur J Plant Pathol 137:753–764
Gundersen DE, Lee I-M (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr 35:144–151
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Lee IM, Martini M, Marcone C, Zhu SF (2004) Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of ‘Candidatus phytoplasma ulmi’ for the phytoplasma associated with elm yellows. Int J Syst Evol Microbiol 54:337–347
Lee IM, Zhao Y, Davis RE (2010) Prospects of multiple gene-based systems for differentiation and classification of phytoplasmas. In: Weintraub PG, Jones P (eds) Genomes Plant Hosts and Vectors. CAB International, Wallingford Oxfordshire, pp 51–63
Lorenzi H, Matos FJ (2002) Plantas medicinais no Brasil: Nativas e Exóticas. Instituto Plantarum, Nova Odessa, Brazil
Maunsbach AB, Afzelius BA (1998) Biomedical electron microscopy: Illustrated Methods and Interpretations. Elsevier, San Diego
Oliveira F, Akisue G, Akisue MK (1991) Pharmacognosy. Atheneu, São Paulo
Oliveira FF, Ferreira J, Galvão SR, Kitajima EW, Bedendo IP (2020) Chrysanthemum is a new host of a group 16SrIII phytoplasma (16SrIII-X) that induces colour-breaking in affected plants. Eur J Plant Pathol 157:699–705
Pérez-López E, Luna-Rodríguez M, Olivier CY, Dumonceaux TJ (2016) The underestimated diversity of phytoplasmas in Latin America. Int J Syst Evol Microbiol 66:492–513
Semple JC (2015) Solidago L.: Classification and llustrations of Goldenrods. University of Waterloo, Waterloo, Ontario. https://uwaterloo.ca/astereae-lab/research/goldenrods/classification-and-illustrations. Accessed 17 Apr 2020
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2727
Wei W, Davis RE, Lee IM, Zhao Y (2007) Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. Int J Syst Evol Microbiol 57:1855–1867
Wei W, Lee IM, Davis RE, Suo X, Zhao Y (2008) Automated RFLP pattern comparison and similarity coefficient calculation for rapid delineation of new and distinct phytoplasma subgroup lineages. Int J Syst Evol Microbiol 58:2368–2377
Zhao Y, Wei W, Lee IM, Shao J, Suo X, Davis RE (2009) Construction of an interactive on line phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X- disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 59:2582–2593
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The present research was supported by The Brazilian National Council for Scientific and Technological Development (CNPq), Project 304268/2018-6.
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All authors contributed to the study conception, design, material preparation, data collection and analysis. The manuscript was written by J. Ferreira and I.P. Bedendo and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ferreira, J., de Almeida, C.A., Kitajima, E.W. et al. A subgroup 16SrIII-X phytoplasma is associated with small leaf proliferation in goldenrod plants. Australasian Plant Pathol. 51, 295–299 (2022). https://doi.org/10.1007/s13313-021-00842-9
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DOI: https://doi.org/10.1007/s13313-021-00842-9