Abstract
Citrus tristeza virus was detected for the first time in the Lao People’s Democratic Republic. Samples were collected from citrus trees across the southern provinces for testing in Australia. RNA was extracted and tested using conventional and real-time reverse transcription polymerase chain reactions with the virus detected in 12 of 59 samples tested. Viral identities were confirmed by sequencing. Additional confirmation was obtained by an enzyme-linked immunosorbent assay. The implications of the presence of this virus for citrus production in Lao are discussed briefly.
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Acknowledgements
The authors gratefully acknowledge support from the Champasak Provincial Agriculture and Forestry Office, the Lao PDR Department of Agriculture, the Crawford Fund of Australia, the Australian Centre for International Agricultural Research (ACIAR), the NSW Department of Primary Industries, Western Sydney University and Hort Innovation (CT17007 Improving diagnostics and biosecurity for graft-transmissible diseases in citrus), using the citrus research and development levy and contributions from the Australian Government. Hort Innovation is the grower owned, not-for-profit research and development corporation for Australian horticulture. The fifth author was a short-term Volunteer Plant Pathologist in Champasak Province (PAFO) through the Australian Government Volunteer Program. The authors gratefully acknowledge John Thomas, Andrew Geering (The University of Queensland) and Bernie Dominiak (NSW Department of Primary Industries) for critical review of the manuscript.
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Donovan, N.J., Englezou, A., Chambers, G.A. et al. First report of citrus tristeza virus in Lao PDR. Australasian Plant Pathol. 50, 683–685 (2021). https://doi.org/10.1007/s13313-021-00818-9
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DOI: https://doi.org/10.1007/s13313-021-00818-9